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Q29486

- PPBT_FELCA

UniProt

Q29486 - PPBT_FELCA

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Protein

Alkaline phosphatase, tissue-nonspecific isozyme

Gene

ALPL

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Binds 1 magnesium ion.By similarity
Binds 2 zinc ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi60 – 601MagnesiumBy similarity
Metal bindingi60 – 601Zinc 1By similarity
Active sitei110 – 1101Phosphoserine intermediatePROSITE-ProRule annotation
Metal bindingi110 – 1101Zinc 1By similarity
Metal bindingi173 – 1731MagnesiumBy similarity
Metal bindingi332 – 3321MagnesiumBy similarity
Metal bindingi337 – 3371Zinc 2By similarity
Metal bindingi341 – 3411Zinc 2; via tele nitrogenBy similarity
Metal bindingi378 – 3781Zinc 1By similarity
Metal bindingi379 – 3791Zinc 1; via tele nitrogenBy similarity
Metal bindingi454 – 4541Zinc 2; via tele nitrogenBy similarity

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase, tissue-nonspecific isozyme (EC:3.1.3.1)
Short name:
AP-TNAP
Short name:
TNSALP
Alternative name(s):
Alkaline phosphatase liver/bone/kidney isozyme
Gene namesi
Name:ALPL
OrganismiFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifieri9685 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
ProteomesiUP000011712: Unplaced

Subcellular locationi

Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Add
BLAST
Chaini18 – 502485Alkaline phosphatase, tissue-nonspecific isozymePRO_0000024021Add
BLAST
Propeptidei503 – 52422Removed in mature formSequence AnalysisPRO_0000024022Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi139 ↔ 201By similarity
Glycosylationi140 – 1401N-linked (GlcNAc...)Sequence Analysis
Glycosylationi230 – 2301N-linked (GlcNAc...)Sequence Analysis
Glycosylationi271 – 2711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi302 – 3021N-linked (GlcNAc...)Sequence Analysis
Glycosylationi430 – 4301N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi489 ↔ 497By similarity
Lipidationi502 – 5021GPI-anchor amidated serineSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9685.ENSFCAP00000002729.

Structurei

3D structure databases

ProteinModelPortaliQ29486.
SMRiQ29486. Positions 18-498.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG1785.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiQ29486.
KOiK01077.
OrthoDBiEOG7SN8CH.
TreeFamiTF323513.

Family and domain databases

Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q29486-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MISPFLVLAI GTCLTNSLVP EKEKDPKYWR DQAQQTLKNA LRLQKLNTNV
60 70 80 90 100
VKNVIMFLGD GMGVSTVTAA RILKGQLHHN PGEETRLEMD KFPYVALSKT
110 120 130 140 150
YNTNAQVPDS AGTATAYLCG VKANEGTVGV SAATQRTQCN TTQGNEVTSI
160 170 180 190 200
LRWAKDSGKS VGIVTTTRVN HATPSAAYAH SADRDWYSDN EMPPEALSQG
210 220 230 240 250
CKDIAYQLMH NVRDIEVIMG GGRKYMFPKN RTDVEYEMDE KARGTRLDGL
260 270 280 290 300
NLVDIWKSFK PRHKHSHYVW NRTELLTLDP YGVDYLLGLF EPGDMQYELN
310 320 330 340 350
RNSTTDPSLS EMVEIAIKIL SKNPKGFFLL VEGGRIDHGH HEGKAKQALH
360 370 380 390 400
EAVEMDQAIG RAGAMTSVED TLTIVTADHS HVFTFGGYTP RGNSIFGLAP
410 420 430 440 450
MVSDTDKKPF TSILYGNGPG YKVVGGEREN VSMVDYAHNN YQAQSAVPLR
460 470 480 490 500
HETHGGEDVA VFAKGPMAHL LHGVHEQNYI PHVMAYAACI GANLDHCASA
510 520
SSAGGPSPGP LFLLLALPSL GILF
Length:524
Mass (Da):57,345
Last modified:November 1, 1997 - v1
Checksum:iF4863A03C7882A72
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U31569 mRNA. Translation: AAA82993.1.
PIRiS66467.
RefSeqiNP_001036028.1. NM_001042563.1.

Genome annotation databases

GeneIDi727689.
KEGGifca:727689.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U31569 mRNA. Translation: AAA82993.1 .
PIRi S66467.
RefSeqi NP_001036028.1. NM_001042563.1.

3D structure databases

ProteinModelPortali Q29486.
SMRi Q29486. Positions 18-498.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9685.ENSFCAP00000002729.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 727689.
KEGGi fca:727689.

Organism-specific databases

CTDi 249.

Phylogenomic databases

eggNOGi COG1785.
HOGENOMi HOG000099118.
HOVERGENi HBG007345.
InParanoidi Q29486.
KOi K01077.
OrthoDBi EOG7SN8CH.
TreeFami TF323513.

Family and domain databases

Gene3Di 3.40.720.10. 1 hit.
InterProi IPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view ]
Pfami PF00245. Alk_phosphatase. 1 hit.
[Graphical view ]
PRINTSi PR00113. ALKPHPHTASE.
SMARTi SM00098. alkPPc. 1 hit.
[Graphical view ]
SUPFAMi SSF53649. SSF53649. 1 hit.
PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "cDNA encoding a functional feline liver/bone/kidney-type alkaline phosphatase."
    Ghosh A.K., Mullins J.I.
    Arch. Biochem. Biophys. 322:240-249(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.

Entry informationi

Entry nameiPPBT_FELCA
AccessioniPrimary (citable) accession number: Q29486
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3