Reviewed, UniProtKB/Swiss-Prot Q291L4 (SYCC_DROPS)

Names and origin

Protein names

Recommended name:
    Cysteinyl-tRNA synthetase, cytoplasmic
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase
      Short name=CysRS

Gene names

Name:	Aats-cys
ORF Names:	GA21073

Organism

Drosophila pseudoobscura pseudoobscura (Fruit fly) [Complete proteome]

Taxonomic identifier

46245 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	741 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys).
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from sequence or structural similarity. Source: UniProtKB tRNA binding Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 741	741	Cysteinyl-tRNA synthetase, cytoplasmic		PRO_0000348219
Regions
	Motif	48 – 58	11	"HIGH" region By similarity
	Motif	398 – 402	5	"KMSKS" region By similarity
Sites
	Metal binding	46	1	Zinc By similarity
	Metal binding	340	1	Zinc By similarity
	Metal binding	365	1	Zinc By similarity
	Metal binding	369	1	Zinc By similarity
	Binding site	401	1	ATP By similarity
Amino acid modifications
	Modified residue	297	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q291L4-1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 83B894351E0A8165	FASTA	741	83,780
	10 20 30 40 50 60 MSKRVQPAWQ APKPAERPKL RLYNSLTRQK EDFVPLDGNN VTWYSCGPTV YDASHMGHAR 70 80 90 100 110 120 SYISFDILRR ILADYFGYNI HYVMNITDID DKIIKRARQN HLFEEYAGES ANLPLDQLLG 130 140 150 160 170 180 HQKEVLVRFQ ETCAKNTDPD KKVMLDKTLQ RMNDAVVALT TAVSQGDEKG IAEKRQHYLN 190 200 210 220 230 240 EAKDPIAEWL DGKKGAEIND NAVFESLPRF WEDQFHNDMK SLNILPPDVL TRVSEYVPQI 250 260 270 280 290 300 VAFIQKIIDN GLAYAANNSV YFDVNGFDRK EKHHYAKLVP EAYGDTKSLQ EGEGDLSVAE 310 320 330 340 350 360 DRLSEKRSAN DFALWKASKA GEPWWDSPWG RGRPGWHIEC SAMASDIFGS TFDIHTGGVD 370 380 390 400 410 420 LKFPHHDNEL AQSEAAFNES EWVKYFLHTG HLTIAGCKMS KSLKNFVTIQ EALKKHSATQ 430 440 450 460 470 480 LRLAFLLHSW KDTLDYSENT MEMATQYEKF LNEFFLNVKD LTRHVLSEEP RRQFDAWTDV 490 500 510 520 530 540 EAALQKKFSS SQVQVHAALC DNVDTRSALD AIRELVSASN VYIRDNKSRL NSLLLRNVAT 550 560 570 580 590 600 YITDLLHVFG AIAGPRGGIG FPVSGGAGPQ AAGGDLETTV LPYVQTLAEF RNLVREQAKA 610 620 630 640 650 660 LKAFDILKLC DDLRDNILPN LGVRLEDKDG GKFAVKLVDR DSLLREREAK LAAEAEKAAE 670 680 690 700 710 720 KERKKQAVAA AAAAKDAQRR VNPKQMFLGE TEKYSAFDEN GLPTLDKEGK EISKGQVKKL 730 740 QKLQQQQEQR YKEYLASIKE A

References

[1]

"Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution." Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L. , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A. Genome Res. 15:1-18(2005) [PubMed: 15632085] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MV2-25.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CM000071 Genomic DNA. Translation: EAL25098.1.
RefSeq	XP_001360523.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4803874.
KEGG	dpo:Dpse_GA21073.
Organism-specific databases
FlyBase	FBgn0081061. Dpse\GA21073.
Phylogenomic databases
OMA	Q291L4. QCNLYMA.
Family and domain databases
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015803. Cys-tRNA-synt_Ia_N. IPR002308. Cys-tRNA-synth_1a. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR00435. cysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYCC_DROPS

Accession

Primary (citable) accession number: Q291L4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	April 4, 2006
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project