ID   SYCC_DROPS              Reviewed;         741 AA.
AC   Q291L4;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   04-APR-2006, sequence version 1.
DT   16-JUN-2009, entry version 29.
DE   RecName: Full=Cysteinyl-tRNA synthetase, cytoplasmic;
DE            EC=6.1.1.16;
DE   AltName: Full=Cysteine--tRNA ligase;
DE            Short=CysRS;
GN   Name=Aats-cys; ORFNames=GA21073;
OS   Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC   Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha;
OC   Ephydroidea; Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=46245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MV2-25;
RX   PubMed=15632085; DOI=10.1101/gr.3059305;
RA   Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA   Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P.,
RA   Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J.,
RA   van Batenburg M.F., Howells S.L., Scherer S.E., Sodergren E.,
RA   Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D.,
RA   Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D.,
RA   Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R.,
RA   Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L.,
RA   Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA   Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA   Weinstock G.M., Gibbs R.A.;
RT   "Comparative genome sequencing of Drosophila pseudoobscura:
RT   chromosomal, gene, and cis-element evolution.";
RL   Genome Res. 15:1-18(2005).
CC   -!- CATALYTIC ACTIVITY: ATP + L-cysteine + tRNA(Cys) = AMP +
CC       diphosphate + L-cysteinyl-tRNA(Cys).
CC   -!- COFACTOR: Binds 1 zinc ion per subunit (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase
CC       family.
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DR   EMBL; CM000071; EAL25098.1; -; Genomic_DNA.
DR   RefSeq; XP_001360523.1; -.
DR   GeneID; 4803874; -.
DR   KEGG; dpo:Dpse_GA21073; -.
DR   FlyBase; FBgn0081061; Dpse\GA21073.
DR   OMA; Q291L4; QCNLYMA.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004817; F:cysteine-tRNA ligase activity; ISS:UniProtKB.
DR   GO; GO:0000049; F:tRNA binding; ISS:UniProtKB.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006423; P:cysteinyl-tRNA aminoacylation; ISS:UniProtKB.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR015804; Cys-tRNA-synt_Ia_C.
DR   InterPro; IPR015803; Cys-tRNA-synt_Ia_N.
DR   InterPro; IPR002308; Cys-tRNA-synth_1a.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   Gene3D; G3DSA:3.40.50.620; Rossmann-like_a/b/a_fold; 1.
DR   PANTHER; PTHR10890; Cys_tRNA-synt_1a; 1.
DR   Pfam; PF01406; tRNA-synt_1e; 1.
DR   PRINTS; PR00983; TRNASYNTHCYS.
DR   TIGRFAMs; TIGR00435; cysS; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; FALSE_NEG.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Complete proteome; Cytoplasm;
KW   Ligase; Metal-binding; Nucleotide-binding; Phosphoprotein;
KW   Protein biosynthesis; Zinc.
FT   CHAIN         1    741       Cysteinyl-tRNA synthetase, cytoplasmic.
FT                                /FTId=PRO_0000348219.
FT   MOTIF        48     58       "HIGH" region (By similarity).
FT   MOTIF       398    402       "KMSKS" region (By similarity).
FT   METAL        46     46       Zinc (By similarity).
FT   METAL       340    340       Zinc (By similarity).
FT   METAL       365    365       Zinc (By similarity).
FT   METAL       369    369       Zinc (By similarity).
FT   BINDING     401    401       ATP (By similarity).
FT   MOD_RES     297    297       Phosphoserine (By similarity).
SQ   SEQUENCE   741 AA;  83780 MW;  83B894351E0A8165 CRC64;
     MSKRVQPAWQ APKPAERPKL RLYNSLTRQK EDFVPLDGNN VTWYSCGPTV YDASHMGHAR
     SYISFDILRR ILADYFGYNI HYVMNITDID DKIIKRARQN HLFEEYAGES ANLPLDQLLG
     HQKEVLVRFQ ETCAKNTDPD KKVMLDKTLQ RMNDAVVALT TAVSQGDEKG IAEKRQHYLN
     EAKDPIAEWL DGKKGAEIND NAVFESLPRF WEDQFHNDMK SLNILPPDVL TRVSEYVPQI
     VAFIQKIIDN GLAYAANNSV YFDVNGFDRK EKHHYAKLVP EAYGDTKSLQ EGEGDLSVAE
     DRLSEKRSAN DFALWKASKA GEPWWDSPWG RGRPGWHIEC SAMASDIFGS TFDIHTGGVD
     LKFPHHDNEL AQSEAAFNES EWVKYFLHTG HLTIAGCKMS KSLKNFVTIQ EALKKHSATQ
     LRLAFLLHSW KDTLDYSENT MEMATQYEKF LNEFFLNVKD LTRHVLSEEP RRQFDAWTDV
     EAALQKKFSS SQVQVHAALC DNVDTRSALD AIRELVSASN VYIRDNKSRL NSLLLRNVAT
     YITDLLHVFG AIAGPRGGIG FPVSGGAGPQ AAGGDLETTV LPYVQTLAEF RNLVREQAKA
     LKAFDILKLC DDLRDNILPN LGVRLEDKDG GKFAVKLVDR DSLLREREAK LAAEAEKAAE
     KERKKQAVAA AAAAKDAQRR VNPKQMFLGE TEKYSAFDEN GLPTLDKEGK EISKGQVKKL
     QKLQQQQEQR YKEYLASIKE A
//