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Q29444

- MANBA_BOVIN

UniProt

Q29444 - MANBA_BOVIN

Protein

Beta-mannosidase

Gene

MANBA

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 1 (01 Nov 1996)
      Previous versions | rss
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    Functioni

    Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides.

    Catalytic activityi

    Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei457 – 4571Proton donorBy similarity

    GO - Molecular functioni

    1. beta-mannosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. mannan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Enzyme and pathway databases

    UniPathwayiUPA00280.

    Protein family/group databases

    CAZyiGH2. Glycoside Hydrolase Family 2.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-mannosidase (EC:3.2.1.25)
    Alternative name(s):
    Lysosomal beta A mannosidase
    Mannanase
    Short name:
    Mannase
    Gene namesi
    Name:MANBA
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. lysosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Lysosome

    Pathology & Biotechi

    Involvement in diseasei

    Defects in MANBA cause beta-mannosidosis, a severe disorder that affects peripheral and central nervous system myelin resulting in tremor, nystagmus, ataxia and early death. The primary storage products associated with the enzyme deficiency are the trisaccharide Man-beta-1-4-GlcNAc-beta-1-4-GlcNAc and the disaccharide Man-beta-1-4-GlcNAc.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Chaini18 – 879862Beta-mannosidasePRO_0000012164Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi77 – 771N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi302 – 3021N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi607 – 6071N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi803 – 8031N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    The N-terminus is blocked.

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PRIDEiQ29444.

    Expressioni

    Tissue specificityi

    Highest expression is found in thyroid tissue. The amount of transcript is significantly higher in normal tissues than in tissues affected by the disease.

    Interactioni

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000026334.

    Structurei

    3D structure databases

    ProteinModelPortaliQ29444.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 2 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3250.
    HOGENOMiHOG000186862.
    HOVERGENiHBG052404.
    InParanoidiQ29444.
    KOiK01192.

    Family and domain databases

    Gene3Di2.60.120.260. 2 hits.
    2.60.40.320. 1 hit.
    3.20.20.80. 2 hits.
    InterProiIPR008979. Galactose-bd-like.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR028369. Mannanase.
    [Graphical view]
    PANTHERiPTHR10066:SF12. PTHR10066:SF12. 1 hit.
    PfamiPF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF49303. SSF49303. 3 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q29444-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLLRLLLLLA PCGAGFATKV VSISLRGNWK IHSGNGSLQL PATVPGCVHS    50
    ALFNKRIIKD PYYRFNNLDY RWIALDNWTY IKKFKLHSDM STWSKVNLVF 100
    EGIDTVAVVL LNSVPIGKTD NMFRRYSFDI THTVKAVNII EVRFQSPVVY 150
    ANQRSERHTA YWVPPNCPPP VQDGECHVNF IRKMQCSFGW DWGPSFPTQG 200
    IWKDVRIEAY NVCHLNYFMF TPIYDNYMKT WNLKIESSFD VVSSKLVSGE 250
    AIVAIPELNI QQTNNIELQH GERTVELFVK IDKAIIVETW WPHGHGNQTG 300
    YNMSVIFELD GGLRFEKSAK VYFRTVELVE EPIQNSPGLS FYFKINGLPI 350
    FLKGSNWIPA DSFQDRVTSA MLRLLLQSVV DANMNALRVW GGGVYEQDEF 400
    YELCDELGIM IWQDFMFACA LYPTDKDFMD SVREEVTHQV RRLKSHPSII 450
    TWSGNNENEA ALMMGWYDTK PGYLQTYIKD YVTLYVKNIR TIVLEGDQTR 500
    PFITSSPTNG AKTIAEGWLS PNPYDLNYGD VHFYDYVSDC WNWRTFPKAR 550
    FVSEYGYQSW PSFSTLEKVS SEEDWSYRSS FALHRQHLIN GNNEMLHQIE 600
    LHFKLPNSTD QLRRFKDTLY LTQVMQAQCV KTETEFYRRS RSEIVNGKGH 650
    TMGALYWQLN DIWQAPSWSS LEYGGKWKML HYFARHFFAP LLPVGFEDKD 700
    MLFIYGASHL HSDQQMMLTV RVHTWSSLEL VCSESTNPFV IKAGESVLLY 750
    TKPVPELLKG CPGCTRQSCV VSFYLSTDGE LLSPINYHFL SSLKNAKGLH 800
    KANITATISQ QGDTFVFDLK TSAVAPFVWL DVGSIPGRFS DNGFLMTEKT 850
    RTVFFYPWKP TSKSELEQSF HVTSLADTY 879
    Length:879
    Mass (Da):101,176
    Last modified:November 1, 1996 - v1
    Checksum:i2CA8351677E4ACFA
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti65 – 651F → R.
    Natural varianti709 – 7091H → D.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17432 mRNA. Translation: AAC48460.1.
    PIRiA55881.
    RefSeqiNP_776812.1. NM_174387.2.
    UniGeneiBt.111461.

    Genome annotation databases

    GeneIDi281909.
    KEGGibta:281909.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17432 mRNA. Translation: AAC48460.1 .
    PIRi A55881.
    RefSeqi NP_776812.1. NM_174387.2.
    UniGenei Bt.111461.

    3D structure databases

    ProteinModelPortali Q29444.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000026334.

    Protein family/group databases

    CAZyi GH2. Glycoside Hydrolase Family 2.

    Proteomic databases

    PRIDEi Q29444.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 281909.
    KEGGi bta:281909.

    Organism-specific databases

    CTDi 4126.

    Phylogenomic databases

    eggNOGi COG3250.
    HOGENOMi HOG000186862.
    HOVERGENi HBG052404.
    InParanoidi Q29444.
    KOi K01192.

    Enzyme and pathway databases

    UniPathwayi UPA00280 .

    Miscellaneous databases

    NextBioi 20805803.

    Family and domain databases

    Gene3Di 2.60.120.260. 2 hits.
    2.60.40.320. 1 hit.
    3.20.20.80. 2 hits.
    InterProi IPR008979. Galactose-bd-like.
    IPR013812. Glyco_hydro_2/20_Ig-like.
    IPR006102. Glyco_hydro_2_Ig-like.
    IPR006104. Glyco_hydro_2_N.
    IPR006103. Glyco_hydro_2_TIM.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    IPR028369. Mannanase.
    [Graphical view ]
    PANTHERi PTHR10066:SF12. PTHR10066:SF12. 1 hit.
    Pfami PF02836. Glyco_hydro_2_C. 1 hit.
    PF02837. Glyco_hydro_2_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49303. SSF49303. 3 hits.
    SSF49785. SSF49785. 1 hit.
    SSF51445. SSF51445. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 57-65; 220-228; 417-429; 617-624; 680-716; 852-861 AND 864-879.
      Tissue: Kidney and Thyroid.

    Entry informationi

    Entry nameiMANBA_BOVIN
    AccessioniPrimary (citable) accession number: Q29444
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1998
    Last sequence update: November 1, 1996
    Last modified: October 1, 2014
    This is version 98 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3