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Q29444

- MANBA_BOVIN

UniProt

Q29444 - MANBA_BOVIN

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Protein

Beta-mannosidase

Gene

MANBA

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides.

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei457 – 4571Proton donorBy similarity

GO - Molecular functioni

  1. beta-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. mannan catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

UniPathwayiUPA00280.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-mannosidase (EC:3.2.1.25)
Alternative name(s):
Lysosomal beta A mannosidase
Mannanase
Short name:
Mannase
Gene namesi
Name:MANBA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. lysosome Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Involvement in diseasei

Defects in MANBA cause beta-mannosidosis, a severe disorder that affects peripheral and central nervous system myelin resulting in tremor, nystagmus, ataxia and early death. The primary storage products associated with the enzyme deficiency are the trisaccharide Man-beta-1-4-GlcNAc-beta-1-4-GlcNAc and the disaccharide Man-beta-1-4-GlcNAc.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717Sequence AnalysisAdd
BLAST
Chaini18 – 879862Beta-mannosidasePRO_0000012164Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi35 – 351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi77 – 771N-linked (GlcNAc...)Sequence Analysis
Glycosylationi297 – 2971N-linked (GlcNAc...)Sequence Analysis
Glycosylationi302 – 3021N-linked (GlcNAc...)Sequence Analysis
Glycosylationi607 – 6071N-linked (GlcNAc...)Sequence Analysis
Glycosylationi803 – 8031N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ29444.

Expressioni

Tissue specificityi

Highest expression is found in thyroid tissue. The amount of transcript is significantly higher in normal tissues than in tissues affected by the disease.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026334.

Structurei

3D structure databases

ProteinModelPortaliQ29444.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 2 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000186862.
HOVERGENiHBG052404.
InParanoidiQ29444.
KOiK01192.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028369. Mannanase.
[Graphical view]
PANTHERiPTHR10066:SF12. PTHR10066:SF12. 1 hit.
PfamiPF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 3 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q29444-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLLRLLLLLA PCGAGFATKV VSISLRGNWK IHSGNGSLQL PATVPGCVHS
60 70 80 90 100
ALFNKRIIKD PYYRFNNLDY RWIALDNWTY IKKFKLHSDM STWSKVNLVF
110 120 130 140 150
EGIDTVAVVL LNSVPIGKTD NMFRRYSFDI THTVKAVNII EVRFQSPVVY
160 170 180 190 200
ANQRSERHTA YWVPPNCPPP VQDGECHVNF IRKMQCSFGW DWGPSFPTQG
210 220 230 240 250
IWKDVRIEAY NVCHLNYFMF TPIYDNYMKT WNLKIESSFD VVSSKLVSGE
260 270 280 290 300
AIVAIPELNI QQTNNIELQH GERTVELFVK IDKAIIVETW WPHGHGNQTG
310 320 330 340 350
YNMSVIFELD GGLRFEKSAK VYFRTVELVE EPIQNSPGLS FYFKINGLPI
360 370 380 390 400
FLKGSNWIPA DSFQDRVTSA MLRLLLQSVV DANMNALRVW GGGVYEQDEF
410 420 430 440 450
YELCDELGIM IWQDFMFACA LYPTDKDFMD SVREEVTHQV RRLKSHPSII
460 470 480 490 500
TWSGNNENEA ALMMGWYDTK PGYLQTYIKD YVTLYVKNIR TIVLEGDQTR
510 520 530 540 550
PFITSSPTNG AKTIAEGWLS PNPYDLNYGD VHFYDYVSDC WNWRTFPKAR
560 570 580 590 600
FVSEYGYQSW PSFSTLEKVS SEEDWSYRSS FALHRQHLIN GNNEMLHQIE
610 620 630 640 650
LHFKLPNSTD QLRRFKDTLY LTQVMQAQCV KTETEFYRRS RSEIVNGKGH
660 670 680 690 700
TMGALYWQLN DIWQAPSWSS LEYGGKWKML HYFARHFFAP LLPVGFEDKD
710 720 730 740 750
MLFIYGASHL HSDQQMMLTV RVHTWSSLEL VCSESTNPFV IKAGESVLLY
760 770 780 790 800
TKPVPELLKG CPGCTRQSCV VSFYLSTDGE LLSPINYHFL SSLKNAKGLH
810 820 830 840 850
KANITATISQ QGDTFVFDLK TSAVAPFVWL DVGSIPGRFS DNGFLMTEKT
860 870
RTVFFYPWKP TSKSELEQSF HVTSLADTY
Length:879
Mass (Da):101,176
Last modified:November 1, 1996 - v1
Checksum:i2CA8351677E4ACFA
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti65 – 651F → R.
Natural varianti709 – 7091H → D.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17432 mRNA. Translation: AAC48460.1.
PIRiA55881.
RefSeqiNP_776812.1. NM_174387.2.
UniGeneiBt.111461.

Genome annotation databases

GeneIDi281909.
KEGGibta:281909.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U17432 mRNA. Translation: AAC48460.1 .
PIRi A55881.
RefSeqi NP_776812.1. NM_174387.2.
UniGenei Bt.111461.

3D structure databases

ProteinModelPortali Q29444.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000026334.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Proteomic databases

PRIDEi Q29444.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 281909.
KEGGi bta:281909.

Organism-specific databases

CTDi 4126.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000186862.
HOVERGENi HBG052404.
InParanoidi Q29444.
KOi K01192.

Enzyme and pathway databases

UniPathwayi UPA00280 .

Miscellaneous databases

NextBioi 20805803.

Family and domain databases

Gene3Di 2.60.120.260. 2 hits.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028369. Mannanase.
[Graphical view ]
PANTHERi PTHR10066:SF12. PTHR10066:SF12. 1 hit.
Pfami PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 3 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 57-65; 220-228; 417-429; 617-624; 680-716; 852-861 AND 864-879.
    Tissue: Kidney and Thyroid.

Entry informationi

Entry nameiMANBA_BOVIN
AccessioniPrimary (citable) accession number: Q29444
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: October 29, 2014
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3