Q29408 (IL10_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interleukin-10
Short name=IL-10

Alternative name(s):
Cytokine synthesis inhibitory factor
Short name=CSIF

Gene names

Name:

IL10

Organism

Ovis aries (Sheep)

Taxonomic identifier

9940 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Caprinae › Ovis

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the IL-10 family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological_process	immune response Inferred from electronic annotation. Source: InterPro inflammatory response Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of B cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cytokine secretion involved in immune response Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of interleukin-6 production Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of membrane protein ectodomain proteolysis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of B cell apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cytokine secretion Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of sequence-specific DNA binding transcription factor activity Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB receptor biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB response to glucocorticoid stimulus Inferred from sequence or structural similarity. Source: UniProtKB response to molecule of bacterial origin Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	extracellular space Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	interleukin-10 receptor binding Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 177	158	Interleukin-10		PRO_0000015373
Amino acid modifications
	Glycosylation	135	1	N-linked (GlcNAc...) Potential
	Disulfide bond	31 ↔ 127		By similarity
	Disulfide bond	81 ↔ 133		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q29408 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: EF60025499E29BED	FASTA	177	20,189
	10 20 30 40 50 60 MPSSSAVLCC LVFLAGVAAS RDASTLSDSS CTHFPASLPH MLRDVRAAFG KVKTFFQMKD 70 80 90 100 110 120 QLNSMLLTQS LLDDFKGYLG CQALSEMIQF YLEEVMPQAE NHGPDIKEHV NSLGEKLKTL 130 140 150 160 170 RLRLRRCHRF LPCENKSKAV EQVKRVFNML QERGVYKAMS EFDIFINYIE SYMTTKM

References

[1]	"Cloning and characterisation of an ovine interleukin-10-encoding cDNA." Martin H.M., Nash A.D., Andrews A.E. Gene 159:187-191(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Macrophage.
[2]	"Sequence of the sheep interleukin-10-encoding cDNA." Dutia B.M., Hunt P., Sargan D.R., Dalziel R.G., Hopkins J. Gene 149:393-394(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Macrophage.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U11421 mRNA. Translation: AAC13768.1. Z29362 mRNA. Translation: CAA82546.1.
RefSeq	NP_001009327.1. NM_001009327.1.
UniGene	Oar.528.
3D structure databases
ProteinModelPortal	Q29408.
SMR	Q29408. Positions 25-177.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	443342.
Organism-specific databases
CTD	3586.
Phylogenomic databases
HOVERGEN	HBG004110.
Family and domain databases
Gene3D	1.20.1250.10. 1 hit.
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012351. 4_helix_cytokine_core. IPR020443. Interleukin-10/19/20/24. IPR020423. Interleukin-10_CS. IPR000098. Interleukin_10. [Graphical view]
PANTHER	PTHR11585. PTHR11585. 1 hit.
Pfam	PF00726. IL10. 1 hit. [Graphical view]
PRINTS	PR01294. INTRLEUKIN10.
SMART	SM00188. IL10. 1 hit. [Graphical view]
SUPFAM	SSF47266. 4_helix_cytokine. 1 hit.
PROSITE	PS00520. INTERLEUKIN_10. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IL10_SHEEP

Accession

Primary (citable) accession number: Q29408

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program