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Q29463 (TRY2_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Anionic trypsin

EC=3.4.21.4
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length247 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Cofactor

Binds 1 calcium ion per subunit By similarity.

Subcellular location

Secretedextracellular space.

Post-translational modification

Not sulfated on tyrosine residue(s).

Sequence similarities

Belongs to the peptidase S1 family.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1515 Potential
Propeptide16 – 238Activation peptide
PRO_0000028189
Chain24 – 247224Anionic trypsin
PRO_0000028190

Regions

Domain24 – 244221Peptidase S1

Sites

Active site631Charge relay system
Active site1071Charge relay system
Active site2001Charge relay system
Metal binding751Calcium By similarity
Metal binding771Calcium; via carbonyl oxygen By similarity
Metal binding801Calcium; via carbonyl oxygen By similarity
Metal binding851Calcium By similarity
Site1941Required for specificity

Amino acid modifications

Disulfide bond30 ↔ 160 By similarity
Disulfide bond48 ↔ 64 By similarity
Disulfide bond132 ↔ 233 By similarity
Disulfide bond139 ↔ 206 By similarity
Disulfide bond171 ↔ 185 By similarity
Disulfide bond196 ↔ 220 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q29463 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 50A070495A7731DB

FASTA24726,290
        10         20         30         40         50         60 
MHPLLILAFV GAAVAFPSDD DDKIVGGYTC AENSVPYQVS LNAGYHFCGG SLINDQWVVS 

        70         80         90        100        110        120 
AAHCYQYHIQ VRLGEYNIDV LEGGEQFIDA SKIIRHPKYS SWTLDNDILL IKLSTPAVIN 

       130        140        150        160        170        180 
ARVSTLLLPS ACASAGTECL ISGWGNTLSS GVNYPDLLQC LVAPLLSHAD CEASYPGQIT 

       190        200        210        220        230        240 
NNMICAGFLE GGKDSCQGDS GGPVACNGQL QGIVSWGYGC AQKGKPGVYT KVCNYVDWIQ 


ETIAANS 

« Hide

References

[1]"Isolation and nucleotide sequence of cDNA clone for bovine pancreatic anionic trypsinogen. Structural identity within the trypsin family."
le Huerou I., Wicker C., Guilloteau P., Toullec R., Puigserver A.
Eur. J. Biochem. 193:767-773(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Holstein-Friesian.
Tissue: Pancreas.
[2]"Human cationic trypsinogen is sulfated on Tyr154."
Sahin-Toth M., Kukor Z., Nemoda Z.
FEBS J. 273:5044-5050(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: ABSENCE OF SULFATED TYROSINE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X54703 mRNA. Translation: CAA38513.1.
PIRS13813.
UniGeneBt.1978.

3D structure databases

ProteinModelPortalQ29463.
SMRQ29463. Positions 24-246.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000028731.

Chemistry

BindingDBQ29463.
ChEMBLCHEMBL4472.

Protein family/group databases

MEROPSS01.120.

Proteomic databases

PRIDEQ29463.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

eggNOGCOG5640.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidQ29463.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRY2_BOVIN
AccessionPrimary (citable) accession number: Q29463
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: April 16, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries