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Q29444 (MANBA_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-mannosidase

EC=3.2.1.25
Alternative name(s):
Lysosomal beta A mannosidase
Mannanase
Short name=Mannase
Gene names
Name:MANBA
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length879 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Exoglycosidase that cleaves the single beta-linked mannose residue from the non-reducing end of all N-linked glycoprotein oligosaccharides.

Catalytic activity

Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides.

Pathway

Glycan metabolism; N-glycan degradation.

Subcellular location

Lysosome.

Tissue specificity

Highest expression is found in thyroid tissue. The amount of transcript is significantly higher in normal tissues than in tissues affected by the disease.

Post-translational modification

The N-terminus is blocked.

Involvement in disease

Defects in MANBA cause beta-mannosidosis, a severe disorder that affects peripheral and central nervous system myelin resulting in tremor, nystagmus, ataxia and early death. The primary storage products associated with the enzyme deficiency are the trisaccharide Man-beta-1-4-GlcNAc-beta-1-4-GlcNAc and the disaccharide Man-beta-1-4-GlcNAc.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Ontologies

Keywords
   Cellular componentLysosome
   Coding sequence diversityPolymorphism
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processmannan catabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentlysosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionbeta-mannosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Potential
Chain18 – 879862Beta-mannosidase
PRO_0000012164

Sites

Active site4571Proton donor By similarity

Amino acid modifications

Glycosylation351N-linked (GlcNAc...) Potential
Glycosylation771N-linked (GlcNAc...) Potential
Glycosylation2971N-linked (GlcNAc...) Potential
Glycosylation3021N-linked (GlcNAc...) Potential
Glycosylation6071N-linked (GlcNAc...) Potential
Glycosylation8031N-linked (GlcNAc...) Potential

Natural variations

Natural variant651F → R.
Natural variant7091H → D.

Sequences

Sequence LengthMass (Da)Tools
Q29444 [UniParc].

Last modified November 1, 1996. Version 1.
Checksum: 2CA8351677E4ACFA

FASTA879101,176
        10         20         30         40         50         60 
MLLRLLLLLA PCGAGFATKV VSISLRGNWK IHSGNGSLQL PATVPGCVHS ALFNKRIIKD 

        70         80         90        100        110        120 
PYYRFNNLDY RWIALDNWTY IKKFKLHSDM STWSKVNLVF EGIDTVAVVL LNSVPIGKTD 

       130        140        150        160        170        180 
NMFRRYSFDI THTVKAVNII EVRFQSPVVY ANQRSERHTA YWVPPNCPPP VQDGECHVNF 

       190        200        210        220        230        240 
IRKMQCSFGW DWGPSFPTQG IWKDVRIEAY NVCHLNYFMF TPIYDNYMKT WNLKIESSFD 

       250        260        270        280        290        300 
VVSSKLVSGE AIVAIPELNI QQTNNIELQH GERTVELFVK IDKAIIVETW WPHGHGNQTG 

       310        320        330        340        350        360 
YNMSVIFELD GGLRFEKSAK VYFRTVELVE EPIQNSPGLS FYFKINGLPI FLKGSNWIPA 

       370        380        390        400        410        420 
DSFQDRVTSA MLRLLLQSVV DANMNALRVW GGGVYEQDEF YELCDELGIM IWQDFMFACA 

       430        440        450        460        470        480 
LYPTDKDFMD SVREEVTHQV RRLKSHPSII TWSGNNENEA ALMMGWYDTK PGYLQTYIKD 

       490        500        510        520        530        540 
YVTLYVKNIR TIVLEGDQTR PFITSSPTNG AKTIAEGWLS PNPYDLNYGD VHFYDYVSDC 

       550        560        570        580        590        600 
WNWRTFPKAR FVSEYGYQSW PSFSTLEKVS SEEDWSYRSS FALHRQHLIN GNNEMLHQIE 

       610        620        630        640        650        660 
LHFKLPNSTD QLRRFKDTLY LTQVMQAQCV KTETEFYRRS RSEIVNGKGH TMGALYWQLN 

       670        680        690        700        710        720 
DIWQAPSWSS LEYGGKWKML HYFARHFFAP LLPVGFEDKD MLFIYGASHL HSDQQMMLTV 

       730        740        750        760        770        780 
RVHTWSSLEL VCSESTNPFV IKAGESVLLY TKPVPELLKG CPGCTRQSCV VSFYLSTDGE 

       790        800        810        820        830        840 
LLSPINYHFL SSLKNAKGLH KANITATISQ QGDTFVFDLK TSAVAPFVWL DVGSIPGRFS 

       850        860        870 
DNGFLMTEKT RTVFFYPWKP TSKSELEQSF HVTSLADTY 

« Hide

References

[1]"Molecular cloning and characterization of bovine beta-mannosidase."
Chen H., Leipprandt J.R., Traviss C.E., Sopher B.L., Jones M.Z., Cavanagh K.T., Friderici K.H.
J. Biol. Chem. 270:3841-3848(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 57-65; 220-228; 417-429; 617-624; 680-716; 852-861 AND 864-879.
Tissue: Kidney and Thyroid.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U17432 mRNA. Translation: AAC48460.1.
PIRA55881.
RefSeqNP_776812.1. NM_174387.2.
UniGeneBt.111461.

3D structure databases

ProteinModelPortalQ29444.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000026334.

Protein family/group databases

CAZyGH2. Glycoside Hydrolase Family 2.

Proteomic databases

PRIDEQ29444.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID281909.
KEGGbta:281909.

Organism-specific databases

CTD4126.

Phylogenomic databases

eggNOGCOG3250.
HOGENOMHOG000186862.
HOVERGENHBG052404.
InParanoidQ29444.
KOK01192.

Enzyme and pathway databases

UniPathwayUPA00280.

Family and domain databases

Gene3D2.60.120.260. 2 hits.
2.60.40.320. 1 hit.
3.20.20.80. 2 hits.
InterProIPR008979. Galactose-bd-like.
IPR013812. Glyco_hydro_2/20_Ig-like.
IPR006102. Glyco_hydro_2_Ig-like.
IPR006104. Glyco_hydro_2_N.
IPR006103. Glyco_hydro_2_TIM.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR028369. Mannanase.
[Graphical view]
PANTHERPTHR10066:SF12. PTHR10066:SF12. 1 hit.
PfamPF02836. Glyco_hydro_2_C. 1 hit.
PF02837. Glyco_hydro_2_N. 1 hit.
[Graphical view]
SUPFAMSSF49303. SSF49303. 3 hits.
SSF49785. SSF49785. 1 hit.
SSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Other

NextBio20805803.

Entry information

Entry nameMANBA_BOVIN
AccessionPrimary (citable) accession number: Q29444
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1998
Last sequence update: November 1, 1996
Last modified: January 22, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries