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Protein

Cytochrome P450 2D15

Gene

CYP2D15

Organism
Canis familiaris (Dog) (Canis lupus familiaris)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

High activity for the hydroxylation of bunitrolol and imipramine; low activity on debrisoquine.

Catalytic activityi

RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O.

Cofactori

hemeBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi446 – 4461Iron (heme axial ligand)By similarity

GO - Molecular functioni

  1. aromatase activity Source: UniProtKB-EC
  2. heme binding Source: InterPro
  3. iron ion binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome P450 2D15 (EC:1.14.14.1)
Alternative name(s):
CYPIID15
Cytochrome P450 DUT2
Gene namesi
Name:CYP2D15
OrganismiCanis familiaris (Dog) (Canis lupus familiaris)
Taxonomic identifieri9615 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCarnivoraCaniformiaCanidaeCanis
ProteomesiUP000002254 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500Cytochrome P450 2D15PRO_0000051739Add
BLAST

Expressioni

Tissue specificityi

Liver. Also detected in several other tissues.

Structurei

3D structure databases

ProteinModelPortaliQ29473.
SMRiQ29473. Positions 37-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

HOVERGENiHBG015789.
InParanoidiQ29473.
KOiK07414.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01686. EP450ICYP2D.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q29473-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLLTGDTLG PLAVAVAIFL LLVDLMHRRR RWATRYPPGP TPVPMVGNLL
60 70 80 90 100
QMDFQEPICY FSQLQGRFGN VFSLELAWTP VVVLNGLEAV REALVHRSED
110 120 130 140 150
TADRPPMPIY DHLGLGPESQ GLFLARYGRA WREQRRFSLS TLRNFGLGRK
160 170 180 190 200
SLEQWVTEEA SCLCAAFAEQ AGRPFGPGAL LNKAVSNVIS SLTYGRRFEY
210 220 230 240 250
DDPRLLQLLE LTQQALKQDS GFLREALNSI PVLLHIPGLA SKVFSAQKAI
260 270 280 290 300
ITLTNEMIQE HRKTRDPTQP PRHLIDAFVD EIEKAKGNPK TSFNEENLCM
310 320 330 340 350
VTSDLFIAGM VSTSITLTWA LLLMILHPDV QRRVQQEIDE VIGREQLPEM
360 370 380 390 400
GDQTRMPFTV AVIHEVQRFG DIVPLGVPHM TSRDTEVQGF LIPKGTTLIT
410 420 430 440 450
NLSSVLKDEK VWKKPFRFYP EHFLDAQGHF VKHEAFMPFS AGRRVCLGEP
460 470 480 490 500
LARMELFLFF TCLLQRFSFS VPAGQPRPSD HGVFTFLKVP APFQLCVEPR
Length:500
Mass (Da):56,432
Last modified:January 23, 2007 - v3
Checksum:iE7E352A01FABAED5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17397 mRNA. Translation: BAA04220.1.
AB004268 mRNA. Translation: BAA20357.1.
PIRiJC4157.
RefSeqiNP_001003333.1. NM_001003333.1.
UniGeneiCfa.15290.

Genome annotation databases

GeneIDi415120.
KEGGicfa:415120.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17397 mRNA. Translation: BAA04220.1.
AB004268 mRNA. Translation: BAA20357.1.
PIRiJC4157.
RefSeqiNP_001003333.1. NM_001003333.1.
UniGeneiCfa.15290.

3D structure databases

ProteinModelPortaliQ29473.
SMRiQ29473. Positions 37-500.
ModBaseiSearch...
MobiDBiSearch...

Chemistry

ChEMBLiCHEMBL1795137.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi415120.
KEGGicfa:415120.

Organism-specific databases

CTDi415120.

Phylogenomic databases

HOVERGENiHBG015789.
InParanoidiQ29473.
KOiK07414.

Miscellaneous databases

NextBioi20818799.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
IPR008069. Cyt_P450_E_grp-I_CYP2D-like.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR01686. EP450ICYP2D.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "A new cytochrome P450 form belonging to the CYP2D in dog liver microsomes: purification, cDNA cloning, and enzyme characterization."
    Sakamoto K., Kirita S., Baba T., Nakamura Y., Yamazoe Y., Kato R., Takanaka A., Matsubara T.
    Arch. Biochem. Biophys. 319:372-382(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Liver.
  2. "Expression and characterization of dog CYP2D15 using baculovirus expression system."
    Tasaki T., Nakamura A., Itoh S., Ohashi K., Yamamoto Y., Masuda M., Iwata H., Kazusaka A., Kamataki T., Fujita S.
    J. Biochem. 123:162-168(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Beagle.
  3. Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiCP2DF_CANFA
AccessioniPrimary (citable) accession number: Q29473
Secondary accession number(s): O02859
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: January 23, 2007
Last modified: January 7, 2015
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.