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Q29444

- MANBA_BOVIN

UniProt

Q29444 - MANBA_BOVIN

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Protein

Beta-mannosidase

Gene
MANBA
Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides.

Catalytic activityi

Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei457 – 4571Proton donor By similarity

GO - Molecular functioni

  1. beta-mannosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. mannan catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Enzyme and pathway databases

UniPathwayiUPA00280.

Protein family/group databases

CAZyiGH2. Glycoside Hydrolase Family 2.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-mannosidase (EC:3.2.1.25)
Alternative name(s):
Lysosomal beta A mannosidase
Mannanase
Short name:
Mannase
Gene namesi
Name:MANBA
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
ProteomesiUP000009136: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. lysosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Involvement in diseasei

Defects in MANBA cause beta-mannosidosis, a severe disorder that affects peripheral and central nervous system myelin resulting in tremor, nystagmus, ataxia and early death. The primary storage products associated with the enzyme deficiency are the trisaccharide Man-beta-1-4-GlcNAc-beta-1-4-GlcNAc and the disaccharide Man-beta-1-4-GlcNAc.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1717 Reviewed predictionAdd
BLAST
Chaini18 – 879862Beta-mannosidasePRO_0000012164Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi35 – 351N-linked (GlcNAc...) Reviewed prediction
Glycosylationi77 – 771N-linked (GlcNAc...) Reviewed prediction
Glycosylationi297 – 2971N-linked (GlcNAc...) Reviewed prediction
Glycosylationi302 – 3021N-linked (GlcNAc...) Reviewed prediction
Glycosylationi607 – 6071N-linked (GlcNAc...) Reviewed prediction
Glycosylationi803 – 8031N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiQ29444.

Expressioni

Tissue specificityi

Highest expression is found in thyroid tissue. The amount of transcript is significantly higher in normal tissues than in tissues affected by the disease.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026334.

Structurei

3D structure databases

ProteinModelPortaliQ29444.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3250.
HOGENOMiHOG000186862.
HOVERGENiHBG052404.
InParanoidiQ29444.
KOiK01192.

Family and domain databases

Gene3Di2.60.120.260. 2 hits.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProiIPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028369. Mannanase.
[Graphical view]
PANTHERiPTHR10066:SF12. PTHR10066:SF12. 1 hit.
PfamiPF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMiSSF49303. SSF49303. 3 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q29444-1 [UniParc]FASTAAdd to Basket

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MLLRLLLLLA PCGAGFATKV VSISLRGNWK IHSGNGSLQL PATVPGCVHS    50
ALFNKRIIKD PYYRFNNLDY RWIALDNWTY IKKFKLHSDM STWSKVNLVF 100
EGIDTVAVVL LNSVPIGKTD NMFRRYSFDI THTVKAVNII EVRFQSPVVY 150
ANQRSERHTA YWVPPNCPPP VQDGECHVNF IRKMQCSFGW DWGPSFPTQG 200
IWKDVRIEAY NVCHLNYFMF TPIYDNYMKT WNLKIESSFD VVSSKLVSGE 250
AIVAIPELNI QQTNNIELQH GERTVELFVK IDKAIIVETW WPHGHGNQTG 300
YNMSVIFELD GGLRFEKSAK VYFRTVELVE EPIQNSPGLS FYFKINGLPI 350
FLKGSNWIPA DSFQDRVTSA MLRLLLQSVV DANMNALRVW GGGVYEQDEF 400
YELCDELGIM IWQDFMFACA LYPTDKDFMD SVREEVTHQV RRLKSHPSII 450
TWSGNNENEA ALMMGWYDTK PGYLQTYIKD YVTLYVKNIR TIVLEGDQTR 500
PFITSSPTNG AKTIAEGWLS PNPYDLNYGD VHFYDYVSDC WNWRTFPKAR 550
FVSEYGYQSW PSFSTLEKVS SEEDWSYRSS FALHRQHLIN GNNEMLHQIE 600
LHFKLPNSTD QLRRFKDTLY LTQVMQAQCV KTETEFYRRS RSEIVNGKGH 650
TMGALYWQLN DIWQAPSWSS LEYGGKWKML HYFARHFFAP LLPVGFEDKD 700
MLFIYGASHL HSDQQMMLTV RVHTWSSLEL VCSESTNPFV IKAGESVLLY 750
TKPVPELLKG CPGCTRQSCV VSFYLSTDGE LLSPINYHFL SSLKNAKGLH 800
KANITATISQ QGDTFVFDLK TSAVAPFVWL DVGSIPGRFS DNGFLMTEKT 850
RTVFFYPWKP TSKSELEQSF HVTSLADTY 879
Length:879
Mass (Da):101,176
Last modified:November 1, 1996 - v1
Checksum:i2CA8351677E4ACFA
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti65 – 651F → R.
Natural varianti709 – 7091H → D.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U17432 mRNA. Translation: AAC48460.1.
PIRiA55881.
RefSeqiNP_776812.1. NM_174387.2.
UniGeneiBt.111461.

Genome annotation databases

GeneIDi281909.
KEGGibta:281909.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U17432 mRNA. Translation: AAC48460.1 .
PIRi A55881.
RefSeqi NP_776812.1. NM_174387.2.
UniGenei Bt.111461.

3D structure databases

ProteinModelPortali Q29444.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9913.ENSBTAP00000026334.

Protein family/group databases

CAZyi GH2. Glycoside Hydrolase Family 2.

Proteomic databases

PRIDEi Q29444.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 281909.
KEGGi bta:281909.

Organism-specific databases

CTDi 4126.

Phylogenomic databases

eggNOGi COG3250.
HOGENOMi HOG000186862.
HOVERGENi HBG052404.
InParanoidi Q29444.
KOi K01192.

Enzyme and pathway databases

UniPathwayi UPA00280 .

Miscellaneous databases

NextBioi 20805803.

Family and domain databases

Gene3Di 2.60.120.260. 2 hits.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProi IPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028369. Mannanase.
[Graphical view ]
PANTHERi PTHR10066:SF12. PTHR10066:SF12. 1 hit.
Pfami PF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view ]
SUPFAMi SSF49303. SSF49303. 3 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 57-65; 220-228; 417-429; 617-624; 680-716; 852-861 AND 864-879.
    Tissue: Kidney and Thyroid.

Entry informationi

Entry nameiMANBA_BOVIN
AccessioniPrimary (citable) accession number: Q29444
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: January 22, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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