Q29458 (LIPG_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gastric triacylglycerol lipase
Short name=GL
Short name=Gastric lipase
EC=3.1.1.3

Alternative name(s):
Pregastric esterase
Short name=PGE

Gene names

Name:

LIPF

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Secreted.
Miscellaneous	Not inhibited by the thiol reagents 5,5'-dithiobis(2-nitrobenzoic acid) or 4,4'-dithiopyridine.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	lipid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	By similarity
	Chain	20 – 397	378	Gastric triacylglycerol lipase		PRO_0000017764
Sites
	Active site	171	1	Nucleophile By similarity
	Active site	342	1	Charge relay system By similarity
	Active site	371	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	33	1	N-linked (GlcNAc...) Potential
	Glycosylation	270	1	N-linked (GlcNAc...) Potential
	Glycosylation	326	1	N-linked (GlcNAc...) Potential
	Disulfide bond	245 ↔ 254		Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q29458 [UniParc]. Last modified November 1, 1996. Version 1. Checksum: F68977DED585EE36	FASTA	397	45,231
	10 20 30 40 50 60 MWWLLVTVCF IHMSGNAFCF LGKIAKNPEA SMNVSQMISY WGYPSEMHKV ITADGYILQV 70 80 90 100 110 120 YRIPHGKNNA NHLGQRPVVF LQHGLLGSAT NWISNLPKNS LGFLLADAGY DVWLGNSRGN 130 140 150 160 170 180 TWAQEHLYYS PDSPEFWAFS FDEMAEYDLP STIDFILRRT GQKKLHYVGH SQGTTIGFIA 190 200 210 220 230 240 FSTSPTLAEK IKVFYALAPV ATVKYTKSLF NKLALIPHFL FKIIFGDKMF YPHTFLEQFL 250 260 270 280 290 300 GVEMCSRETL DVLCKNALFA ITGVDNKNFN MSRLDVYIAH NPAGTSVQNT LHWRQAVKSG 310 320 330 340 350 360 KFQAFDWGAP YQNLMHYHQP TPPIYNLTAM NVPIAVWSAD NDLLADPQDV DFLLSKLSNL 370 380 390 IYHKEIPNYN HLDFIWAMDA PQEVYNEIVS LMAEDKK

References

[1]	"The cDNA sequence encoding bovine pregastric esterase." Timmermans M.Y.J., Kupers L.P.M., Teuchy H. Gene 147:259-262(1994) [PubMed: 7926811] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Tongue serous gland.
[2]	"Inhibition studies on calf pregastric esterase: the enzyme has no functional thiol group." Timmermans M.Y.J., Reekmans G., Teuchy H.J.H., Kupers L.P.M. Biochem. J. 314:931-936(1996) [PubMed: 8615791] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, DISULFIDE BONDS.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L26319 mRNA. Translation: AAA57037.1.
IPI	IPI00706693.
PIR	JC4017.
RefSeq	NP_776528.1. NM_174103.2.
UniGene	Bt.503.
3D structure databases
ProteinModelPortal	Q29458.
SMR	Q29458. Positions 27-395.
ModBase	Search...
Protein-protein interaction databases
STRING	Q29458.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	281283.
KEGG	bta:281283.
Organism-specific databases
CTD	8513.
Phylogenomic databases
eggNOG	maNOG18630.
GeneTree	ENSGT00550000074328.
HOVERGEN	HBG006265.
InParanoid	Q29458.
OrthoDB	EOG43TZVJ.
PhylomeDB	Q29458.
Family and domain databases
InterPro	IPR000073. AB_hydrolase_1. IPR006693. AB_hydrolase_lipase. [Graphical view]
KO	K14452.
Pfam	PF04083. Abhydro_lipase. 1 hit. PF00561. Abhydrolase_1. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LIPG_BOVIN

Accession

Primary (citable) accession number: Q29458

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 1, 1996
Last modified:	November 16, 2011
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program