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Q29441 (CNGA3_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cyclic nucleotide-gated cation channel alpha-3
Alternative name(s):
Cone photoreceptor cGMP-gated channel subunit alpha
Cyclic nucleotide-gated channel alpha-3
Short name=CNG channel alpha-3
Short name=CNG-3
Short name=CNG3
Gene names
Name:CNGA3
Synonyms:CNCG3
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length706 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Visual signal transduction is mediated by a G-protein coupled cascade using cGMP as second messenger. This protein can be activated by cyclic GMP which leads to an opening of the cation channel and thereby causing a depolarization of cone photoreceptors. Essential for the generation of light-evoked electrical responses in the red-, green- and blue sensitive cones Induced a flickering channel gating, weakened the outward rectification in the presence of extracellular calcium, increased sensitivity for L-cis diltiazem and enhanced the cAMP efficacy of the channel when coexpressed with CNGB3 By similarity. Could be responsible for cGMP-induced calcium entry in cells other than sensory cells. Might be involved in chemotaxis of sperm.

Subunit structure

Tetramer formed of three CNGA3 and one CNGB3 modulatory subunits By similarity. Forms functional heterooligomeric channels with CNG4 in vitro.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Testis, kidney, retinal cone and heart.

Domain

The C-terminal coiled-coil domain mediates homotrimerization of CNGA subunits By similarity.

Sequence similarities

Belongs to the cyclic nucleotide-gated cation channel (TC 1.A.1.5) family. CNGA3 subfamily. [View classification]

Contains 1 cyclic nucleotide-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 706706Cyclic nucleotide-gated cation channel alpha-3
PRO_0000219316

Regions

Transmembrane186 – 20621Helical; Potential
Transmembrane322 – 34221Helical; Potential
Transmembrane398 – 41821Helical; Potential
Nucleotide binding501 – 624124cGMP
Coiled coil645 – 68844 By similarity

Sites

Binding site5681cGMP Potential
Binding site5831cGMP Potential

Sequences

Sequence LengthMass (Da)Tools
Q29441 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: F4990DCD29B56239

FASTA70681,132
        10         20         30         40         50         60 
MAKISTQYSH PTRTHPSVRT MDRDLDCIEN GLSRTHLPCE ETSSELQEGI AMETRGLAES 

        70         80         90        100        110        120 
RQSSFTSQGP TRLSRLIISL RAWSARHLHQ EDQRPDSFLE RFRGAELQEV SSRESHVQFN 

       130        140        150        160        170        180 
VGSQEPPDRG RSAWPLARNN TNTCNNSEKD DKAKKEEKEK KEEKKENPKK EEKKKDSVVM 

       190        200        210        220        230        240 
DPSSNMYYHW LTVIAVPVFY NWCLLVCRAC FDELQSEHLM LWLVLDYSAD ILYGMDMLVR 

       250        260        270        280        290        300 
ARTGFLEQGL MVMDASRLWK HYTQTLHFKL DVLSLVPTDL AYFKLGMNYP ELRFNRLLKL 

       310        320        330        340        350        360 
ARLFEFFDRT ETRTNYPNMF RIGNLVLYIL IIIHWNACIY FAISKFIGFG TDSWVYPNVS 

       370        380        390        400        410        420 
NPEYGRLSRK YIYSLYWSTL TLTTIGETPP PVKDEEYLFV VIDFLVGVLI FATIVGNVGS 

       430        440        450        460        470        480 
MISNMNASRA EFQAKIDSIK QYMQFRKVTK DLETRVIRWF DYLWANKKTV DEKEVLKSLP 

       490        500        510        520        530        540 
DKLKAEIAIN VHLDTLRKVR IFQDCEAGLL VELVLKLRPA VFSPGDYICK KGDIGREMYI 

       550        560        570        580        590        600 
IKEGKLAVVA EDGITQFVVL GDGSYFGEIS ILNIKGSKSG NRRTANIRSI GYSDLFCLSK 

       610        620        630        640        650        660 
DDLMEALTEY PEAKKALEEK GRQILMKDNL IDEELAKAGA DPKDIEEKVE HLETSLDSLQ 

       670        680        690        700 
TRFARLLAEY NATQMKVKQR LSQLESQVKM GLPPDGDAPQ TEASQP 

« Hide

References

[1]"Cloning and functional expression of a cyclic-nucleotide-gated channel from mammalian sperm."
Weyand I., Godde M., Frings S., Weiner J., Mueller F., Altenhofen W., Hatt H., Kaupp U.B.
Nature 368:859-863(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Testis.
[2]"Another member of the cyclic nucleotide-gated channel family, expressed in testis, kidney, and heart."
Biel M., Zong X., Distler M., Bosse E., Klugbauer N., Murakami M., Flockerzi V., Hofmann F.
Proc. Natl. Acad. Sci. U.S.A. 91:3505-3509(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Kidney.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X89600 mRNA. Translation: CAA61759.1.
X76485 mRNA. Translation: CAA54023.1.
PIRA55251.
RefSeqNP_776704.1. NM_174279.2.
XP_005212339.1. XM_005212282.1.
XP_005212340.1. XM_005212283.1.
XP_005212341.1. XM_005212284.1.
XP_005212342.1. XM_005212285.1.
XP_005212343.1. XM_005212286.1.
XP_005212344.1. XM_005212287.1.
UniGeneBt.109785.

3D structure databases

ProteinModelPortalQ29441.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000012968.

Proteomic databases

PRIDEQ29441.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000056449; ENSBTAP00000052464; ENSBTAG00000009834.
GeneID281701.
KEGGbta:281701.

Organism-specific databases

CTD1261.

Phylogenomic databases

eggNOGNOG300025.
GeneTreeENSGT00550000074376.
HOGENOMHOG000007898.
HOVERGENHBG000281.
InParanoidQ29441.
KOK04950.
OMAMAKINTQ.
OrthoDBEOG771268.
TreeFamTF319048.

Family and domain databases

Gene3D2.60.120.10. 1 hit.
InterProIPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR005821. Ion_trans_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamPF00027. cNMP_binding. 1 hit.
PF00520. Ion_trans. 1 hit.
[Graphical view]
SMARTSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
PROSITEPS00888. CNMP_BINDING_1. 1 hit.
PS00889. CNMP_BINDING_2. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805624.

Entry information

Entry nameCNGA3_BOVIN
AccessionPrimary (citable) accession number: Q29441
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families