Platelet-activating factor acetylhydrolase IB subunit gamma

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Q29460 (PA1B3_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 96. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Platelet-activating factor acetylhydrolase IB subunit gamma
EC=3.1.1.47

Alternative name(s):
PAF acetylhydrolase 29 kDa subunit
Short name=PAF-AH 29 kDa subunit
PAF-AH subunit gamma
Short name=PAFAH subunit gamma

Gene names

Name:	PAFAH1B3
Synonyms:	PAFAHG

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	232 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Inactivates paf by removing the acetyl group at the sn-2 position. This is a catalytic subunit. Plays an important role during the development of brain.
Catalytic activity	1-alkyl-2-acetyl-sn-glycero-3-phosphocholine + H₂O = 1-alkyl-sn-glycero-3-phosphocholine + acetate.
Subunit structure	Cytosolic PAF-AH IB is formed of three subunits of 45 kDa (alpha), 30 kDa (beta) and 29 kDa (gamma). The catalytic activity of the enzyme resides in the beta and gamma subunits, whereas the alpha subunit has regulatory activity. Trimer formation is not essential for the catalytic activity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the 'GDSL' lipolytic enzyme family. Platelet-activating factor acetylhydrolase IB beta/gamma subunits subfamily.

Ontologies

Keywords
Biological process	Lipid degradation Lipid metabolism
Cellular component	Cytoplasm
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW spermatogenesis Inferred from sequence or structural similarity. Source: AgBase
Cellular_component	cytoplasm Inferred from sequence or structural similarity. Source: AgBase
Molecular_function	1-alkyl-2-acetylglycerophosphocholine esterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 232

232

Platelet-activating factor acetylhydrolase IB subunit gamma

PRO_0000058154

Sites

Active site

192

Active site

195

Experimental info

Sequence conflict

Missing AA sequence Ref.1

Sequence conflict

150

E → L AA sequence Ref.1

Secondary structure

232

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q29460 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 81C2CECA8E2204ED
Show »

FASTA

232

25,865

        10         20         30         40         50         60 
MSGDENPASK PTPVQDVQGD GRWMSLHHRF VADSKDKEPE VVFIGDSLVQ LMHQCEIWRE 

        70         80         90        100        110        120 
LFSPLHALNF GIGGDSTQHV LWRLENGELE HIRPKIVVVW VGTNNHGHTA EQVTGGIKAI 

       130        140        150        160        170        180 
VQLVNERQPQ ARVVVLGLLP RGQHPNPLRE KNRRVNELVR AALAGHPRAH FLDADPGFVH 

       190        200        210        220        230 
SDGTISHHDM YDYLHLSRLG YTPVCRALHS LLLRLLTQDQ GQGGAPLPEP SP

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The catalytic subunit of bovine brain platelet-activating factor acetylhydrolase is a novel type of serine esterase." Hattori M., Adachi H., Tsujimoto M., Arai H., Inoue K. J. Biol. Chem. 269:23150-23155(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 38-85; 96-111 AND 119-151. Tissue: Brain.
[2]	NIH - Mammalian Gene Collection (MGC) project Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Hereford. Tissue: Fetal brain.
[3]	"Brain acetylhydrolase that inactivates platelet-activating factor is a G-protein-like trimer." Ho Y.S., Swenson L., Derewenda U., Serre L., Wei Y., Dauter Z., Hattori M., Adachi T., Aoki J., Arai H., Inoue K., Derewenda Z.S. Nature 385:89-93(1997) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D30789 mRNA. Translation: BAA06455.1.
BC120202 mRNA. Translation: AAI20203.1.

IPI

IPI00715508.

PIR

A54754.

RefSeq

NP_777090.1. NM_174665.2.

UniGene

Bt.4040.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BWP	X-ray	2.10	A	1-232	[»]
1BWQ	X-ray	2.30	A	1-232	[»]
1BWR	X-ray	2.40	A	1-232	[»]
1ES9	X-ray	1.30	A	1-232	[»]
1FXW	X-ray	2.10	A	1-232	[»]
1WAB	X-ray	1.70	A	1-232	[»]
3DT6	X-ray	2.10	A	1-232	[»]
3DT8	X-ray	1.85	A	1-232	[»]
3DT9	X-ray	1.85	A	1-232	[»]

ProteinModelPortal

Q29460.

SMR

Q29460. Positions 5-215.

ModBase

Search...

Protein-protein interaction databases

IntAct

Q29460. 1 interaction.

MINT

MINT-208804.

Proteomic databases

PaxDb

Q29460.

PRIDE

Q29460.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSBTAT00000026366; ENSBTAP00000026366; ENSBTAG00000019787.

GeneID

282515.

KEGG

bta:282515.

Organism-specific databases

CTD

5050.

Phylogenomic databases

eggNOG

NOG69837.

GeneTree

ENSGT00390000016520.

HOGENOM

HOG000232143.

HOVERGEN

HBG053477.

InParanoid

Q29460.

K16795.

OMA

FAPLHCL.

OrthoDB

EOG45QHF4.

Family and domain databases

Gene3D

3.40.50.1110. 1 hit.

InterPro

IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]

PROSITE

PS01098. LIPASE_GDSL_SER. False negative.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

Q29460.

NextBio

20806265.

Entry information

Entry name

PA1B3_BOVIN

Accession

Primary (citable) accession number: Q29460
Secondary accession number(s): Q0VCF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	November 1, 1996
Last modified:	April 3, 2013
This is version 96 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Sites

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Family and domain databases

Other

Entry information

Relevant documents