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Protein

Probable phospholipid-transporting ATPase IA

Gene

ATP8A1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in the transport of aminophospholipids from the outer to the inner leaflet of various membranes and the maintenance of asymmetric distribution of phospholipids, mainly in secretory vesicles.

Catalytic activityi

ATP + H2O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei409 – 40914-aspartylphosphate intermediateBy similarity
Metal bindingi786 – 7861MagnesiumBy similarity
Metal bindingi790 – 7901MagnesiumBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi726 – 7338ATPSequence analysis
Nucleotide bindingi1080 – 10878ATPSequence analysis

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi3.6.3.1. 908.

Protein family/group databases

TCDBi3.A.3.8.1. the p-type atpase (p-atpase) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable phospholipid-transporting ATPase IA (EC:3.6.3.1)
Alternative name(s):
ATPase class I type 8A member 1
Chromaffin granule ATPase II
Gene namesi
Name:ATP8A1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

  • Cytoplasmic vesiclesecretory vesiclechromaffin granule membrane By similarity; Multi-pass membrane protein By similarity
  • Cytoplasmic granule By similarity
  • Cell membrane By similarity
  • Endoplasmic reticulum By similarity
  • Golgi apparatus By similarity

  • Note: Exit from the endoplasmic reticulum requires the presence of TMEM30A, but not TMEM30B. In the presence of TMEM30A, predominantly located in cytoplasmic punctate structures and localizes to the plasma membrane (By similarity). Localizes to plasma membranes of red blood cells (By similarity).By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 6565CytoplasmicSequence analysisAdd
BLAST
Transmembranei66 – 8621HelicalSequence analysisAdd
BLAST
Topological domaini87 – 926ExtracellularSequence analysis
Transmembranei93 – 11523HelicalSequence analysisAdd
BLAST
Topological domaini116 – 297182CytoplasmicSequence analysisAdd
BLAST
Transmembranei298 – 31922HelicalSequence analysisAdd
BLAST
Topological domaini320 – 34425ExtracellularSequence analysisAdd
BLAST
Transmembranei345 – 36622HelicalSequence analysisAdd
BLAST
Topological domaini367 – 842476CytoplasmicSequence analysisAdd
BLAST
Transmembranei843 – 86321HelicalSequence analysisAdd
BLAST
Topological domaini864 – 87512ExtracellularSequence analysisAdd
BLAST
Transmembranei876 – 89520HelicalSequence analysisAdd
BLAST
Topological domaini896 – 92530CytoplasmicSequence analysisAdd
BLAST
Transmembranei926 – 94722HelicalSequence analysisAdd
BLAST
Topological domaini948 – 96114ExtracellularSequence analysisAdd
BLAST
Transmembranei962 – 98423HelicalSequence analysisAdd
BLAST
Topological domaini985 – 9906CytoplasmicSequence analysis
Transmembranei991 – 101121HelicalSequence analysisAdd
BLAST
Topological domaini1012 – 102918ExtracellularSequence analysisAdd
BLAST
Transmembranei1030 – 105526HelicalSequence analysisAdd
BLAST
Topological domaini1056 – 114994CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11491149Probable phospholipid-transporting ATPase IAPRO_0000046359Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei25 – 251PhosphoserineBy similarity
Modified residuei28 – 281PhosphothreonineBy similarity
Modified residuei29 – 291PhosphoserineBy similarity
Modified residuei1111 – 11111PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ29449.

Expressioni

Tissue specificityi

Kidney.

Interactioni

Subunit structurei

Interacts with TMEM30A, but not with TMEM30B.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ29449.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOVERGENiHBG050601.
InParanoidiQ29449.
KOiK14802.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR030342. ATP8A1.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PTHR24092:SF56. PTHR24092:SF56. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q29449-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTMRRTVSE IRSRAEGYEK TDDVSEKTSL ADQEEIRTIF INQPQLTKFC
60 70 80 90 100
NNHVSTAKYN IITFLPRFLY SQFRRAANSF FLFIALLQQI PDVSPTGRYT
110 120 130 140 150
TLVPLLFILA VAAIKEIIED IKRHKADNAV NKKQTQVLRN GAWEIVHWEK
160 170 180 190 200
VNVGDIVIIK GKEYIPADTV LLSSSEPQAM CYIETSNLDG ETNLKIRQGL
210 220 230 240 250
PATSDIKDID SLMRLSGRIE CESPNRHLYD FVGNIRLDGR STVPLGADQI
260 270 280 290 300
LLRGAQLRNT QWVHGIVVYT GHDTKLMQNS TSPPLKLSNV ERITNVQILI
310 320 330 340 350
LFCILIAMSL VCSVGSAIWN RRHSGRDWYL NLNYGGANNF GLNFLTFIIL
360 370 380 390 400
FNNLIPISLL VTLEVVKFTQ AYFINWDLDM HYEPTDTAAM ARTSNLNVEL
410 420 430 440 450
GQVKYIFSDK TGTLTCNVMQ FKKCTIAGVA YGQNSQFGDE KTFSDSSLLE
460 470 480 490 500
NLQNNHPTAP IICEFLTMMA VCHTAVPERE GDKIIYQAAS PDEGALVRAA
510 520 530 540 550
KQLNFVFTGR TPDSVIIDSL GQEERYELLN VLEFTSARKR MSVIVRTPSG
560 570 580 590 600
KLRLYCKGAD TVIYDRLAET SKYKEITLKH LEQFATEGLR TLCFAVAEIS
610 620 630 640 650
ESDFQEWRAV YHRASTSVQN RLLKLEESYE LIEKNLQLLG ATAIEDKLQD
660 670 680 690 700
QVPETIETLM KADIKIWILT GDKQETAINI GHSCKLRRKN MGMIVINEGS
710 720 730 740 750
LDGTRETLSR HCTTLGDALR KENDFALIID GKTLKYALTF GVRQYFLDLA
760 770 780 790 800
LSCKAVICCR VSPLQKSEVV EMVKKQVKVI TLAIGDGAND VSMIQTAHVG
810 820 830 840 850
VGISGNEGLQ AANSSDYSIA QFKYLKNLLM VHGAWNYNRG SKCILYCFYK
860 870 880 890 900
NIVLYIIEIW FAFVNGFSGQ ILFERWCIGL YNVMFTAMPP LTLGIFERSC
910 920 930 940 950
RKEYMLKYPE LYKTSQNALD FNTKVFWVHC LNGLFHSVIL FWFPLKALQY
960 970 980 990 1000
GTVFENGRTS DYLLLGNFVY TFVVITVCLK AGLETSYWTW FSHIAIWGSI
1010 1020 1030 1040 1050
ALWVVFFGIY SSLWPAVPMA PDMSGEAAML FSSGVFWMGL LFIPVASLLL
1060 1070 1080 1090 1100
DVVYKVIKRT AFKTLVDEVQ ELEAKSQDPG AVVLGKSLTE RAQLLKNVFK
1110 1120 1130 1140
KNHVNLYRSE SLQQNLLHGY AFSQDENGIV SQSEVIRAYD TTKQRPDEW
Length:1,149
Mass (Da):130,026
Last modified:January 1, 1999 - v2
Checksum:i0EE71C958E8C5BE1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51100 mRNA. Translation: AAD03352.1.
PIRiT18515.
RefSeqiNP_777263.1. NM_174838.2.
UniGeneiBt.64247.

Genome annotation databases

GeneIDi317692.
KEGGibta:317692.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U51100 mRNA. Translation: AAD03352.1.
PIRiT18515.
RefSeqiNP_777263.1. NM_174838.2.
UniGeneiBt.64247.

3D structure databases

ProteinModelPortaliQ29449.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

TCDBi3.A.3.8.1. the p-type atpase (p-atpase) superfamily.

Proteomic databases

PRIDEiQ29449.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi317692.
KEGGibta:317692.

Organism-specific databases

CTDi10396.

Phylogenomic databases

HOVERGENiHBG050601.
InParanoidiQ29449.
KOiK14802.

Enzyme and pathway databases

BRENDAi3.6.3.1. 908.

Family and domain databases

Gene3Di2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
3.40.50.1000. 2 hits.
InterProiIPR030342. ATP8A1.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006539. P-type_ATPase_IV.
IPR032631. P-type_ATPase_N.
IPR001757. P_typ_ATPase.
IPR032630. P_typ_ATPase_c.
[Graphical view]
PANTHERiPTHR24092. PTHR24092. 1 hit.
PTHR24092:SF56. PTHR24092:SF56. 1 hit.
PfamiPF00122. E1-E2_ATPase. 1 hit.
PF16212. PhoLip_ATPase_C. 1 hit.
PF16209. PhoLip_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01652. ATPase-Plipid. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAT8A1_BOVIN
AccessioniPrimary (citable) accession number: Q29449
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 1, 1999
Last modified: July 6, 2016
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.