Reviewed, UniProtKB/Swiss-Prot Q29463 (TRY2_BOVIN)

Names and origin

Protein names	Recommended name:     Anionic trypsin     EC=3.4.21.4
Organism	Bos taurus (Bovine)
Taxonomic identifier	9913 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	247 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.
Cofactor	Binds 1 calcium ion per subunit By similarity.
Subcellular location	Secreted › extracellular space.
Post-translational modification	Not sulfated on tyrosine residue(s).
Sequence similarities	Belongs to the peptidase S1 family. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Biological process	Digestion
Cellular component	Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	collagen catabolic process Inferred from sequence or structural similarity. Source: UniProtKB digestion Inferred from electronic annotation. Source: UniProtKB-KW proteolysis involved in cellular protein catabolic process Inferred from direct assay. Source: MGI
Cellular component	extracellular space Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	calcium ion binding Inferred from sequence or structural similarity. Source: UniProtKB protein binding Inferred from sequence or structural similarity. Source: UniProtKB serine-type endopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 15	15	Potential
	Propeptide	16 – 23	8	Activation peptide		PRO_0000028189
	Chain	24 – 247	224	Anionic trypsin		PRO_0000028190
Regions
	Domain	24 – 244	221	Peptidase S1
Sites
	Active site	63	1	Charge relay system
	Active site	107	1	Charge relay system
	Active site	200	1	Charge relay system
	Metal binding	75	1	Calcium By similarity
	Metal binding	77	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	80	1	Calcium; via carbonyl oxygen By similarity
	Metal binding	85	1	Calcium By similarity
	Site	194	1	Required for specificity
Amino acid modifications
	Disulfide bond	30 ↔ 160		By similarity
	Disulfide bond	48 ↔ 64		By similarity
	Disulfide bond	132 ↔ 233		By similarity
	Disulfide bond	139 ↔ 206		By similarity
	Disulfide bond	171 ↔ 185		By similarity
	Disulfide bond	196 ↔ 220		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q29463-1 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: 50A070495A7731DB	FASTA	247	26,290
	10 20 30 40 50 60 MHPLLILAFV GAAVAFPSDD DDKIVGGYTC AENSVPYQVS LNAGYHFCGG SLINDQWVVS 70 80 90 100 110 120 AAHCYQYHIQ VRLGEYNIDV LEGGEQFIDA SKIIRHPKYS SWTLDNDILL IKLSTPAVIN 130 140 150 160 170 180 ARVSTLLLPS ACASAGTECL ISGWGNTLSS GVNYPDLLQC LVAPLLSHAD CEASYPGQIT 190 200 210 220 230 240 NNMICAGFLE GGKDSCQGDS GGPVACNGQL QGIVSWGYGC AQKGKPGVYT KVCNYVDWIQ ETIAANS

References

[1]	"Isolation and nucleotide sequence of cDNA clone for bovine pancreatic anionic trypsinogen. Structural identity within the trypsin family." le Huerou I., Wicker C., Guilloteau P., Toullec R., Puigserver A. Eur. J. Biochem. 193:767-773(1990) [PubMed: 1701147] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Holstein-Friesian. Tissue: Pancreas.
[2]	"Human cationic trypsinogen is sulfated on Tyr154." Sahin-Toth M., Kukor Z., Nemoda Z. FEBS J. 273:5044-5050(2006) [PubMed: 17087724] [Abstract] Cited for: ABSENCE OF SULFATED TYROSINE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X54703 mRNA. Translation: CAA38513.1.
IPI	IPI00685911.
PIR	S13813.
UniGene	Bt.1978
3D structure databases
HSSP	HSSP built from PDB template 1EZX based on UniProtKB P00760.
SMR	Q29463. Positions 24-246.
ModBase	Search...
Protein family/group databases
MEROPS	S01.120.
Genome annotation databases
Ensembl	ENSBTAG00000021565. Bos taurus. [Contig view]
Phylogenomic databases
HOVERGEN	Q29463.
Enzyme and pathway databases
BRENDA	3.4.21.4. 251.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRY2_BOVIN

Accession

Primary (citable) accession number: Q29463

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1996
Last modified:	May 5, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)