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Reviewed, UniProtKB/Swiss-Prot Q29486 (PPBT_FELCA)

Last modified February 9, 2010. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alkaline phosphatase, tissue-nonspecific isozyme
      Short name=AP-TNAP
      Short name=TNSALP
    EC=3.1.3.1
Alternative name(s):
    Alkaline phosphatase liver/bone/kidney isozyme
Gene names
Name: ALPL
OrganismFelis catus (Cat) (Felis silvestris catus)
Taxonomic identifier9685 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis

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Protein attributes

Sequence length524 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion By similarity.

Binds 2 zinc ions By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Cell membrane; Lipid-anchor › GPI-anchor By similarity.

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Sequence similarities

Belongs to the alkaline phosphatase family.

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Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainSignal
   LigandMagnesium
Metal-binding
Zinc
   Molecular functionHydrolase
   PTMGPI-anchor
Glycoprotein
Lipoprotein
Phosphoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processmetabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentanchored to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionalkaline phosphatase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717
Chain18 – 502485Alkaline phosphatase, tissue-nonspecific isozyme
PRO_0000024021
Propeptide503 – 52422Removed in mature form Potential
PRO_0000024022

Sites

Active site1101Phosphoserine intermediate By similarity
Metal binding601Magnesium Potential
Metal binding601Zinc 2 Potential
Metal binding1731Magnesium Potential
Metal binding3321Magnesium Potential
Metal binding3371Zinc 1 Potential
Metal binding3411Zinc 1 Potential
Metal binding3781Zinc 2 Potential
Metal binding3791Zinc 2 Potential
Metal binding4541Zinc 1 Potential

Amino acid modifications

Lipidation5021GPI-anchor amidated serine Potential
Glycosylation1401N-linked (GlcNAc...) Potential
Glycosylation2301N-linked (GlcNAc...) Potential
Glycosylation2711N-linked (GlcNAc...) Potential
Glycosylation3021N-linked (GlcNAc...) Potential
Glycosylation4301N-linked (GlcNAc...) Potential

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Sequences

Sequence LengthMass (Da)Tools
Q29486-1 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: F4863A03C7882A72

FASTA52457,345
        10         20         30         40         50         60 
MISPFLVLAI GTCLTNSLVP EKEKDPKYWR DQAQQTLKNA LRLQKLNTNV VKNVIMFLGD 

        70         80         90        100        110        120 
GMGVSTVTAA RILKGQLHHN PGEETRLEMD KFPYVALSKT YNTNAQVPDS AGTATAYLCG 

       130        140        150        160        170        180 
VKANEGTVGV SAATQRTQCN TTQGNEVTSI LRWAKDSGKS VGIVTTTRVN HATPSAAYAH 

       190        200        210        220        230        240 
SADRDWYSDN EMPPEALSQG CKDIAYQLMH NVRDIEVIMG GGRKYMFPKN RTDVEYEMDE 

       250        260        270        280        290        300 
KARGTRLDGL NLVDIWKSFK PRHKHSHYVW NRTELLTLDP YGVDYLLGLF EPGDMQYELN 

       310        320        330        340        350        360 
RNSTTDPSLS EMVEIAIKIL SKNPKGFFLL VEGGRIDHGH HEGKAKQALH EAVEMDQAIG 

       370        380        390        400        410        420 
RAGAMTSVED TLTIVTADHS HVFTFGGYTP RGNSIFGLAP MVSDTDKKPF TSILYGNGPG 

       430        440        450        460        470        480 
YKVVGGEREN VSMVDYAHNN YQAQSAVPLR HETHGGEDVA VFAKGPMAHL LHGVHEQNYI 

       490        500        510        520 
PHVMAYAACI GANLDHCASA SSAGGPSPGP LFLLLALPSL GILF

« Hide

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References

[1]"cDNA encoding a functional feline liver/bone/kidney-type alkaline phosphatase."
Ghosh A.K., Mullins J.I.
Arch. Biochem. Biophys. 322:240-249(1995) [PubMed: 7574682] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U31569 mRNA. Translation: AAA82993.1.
PIRS66467.
RefSeqNP_001036028.1.

3D structure databases

SMRQ29486. Positions 18-498.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ29486.

Genome annotation databases

EnsemblENSFCAT00000002960; ENSFCAP00000002729; ENSFCAG00000002960; Felis catus. [Genome view]
GeneID727689.

Organism-specific databases

CTD727689.

Phylogenomic databases

eggNOGmaNOG13999.
HOVERGENQ29486.

Enzyme and pathway databases

BRENDA3.1.3.1. 273949.

Family and domain databases

InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
Gene3DG3DSA:3.40.720.10. Alk_phosphtse. 1 hit.
PfamPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSPR00113. ALKPHPHTASE.
SMARTSM00098. alkPPc. 1 hit.
[Graphical view]
PROSITEPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePPBT_FELCA
AccessionPrimary (citable) accession number: Q29486
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: February 9, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents