Reviewed,
UniProtKB/Swiss-Prot Q29486 (PPBT_FELCA)
Last modified
February 9, 2010.
Version 65.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
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Names and origin
| Protein names | Recommended name: Alkaline phosphatase, tissue-nonspecific isozyme Short name=AP-TNAP Short name=TNSALP EC=3.1.3.1 Alternative name(s): Alkaline phosphatase liver/bone/kidney isozyme | ||
| Gene names |
| ||
| Organism | Felis catus (Cat) (Felis silvestris catus) | ||
| Taxonomic identifier | 9685 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Feliformia › Felidae › Felinae › Felis |
Protein attributes
| Sequence length | 524 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Catalytic activity | A phosphate monoester + H2O = an alcohol + phosphate. |
| Cofactor | Binds 1 magnesium ion By similarity. Binds 2 zinc ions By similarity. |
| Subunit structure | Homodimer By similarity. |
| Subcellular location | Cell membrane; Lipid-anchor › GPI-anchor By similarity. |
| Miscellaneous | In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). |
| Sequence similarities | Belongs to the alkaline phosphatase family. |
Ontologies
| Keywords | |
|---|---|
| Cellular component | Cell membrane Membrane |
| Domain | Signal |
| Ligand | Magnesium Metal-binding Zinc |
| Molecular function | Hydrolase |
| PTM | GPI-anchor Glycoprotein Lipoprotein Phosphoprotein |
| Technical term | Direct protein sequencing |
| Gene Ontology (GO) | |
| Biological process | metabolic process Inferred from electronic annotation. Source: InterPro |
| Cellular component | anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membraneInferred from electronic annotation. Source: UniProtKB-KW |
| Molecular function | alkaline phosphatase activity Inferred from electronic annotation. Source: EC magnesium ion bindingInferred from electronic annotation. Source: UniProtKB-KW zinc ion bindingInferred from electronic annotation. Source: UniProtKB-KW |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Signal peptide | 1 – 17 | 17 | |||||||
| Chain | 18 – 502 | 485 | Alkaline phosphatase, tissue-nonspecific isozyme | PRO_0000024021 | |||||
| Propeptide | 503 – 524 | 22 | Removed in mature form Potential | PRO_0000024022 | |||||
Sites | |||||||||
| Active site | 110 | 1 | Phosphoserine intermediate By similarity | ||||||
| Metal binding | 60 | 1 | Magnesium Potential | ||||||
| Metal binding | 60 | 1 | Zinc 2 Potential | ||||||
| Metal binding | 173 | 1 | Magnesium Potential | ||||||
| Metal binding | 332 | 1 | Magnesium Potential | ||||||
| Metal binding | 337 | 1 | Zinc 1 Potential | ||||||
| Metal binding | 341 | 1 | Zinc 1 Potential | ||||||
| Metal binding | 378 | 1 | Zinc 2 Potential | ||||||
| Metal binding | 379 | 1 | Zinc 2 Potential | ||||||
| Metal binding | 454 | 1 | Zinc 1 Potential | ||||||
Amino acid modifications | |||||||||
| Lipidation | 502 | 1 | GPI-anchor amidated serine Potential | ||||||
| Glycosylation | 140 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 230 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 271 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 302 | 1 | N-linked (GlcNAc...) Potential | ||||||
| Glycosylation | 430 | 1 | N-linked (GlcNAc...) Potential | ||||||
Sequences
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References
| [1] | "cDNA encoding a functional feline liver/bone/kidney-type alkaline phosphatase." Ghosh A.K., Mullins J.I. Arch. Biochem. Biophys. 322:240-249(1995) [PubMed: 7574682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | U31569 mRNA. Translation: AAA82993.1. |
| PIR | S66467. |
| RefSeq | NP_001036028.1. |
3D structure databases | |
| SMR | Q29486. Positions 18-498. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q29486. |
Genome annotation databases | |
| Ensembl | ENSFCAT00000002960; ENSFCAP00000002729; ENSFCAG00000002960; Felis catus. [Genome view] |
| GeneID | 727689. |
Organism-specific databases | |
| CTD | 727689. |
Phylogenomic databases | |
| eggNOG | maNOG13999. |
| HOVERGEN | Q29486. |
Enzyme and pathway databases | |
| BRENDA | 3.1.3.1. 273949. |
Family and domain databases | |
| InterPro | IPR017849. Alkaline_Pase-like_a/b/a. IPR001952. Alkaline_phosphatase. IPR018299. Alkaline_phosphatase_AS. IPR017850. Alkaline_phosphatase_core. [Graphical view] |
| Gene3D | G3DSA:3.40.720.10. Alk_phosphtse. 1 hit. |
| Pfam | PF00245. Alk_phosphatase. 1 hit. [Graphical view] |
| PRINTS | PR00113. ALKPHPHTASE. |
| SMART | SM00098. alkPPc. 1 hit. [Graphical view] |
| PROSITE | PS00123. ALKALINE_PHOSPHATASE. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | PPBT_FELCA | ||||||||
| Accession | Primary (citable) accession number: Q29486 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

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