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Q29486

- PPBT_FELCA

UniProt

Q29486 - PPBT_FELCA

Protein

Alkaline phosphatase, tissue-nonspecific isozyme

Gene

ALPL

Organism
Felis catus (Cat) (Felis silvestris catus)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 1 magnesium ion.By similarity
    Binds 2 zinc ions.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi60 – 601MagnesiumBy similarity
    Metal bindingi60 – 601Zinc 1By similarity
    Active sitei110 – 1101Phosphoserine intermediatePROSITE-ProRule annotation
    Metal bindingi110 – 1101Zinc 1By similarity
    Metal bindingi173 – 1731MagnesiumBy similarity
    Metal bindingi332 – 3321MagnesiumBy similarity
    Metal bindingi337 – 3371Zinc 2By similarity
    Metal bindingi341 – 3411Zinc 2; via tele nitrogenBy similarity
    Metal bindingi378 – 3781Zinc 1By similarity
    Metal bindingi379 – 3791Zinc 1; via tele nitrogenBy similarity
    Metal bindingi454 – 4541Zinc 2; via tele nitrogenBy similarity

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline phosphatase, tissue-nonspecific isozyme (EC:3.1.3.1)
    Short name:
    AP-TNAP
    Short name:
    TNSALP
    Alternative name(s):
    Alkaline phosphatase liver/bone/kidney isozyme
    Gene namesi
    Name:ALPL
    OrganismiFelis catus (Cat) (Felis silvestris catus)
    Taxonomic identifieri9685 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraFeliformiaFelidaeFelinaeFelis
    ProteomesiUP000011712: Unplaced

    Subcellular locationi

    Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Add
    BLAST
    Chaini18 – 502485Alkaline phosphatase, tissue-nonspecific isozymePRO_0000024021Add
    BLAST
    Propeptidei503 – 52422Removed in mature formSequence AnalysisPRO_0000024022Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi139 ↔ 201By similarity
    Glycosylationi140 – 1401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi230 – 2301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi271 – 2711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi302 – 3021N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi430 – 4301N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi489 ↔ 497By similarity
    Lipidationi502 – 5021GPI-anchor amidated serineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi9685.ENSFCAP00000002729.

    Structurei

    3D structure databases

    ProteinModelPortaliQ29486.
    SMRiQ29486. Positions 18-498.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alkaline phosphatase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1785.
    HOGENOMiHOG000099118.
    HOVERGENiHBG007345.
    KOiK01077.
    OrthoDBiEOG7SN8CH.
    TreeFamiTF323513.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view]
    PfamiPF00245. Alk_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00113. ALKPHPHTASE.
    SMARTiSM00098. alkPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q29486-1 [UniParc]FASTAAdd to Basket

    « Hide

    MISPFLVLAI GTCLTNSLVP EKEKDPKYWR DQAQQTLKNA LRLQKLNTNV    50
    VKNVIMFLGD GMGVSTVTAA RILKGQLHHN PGEETRLEMD KFPYVALSKT 100
    YNTNAQVPDS AGTATAYLCG VKANEGTVGV SAATQRTQCN TTQGNEVTSI 150
    LRWAKDSGKS VGIVTTTRVN HATPSAAYAH SADRDWYSDN EMPPEALSQG 200
    CKDIAYQLMH NVRDIEVIMG GGRKYMFPKN RTDVEYEMDE KARGTRLDGL 250
    NLVDIWKSFK PRHKHSHYVW NRTELLTLDP YGVDYLLGLF EPGDMQYELN 300
    RNSTTDPSLS EMVEIAIKIL SKNPKGFFLL VEGGRIDHGH HEGKAKQALH 350
    EAVEMDQAIG RAGAMTSVED TLTIVTADHS HVFTFGGYTP RGNSIFGLAP 400
    MVSDTDKKPF TSILYGNGPG YKVVGGEREN VSMVDYAHNN YQAQSAVPLR 450
    HETHGGEDVA VFAKGPMAHL LHGVHEQNYI PHVMAYAACI GANLDHCASA 500
    SSAGGPSPGP LFLLLALPSL GILF 524
    Length:524
    Mass (Da):57,345
    Last modified:November 1, 1997 - v1
    Checksum:iF4863A03C7882A72
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U31569 mRNA. Translation: AAA82993.1.
    PIRiS66467.
    RefSeqiNP_001036028.1. NM_001042563.1.

    Genome annotation databases

    GeneIDi727689.
    KEGGifca:727689.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U31569 mRNA. Translation: AAA82993.1 .
    PIRi S66467.
    RefSeqi NP_001036028.1. NM_001042563.1.

    3D structure databases

    ProteinModelPortali Q29486.
    SMRi Q29486. Positions 18-498.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9685.ENSFCAP00000002729.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 727689.
    KEGGi fca:727689.

    Organism-specific databases

    CTDi 249.

    Phylogenomic databases

    eggNOGi COG1785.
    HOGENOMi HOG000099118.
    HOVERGENi HBG007345.
    KOi K01077.
    OrthoDBi EOG7SN8CH.
    TreeFami TF323513.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view ]
    Pfami PF00245. Alk_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00113. ALKPHPHTASE.
    SMARTi SM00098. alkPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA encoding a functional feline liver/bone/kidney-type alkaline phosphatase."
      Ghosh A.K., Mullins J.I.
      Arch. Biochem. Biophys. 322:240-249(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.

    Entry informationi

    Entry nameiPPBT_FELCA
    AccessioniPrimary (citable) accession number: Q29486
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 93 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3