Q29473 (CP2DF_CANFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 2D15
EC=1.14.14.1

Alternative name(s):
CYPIID15
Cytochrome P450 DUT2

Gene names

Name:

CYP2D15

Organism

Canis familiaris (Dog) (Canis lupus familiaris) [Reference proteome]

Taxonomic identifier

9615 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis › Canis lupus

Protein attributes

Sequence length	500 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	High activity for the hydroxylation of bunitrolol and imipramine; low activity on debrisoquine.
Catalytic activity	RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.
Cofactor	Heme group By similarity.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Tissue specificity	Liver. Also detected in several other tissues.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aromatase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 500	499	Cytochrome P450 2D15		PRO_0000051739
Sites
	Metal binding	446	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q29473 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: E7E352A01FABAED5	FASTA	500	56,432
	10 20 30 40 50 60 MGLLTGDTLG PLAVAVAIFL LLVDLMHRRR RWATRYPPGP TPVPMVGNLL QMDFQEPICY 70 80 90 100 110 120 FSQLQGRFGN VFSLELAWTP VVVLNGLEAV REALVHRSED TADRPPMPIY DHLGLGPESQ 130 140 150 160 170 180 GLFLARYGRA WREQRRFSLS TLRNFGLGRK SLEQWVTEEA SCLCAAFAEQ AGRPFGPGAL 190 200 210 220 230 240 LNKAVSNVIS SLTYGRRFEY DDPRLLQLLE LTQQALKQDS GFLREALNSI PVLLHIPGLA 250 260 270 280 290 300 SKVFSAQKAI ITLTNEMIQE HRKTRDPTQP PRHLIDAFVD EIEKAKGNPK TSFNEENLCM 310 320 330 340 350 360 VTSDLFIAGM VSTSITLTWA LLLMILHPDV QRRVQQEIDE VIGREQLPEM GDQTRMPFTV 370 380 390 400 410 420 AVIHEVQRFG DIVPLGVPHM TSRDTEVQGF LIPKGTTLIT NLSSVLKDEK VWKKPFRFYP 430 440 450 460 470 480 EHFLDAQGHF VKHEAFMPFS AGRRVCLGEP LARMELFLFF TCLLQRFSFS VPAGQPRPSD 490 500 HGVFTFLKVP APFQLCVEPR

References

[1]	"A new cytochrome P450 form belonging to the CYP2D in dog liver microsomes: purification, cDNA cloning, and enzyme characterization." Sakamoto K., Kirita S., Baba T., Nakamura Y., Yamazoe Y., Kato R., Takanaka A., Matsubara T. Arch. Biochem. Biophys. 319:372-382(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
[2]	"Expression and characterization of dog CYP2D15 using baculovirus expression system." Tasaki T., Nakamura A., Itoh S., Ohashi K., Yamamoto Y., Masuda M., Iwata H., Kazusaka A., Kamataki T., Fujita S. J. Biochem. 123:162-168(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Beagle.
[3]	"Expression and characterization of canine cytochrome P450 2D15." Roussel F., Duignan D.B., Lawton M.P., Obach R.S., Strick C.A., Tweedie D.J. Arch. Biochem. Biophys. 357:27-36(1998) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D17397 mRNA. Translation: BAA04220.1. AB004268 mRNA. Translation: BAA20357.1.
PIR	JC4157.
RefSeq	NP_001003333.1. NM_001003333.1.
UniGene	Cfa.15290.
3D structure databases
ProteinModelPortal	Q29473.
SMR	Q29473. Positions 37-500.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	415120.
KEGG	cfa:415120.
Organism-specific databases
CTD	415120.
Phylogenomic databases
HOVERGEN	HBG015789.
KO	K07414.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. IPR008069. Cyt_P450_E_grp-I_CYP2D-like. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR01686. EP450ICYP2D. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChEMBL	CHEMBL1795137.
NextBio	20818799.

Entry information

Entry name

CP2DF_CANFA

Accession

Primary (citable) accession number: Q29473
Secondary accession number(s): O02859

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 91 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program