Q29406 (LEP_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Leptin

Alternative name(s):
Obesity factor

Gene names

Name:	LEP
Synonyms:	OB, OBS

Organism

Sus scrofa (Pig) [Reference proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	167 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
Subcellular location	Secreted Probable.
Sequence similarities	Belongs to the leptin family.

Ontologies

Keywords
Cellular component	Secreted
Disease	Obesity
Domain	Signal
PTM	Disulfide bond
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adipose tissue development Inferred from electronic annotation. Source: Compara adult feeding behavior Inferred from electronic annotation. Source: Compara bile acid metabolic process Inferred from electronic annotation. Source: Compara central nervous system neuron development Inferred from electronic annotation. Source: Compara cholesterol metabolic process Inferred from electronic annotation. Source: Compara eating behavior Inferred from electronic annotation. Source: Compara glucose metabolic process Inferred from electronic annotation. Source: Compara hormone metabolic process Inferred from electronic annotation. Source: Compara insulin secretion Inferred from electronic annotation. Source: Compara negative regulation of appetite Inferred from electronic annotation. Source: Compara negative regulation of glucagon secretion Inferred from electronic annotation. Source: Compara negative regulation of transcription from RNA polymerase II promoter Inferred from electronic annotation. Source: Compara placenta development Inferred from electronic annotation. Source: Compara positive regulation of STAT protein import into nucleus Inferred from electronic annotation. Source: Compara positive regulation of developmental growth Inferred from electronic annotation. Source: Compara positive regulation of myeloid cell differentiation Inferred from electronic annotation. Source: Compara regulation of gluconeogenesis Inferred from electronic annotation. Source: Compara regulation of insulin secretion Inferred from electronic annotation. Source: Compara regulation of intestinal cholesterol absorption Inferred from electronic annotation. Source: Compara regulation of protein phosphorylation Inferred from electronic annotation. Source: Compara regulation of steroid biosynthetic process Inferred from electronic annotation. Source: Compara response to dietary excess Inferred from electronic annotation. Source: Compara response to insulin stimulus Inferred from electronic annotation. Source: Compara tyrosine phosphorylation of STAT protein Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular space Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 167	146	Leptin		PRO_0000017689
Amino acid modifications
	Disulfide bond	117 ↔ 167		By similarity
Experimental info
	Sequence conflict	21 – 22	2	AV → GP in AAC48641. Ref.8
	Sequence conflict	97	1	I → L in AAC48641. Ref.8
	Sequence conflict	122	1	A → R in AAB97308. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Q29406 [UniParc]. Last modified November 1, 1997. Version 1. Checksum: 27550E1E0E63814E	FASTA	167	18,661
	10 20 30 40 50 60 MRCGPLCRFL WLWPYLSYVE AVPIWRVQDD TKTLIKTIVT RISDISHMQS VSSKQRVTGL 70 80 90 100 110 120 DFIPGLHPVL SLSKMDQTLA IYQQILTSLP SRNVIQISND LENLRDLLHL LASSKSCPLP 130 140 150 160 QARALETLES LGGVLEASLY STEVVALSRL QGALQDMLRQ LDLSPGC

References

[1]	"Cloning and tissue distribution of leptin in the pig." Louis C.F. Submitted (AUG-1996) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adipose tissue.
[2]	Robert C., Palin M.-F., Coulombe N., Roberge C., Silversides F.G., Benkel B.F., McKay R.M., Pelletier G. Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Landrace.
[3]	"Cloning and expression of the porcine obese gene." Bidwell C.A., Ji S., Frank G.R., Cornelius S.G., Willis G.M., Spurlock M.E. Anim. Biotechnol. 8:191-206(1997) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The obesity gene in swine: sequence and expression of porcine leptin." Ramsay T.G., Yan X., Morrison C. J. Anim. Sci. 76:484-490(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[5]	"Molecular cloning and analysing of porcine obese cDNA." Dai R.J., Li N., Wu C.X. Yi Chuan Xue Bao 27:290-297(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Meishan.
[6]	"Adipose tissue regulatory transcript expression in lean versus obese pigs." McNeel R.L., Mersmann H.J. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[7]	Soares M.A.M., Euclydes R.F., Guimaraes S.E.F., Martins M.F., Lopes P.S. Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Large white.
[8]	"Partial characterization of porcine obesity gene (OBS) and its localization to chromosome 18 by somatic cell hybrids." Neuenschwander S., Rettenberger G., Meijerink E., Jorg H., Stranzinger G. Anim. Genet. 27:275-278(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 15-110. Tissue: White adipose tissue.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U63540 mRNA. Translation: AAB05923.1. AF026976 mRNA. Translation: AAB82724.1. U66254 Genomic DNA. Translation: AAB97308.1. U59894 mRNA. Translation: AAB03458.1. AF052691 mRNA. Translation: AAC06303.1. AF102856 mRNA. Translation: AAC78147.1. AF477387, AF477386 Genomic DNA. Translation: AAL84792.1. U40812 mRNA. Translation: AAC48641.1.
RefSeq	NP_999005.1. NM_213840.1.
UniGene	Ssc.265.
3D structure databases
ProteinModelPortal	Q29406.
ModBase	Search...
Protein-protein interaction databases
STRING	9823.ENSSSCP00000017572.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSSSCT00000018060; ENSSSCP00000017572; ENSSSCG00000016588.
GeneID	396832.
KEGG	ssc:396832.
Organism-specific databases
CTD	3952.
Phylogenomic databases
eggNOG	NOG45133.
GeneTree	ENSGT00390000011772.
HOGENOM	HOG000252923.
HOVERGEN	HBG007860.
KO	K05424.
OMA	KTIVTRI.
OrthoDB	EOG4QNMXC.
Gene expression databases
ArrayExpress	Q29406.
Family and domain databases
Gene3D	1.20.1250.10. 1 hit.
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012351. 4_helix_cytokine_core. IPR000065. Leptin. [Graphical view]
PANTHER	PTHR11724. PTHR11724. 1 hit.
Pfam	PF02024. Leptin. 1 hit. [Graphical view]
PIRSF	PIRSF001837. Leptin. 1 hit.
PRINTS	PR00495. LEPTIN.
ProDom	PD005698. Leptin. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF47266. 4_helix_cytokine. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEP_PIG

Accession

Primary (citable) accession number: Q29406
Secondary accession number(s): O19095, Q95251

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	November 1, 1997
Last modified:	April 3, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program