Reviewed, UniProtKB/Swiss-Prot Q29490 (ALDH1_MACPR)

Names and origin

Protein names

Recommended name:
    Aldehyde dehydrogenase, cytosolic 1
    EC=1.2.1.3
Alternative name(s):
    ALDH class 1
    ETA-crystallin

Gene names

Name:

ALDH1

Organism

Macroscelides proboscideus (Short-eared elephant shrew)

Taxonomic identifier

29082 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Afrotheria › Macroscelidea › Macroscelididae › Macroscelides

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Major component of the eye of elephant shrews, which in contrast to other mammals, possesses both a lens- and a non-lens class-1 aldehyde dehydrogenase 1. This eye-specific form is a structural protein of the lens and, in other part of the eye, serves as the major form of ALDH1. Can convert/oxidize retinaldehyde to retinoic acid.
Catalytic activity	An aldehyde + NAD+ + H2O = an acid + NADH.
Pathway	Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Tissue specificity	Eye specific, with very high expression in the lens.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aldehyde dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Aldehyde dehydrogenase, cytosolic 1		PRO_0000056428
Regions
	Nucleotide binding	246 – 251	6	NAD By similarity
Sites
	Active site	269	1	Proton acceptor By similarity
	Active site	303	1	Nucleophile By similarity
	Site	170	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q29490-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 48C15C682665D0C0	FASTA	501	54,625
	10 20 30 40 50 60 MSSSGMPDLP APLTNIKIQH TKLFINNEWH DSVSGKTFPV FNPATEEKIC EVEEADKEDV 70 80 90 100 110 120 DKAVKAAREA FQMGSPWRTM DASERGQLIY KLADLIERDR LLLATLESIN AGKIFASAYL 130 140 150 160 170 180 MDLDYCIKVL RYCAGWADKI QGRTIPVDGE FFSYTRHEPI GVCGQIFPWN APMILLACKI 190 200 210 220 230 240 GPALCCGNTV IVKPAEQTPL TALHVASLIK EAGFPPGVVN IVPGYGPTAG AAISSHMDVD 250 260 270 280 290 300 KVAFTGSTEV GKMIQEAAAK SNLKRVTLEL GAKNPCIVFA DADLDSAVEF AHQGVFTNQG 310 320 330 340 350 360 QSCIAASKLF VEETIYDEFV QRSVERAKKY VFGNPLTPGV NHGPQINKAQ HNKIMELIES 370 380 390 400 410 420 GKKEGAKLEC GGGPWGNKGY FIQPTIFSNV TDDMRIAKEE IFGPVQQIMK FKSLDEVIKR 430 440 450 460 470 480 ANNTYYGLVA GVFTKDLDKA VTVSSALQAG TVWVNCYLAA SAQSPAGGFK MSGHGREMGE 490 500 YGIHEYTEVK TVTMKISEKN S

References

[1]	"A retinaldehyde dehydrogenase as a structural protein in a mammalian eye lens. Gene recruitment of eta-crystallin." Graham C., Hodin J., Wistow G. J. Biol. Chem. 271:15623-15628(1996) [PubMed: 8663049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Eye.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U03906 mRNA. Translation: AAC48588.1.
3D structure databases
HSSP	HSSP built from PDB template 1O9J based on UniProtKB Q28399.
ModBase	Search...
Phylogenomic databases
HOVERGEN	Q29490.
Enzyme and pathway databases
BRENDA	1.2.1.3. 317919.
Family and domain databases
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALDH1_MACPR

Accession

Primary (citable) accession number: Q29490

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)