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Q29463

- TRY2_BOVIN

UniProt

Q29463 - TRY2_BOVIN

Protein

Anionic trypsin

Gene
N/A
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

    Cofactori

    Binds 1 calcium ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei63 – 631Charge relay system
    Metal bindingi75 – 751CalciumBy similarity
    Metal bindingi77 – 771Calcium; via carbonyl oxygenBy similarity
    Metal bindingi80 – 801Calcium; via carbonyl oxygenBy similarity
    Metal bindingi85 – 851CalciumBy similarity
    Active sitei107 – 1071Charge relay system
    Sitei194 – 1941Required for specificity
    Active sitei200 – 2001Charge relay system

    GO - Molecular functioni

    1. calcium ion binding Source: UniProtKB
    2. serine-type endopeptidase activity Source: UniProtKB

    GO - Biological processi

    1. collagen catabolic process Source: UniProtKB
    2. digestion Source: UniProtKB-KW
    3. proteolysis Source: UniProtKB

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Keywords - Biological processi

    Digestion

    Keywords - Ligandi

    Calcium, Metal-binding

    Protein family/group databases

    MEROPSiS01.120.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anionic trypsin (EC:3.4.21.4)
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB
    2. extracellular space Source: UniProtKB

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1515Sequence AnalysisAdd
    BLAST
    Propeptidei16 – 238Activation peptidePRO_0000028189
    Chaini24 – 247224Anionic trypsinPRO_0000028190Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi30 ↔ 160PROSITE-ProRule annotation
    Disulfide bondi48 ↔ 64PROSITE-ProRule annotation
    Disulfide bondi132 ↔ 233PROSITE-ProRule annotation
    Disulfide bondi139 ↔ 206PROSITE-ProRule annotation
    Disulfide bondi171 ↔ 185PROSITE-ProRule annotation
    Disulfide bondi196 ↔ 220PROSITE-ProRule annotation

    Post-translational modificationi

    Not sulfated on tyrosine residue(s).

    Keywords - PTMi

    Disulfide bond, Zymogen

    Proteomic databases

    PRIDEiQ29463.

    Interactioni

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000028731.

    Structurei

    3D structure databases

    ProteinModelPortaliQ29463.
    SMRiQ29463. Positions 24-246.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 244221Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiQ29463.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q29463-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHPLLILAFV GAAVAFPSDD DDKIVGGYTC AENSVPYQVS LNAGYHFCGG    50
    SLINDQWVVS AAHCYQYHIQ VRLGEYNIDV LEGGEQFIDA SKIIRHPKYS 100
    SWTLDNDILL IKLSTPAVIN ARVSTLLLPS ACASAGTECL ISGWGNTLSS 150
    GVNYPDLLQC LVAPLLSHAD CEASYPGQIT NNMICAGFLE GGKDSCQGDS 200
    GGPVACNGQL QGIVSWGYGC AQKGKPGVYT KVCNYVDWIQ ETIAANS 247
    Length:247
    Mass (Da):26,290
    Last modified:November 1, 1996 - v1
    Checksum:i50A070495A7731DB
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54703 mRNA. Translation: CAA38513.1.
    PIRiS13813.
    UniGeneiBt.1978.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X54703 mRNA. Translation: CAA38513.1 .
    PIRi S13813.
    UniGenei Bt.1978.

    3D structure databases

    ProteinModelPortali Q29463.
    SMRi Q29463. Positions 24-246.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000028731.

    Chemistry

    BindingDBi Q29463.
    ChEMBLi CHEMBL4472.

    Protein family/group databases

    MEROPSi S01.120.

    Proteomic databases

    PRIDEi Q29463.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    eggNOGi COG5640.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi Q29463.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and nucleotide sequence of cDNA clone for bovine pancreatic anionic trypsinogen. Structural identity within the trypsin family."
      le Huerou I., Wicker C., Guilloteau P., Toullec R., Puigserver A.
      Eur. J. Biochem. 193:767-773(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Holstein-Friesian.
      Tissue: Pancreas.
    2. "Human cationic trypsinogen is sulfated on Tyr154."
      Sahin-Toth M., Kukor Z., Nemoda Z.
      FEBS J. 273:5044-5050(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: ABSENCE OF SULFATED TYROSINE.

    Entry informationi

    Entry nameiTRY2_BOVIN
    AccessioniPrimary (citable) accession number: Q29463
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3