Reviewed, UniProtKB/Swiss-Prot Q29461 (ELA2A_BOVIN)

Names and origin

Protein names

Recommended name:
    Elastase-2A
    EC=3.4.21.71
Alternative name(s):
    Elastase-2
    Elastase II

Gene names

Name:	ELA2A
Synonyms:	ELA2

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Acts upon elastin.
Catalytic activity	Preferential cleavage: Leu-|-Xaa, Met-|-Xaa and Phe-|-Xaa. Hydrolyzes elastin.
Subcellular location	Secreted.
Tissue specificity	Pancreas.
Sequence similarities	Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	By similarity
	Propeptide	17 – 28	12	Activation peptide By similarity		PRO_0000027685
	Chain	29 – 269	241	Elastase-2A		PRO_0000027686
Regions
	Domain	29 – 267	239	Peptidase S1
Sites
	Active site	73	1	Charge relay system By similarity
	Active site	121	1	Charge relay system By similarity
	Active site	216	1	Charge relay system By similarity
Amino acid modifications
	Disulfide bond	58 ↔ 74		By similarity
	Disulfide bond	155 ↔ 222		By similarity
	Disulfide bond	186 ↔ 202		By similarity
	Disulfide bond	212 ↔ 243		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q29461-1 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 8343B97062CF267C	FASTA	269	28,857
	10 20 30 40 50 60 MIRALLLSTL VAGALSCGVP TYPPQLSRVV GGEDARPNSW PWQVSLQYSS SGQWRHTCGG 70 80 90 100 110 120 SLIEQNWVLT AAHCISSSRT YRVVVGRQSL STVESGSLTI AVSKSVIHEK WNSNQLAQGN 130 140 150 160 170 180 DIALLKLASS VPLTDKIQLG CLPAAGTILP NNYVCYVTGW GRLQSNGALP DILQQGKLLV 190 200 210 220 230 240 VDYATCSNPS WWGSTVKTNM ICAGGDGVTS SCNGDSGGPL NCQAANRQWQ VHGIVSFGSS 250 260 LGCNYYRKPS VFTRVSNYND WISSVIENN

References

[1]

"Bovine pancreatic preproelastases I and II: comparison of nucleotide and amino acid sequences and tissue specific expression." Gestin M., le Huerou-Luron I., Wicker-Planquart C., le Drean G., Chaix J.-C., Puigserver A., Guilloteau P. Comp. Biochem. Physiol. 118B:181-187(1997) [PubMed: 9418008] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Pancreas.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X97635 mRNA. Translation: CAA66231.1.
IPI	IPI00687568.
RefSeq	NP_777139.1.
UniGene	Bt.311
3D structure databases
HSSP	HSSP built from PDB template 1BRU based on UniProtKB P08419.
SMR	Q29461. Positions 29-269.
ModBase	Search...
Protein family/group databases
MEROPS	S01.155.
Genome annotation databases
Ensembl	ENSBTAG00000024496. Bos taurus. [Contig view]
GeneID	282687.
KEGG	bta:282687.
Phylogenomic databases
HOVERGEN	Q29461.
Enzyme and pathway databases
BRENDA	3.4.21.71. 251.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ELA2A_BOVIN

Accession

Primary (citable) accession number: Q29461

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)