Q29381 (OST48_PIG) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit
Short name=DDOST 48 kDa subunit
Short name=Oligosaccharyl transferase 48 kDa subunit
EC=2.4.1.119

Gene names

Name:	DDOST
Synonyms:	OST48

Organism

Sus scrofa (Pig) [Complete proteome]

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	439 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. Ref.1
Catalytic activity	Dolichyl diphosphooligosaccharide + protein L-asparagine = dolichyl diphosphate + a glycoprotein with the oligosaccharide chain attached by N-glycosyl linkage to protein L-asparagine.
Pathway	Protein modification; protein glycosylation.
Subunit structure	Component of the oligosaccharyltransferase (OST) complex. OST seems to exist in different forms which contain at least RPN1, RPN2, OST48, DAD1, OSTC, KRTCAP2 and either STT3A or STT3B. OST can form stable complexes with the Sec61 complex or with both the Sec61 and TRAP complexes even after release from the ribosome By similarity.
Subcellular location	Endoplasmic reticulum membrane; Single-pass type I membrane protein Ref.1.
Sequence similarities	Belongs to the DDOST 48 kDa subunit family.

Ontologies

Keywords
Cellular component	Endoplasmic reticulum Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	protein N-linked glycosylation via asparagine Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW oligosaccharyltransferase complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	dolichyl-diphosphooligosaccharide-protein glycotransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Ref.1
	Chain	26 – 439	414	Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 48 kDa subunit		PRO_0000058095
Regions
	Topological domain	27 – 410	384	Lumenal Potential
	Transmembrane	411 – 430	20	Helical; Potential
	Topological domain	431 – 439	9	Cytoplasmic Potential
Experimental info
	Mutagenesis	437	1	K → L: Abolishes ER localization resulting in plasma membrane localization. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q29381 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: 480F8196B14F8C72	FASTA	439	48,863
	10 20 30 40 50 60 MELGAAARAW SLLWLLLPLL GLVGASGPRT LVLLDNLNLR ETHSLFFRSL KDRGFVLTFK 70 80 90 100 110 120 TADDPSLSLI KYGEFLYDNL IVFSPSVEDF GGNINVETIS TFIDGGGSVL VAASSDIGDP 130 140 150 160 170 180 LRELGSECGI EFDEEKTAVI DHHNYDVSDL AQHTLIVADT ENLLKAPTIV GKSSLNPILF 190 200 210 220 230 240 RGVGMVADPD NPLVLDILTG SSTSYSFFPD KPITQYPHAV GKNTLLIAGL QARNNARVIF 250 260 270 280 290 300 SGSLDFFSDA FFNSAVQKAT PGSQRYPQTG NYELAVALSR WVFKEEGVLR VGPVSHHRVG 310 320 330 340 350 360 EKAPPNAYTV TDLVEYSIVI EQLSEGRWVP FDGDDIQLEF VRIDPFVRTF LKRKGGKYSV 370 380 390 400 410 420 QFKFPDVYGV FQFKVDYNRL GYTHLYSSTQ VSVRPLQHTQ YERFIPSAYP YYASAFSMMV 430 GLFIFSVVFL HMKEKEKSD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The oligosaccharyltransferase complex from pig liver: cDNA cloning, expression and functional characterisation." Hardt B., Aparicio R., Bause E. Glycoconj. J. 17:767-779(2000) [PubMed: 11443278] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 26-43, MUTAGENESIS OF LYS-437, FUNCTION, SUBCELLULAR LOCATION. Tissue: Liver.
[2]	"Evaluation and characterization of a porcine small intestine cDNA library: analysis of 839 clones." Winteroe A.K., Fredholm M., Davies W. Mamm. Genome 7:509-517(1996) [PubMed: 8672129] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 27-129. Tissue: Small intestine.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ293581 mRNA. Translation: CAC10570.1. F14593 mRNA. Translation: CAA23144.1.
RefSeq	NP_999353.1. NM_214188.1.
UniGene	Ssc.5550.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q29381.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	397385.
KEGG	ssc:397385.
Organism-specific databases
CTD	31849.
Phylogenomic databases
GeneTree	ENSGT00390000017294.
HOVERGEN	HBG053378.
OrthoDB	EOG45HRXC.
Family and domain databases
InterPro	IPR005013. OligosaccharylTrfase_su_Wbp1. [Graphical view]
KO	K12670.
PANTHER	PTHR10830. DDOST_48kDa. 1 hit.
Pfam	PF03345. DDOST_48kD. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OST48_PIG

Accession

Primary (citable) accession number: Q29381
Secondary accession number(s): Q9GL02

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1998
Last sequence update:	December 16, 2008
Last modified:	December 14, 2011
This is version 63 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program