Q291J4 (CALYP_DROPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase calypso
EC=3.4.19.12

Alternative name(s):
BAP1 homolog

Gene names

Name:	calypso
ORF Names:	GA21084

Organism

Drosophila pseudoobscura pseudoobscura (Fruit fly) [Reference proteome]

Taxonomic identifier

46245 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › obscura group › pseudoobscura subgroup › Drosophila pseudoobscura

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Polycomb group (PcG) protein. Catalytic component of the PR-DUB complex, a complex that specifically mediates deubiquitination of histone H2A monoubiquitinated at 'Lys-118' (H2AK118ub1). Does not deubiquitinate monoubiquitinated histone H2B. Required to maintain the transcriptionally repressive state of homeotic genes throughout development. The PR-DUB complex has weak or no activity toward 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Subunit structure	Component of the PR-DUB complex, at least composed of calypso and Asx By similarity.
Subcellular location	Nucleus By similarity. Note: Localizes to PcG response elements (PREs) By similarity.
Sequence similarities	Belongs to the peptidase C12 family. BAP1 subfamily.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Molecular function	Chromatin regulator Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	chromatin silencing Inferred from sequence or structural similarity. Source: UniProtKB monoubiquitinated histone H2A deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	PR-DUB complex Inferred from sequence or structural similarity. Source: UniProtKB nuclear chromatin Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	chromatin binding Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 475	475	Ubiquitin carboxyl-terminal hydrolase calypso		PRO_0000395831
Sites
	Active site	130	1	Nucleophile By similarity
	Active site	212	1	Proton donor By similarity
	Site	227	1	Important for enzyme activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q291J4 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 02746ECD4125FDD8	FASTA	475	52,100
	10 20 30 40 50 60 MNVAAGGPGT ASASTTAANS IFNNSLLASA TGATTMPMAQ LADGWLELES DPGLFTLLLE 70 80 90 100 110 120 DFGCHDVQVE EVYDLQKPIE SPYGFIFLFR WIEERRARRK IVETTAEIFV KDEEAISSIF 130 140 150 160 170 180 FAQQVVPNSC ATHALLSVLL NCNENNLQLG DTLSRLKVHT KGMSPENKGL AIGNTPELAC 190 200 210 220 230 240 AHNSHAIPQA RRRLERTGAG VASCRFTGEA FHFVSFVPIS GQLFELDGLK PYPMNHGGWE 250 260 270 280 290 300 DHEDWTDKFR RVMAERLGIA TGEQDIRFNL MAVVPDRRIA ITHKLKMLRT NQAIVSGTLQ 310 320 330 340 350 360 KLLKADEQGE SGNGDQQRPD TPTTLLEPSA FTAKDLQLLL KNLDTEIAIN EQNLADENDR 370 380 390 400 410 420 RHMFKVDASR RTHNYDKFIC TFLSMLAHQG VLGELVSQHL LPSKKVSGQS AANRISKQNS 430 440 450 460 470 AASSAGANAG AAAGVTPKSQ QQQQQPQTAA SKNGKSPGKT PGRRRKGRNK CRKRK

References

[1]

"Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution." Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L. , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A. Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MV2-25 / Tucson 14011-0121.94.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CM000071 Genomic DNA. Translation: EAL25118.1.
RefSeq	XP_001360543.1. XM_001360506.2.
3D structure databases
ProteinModelPortal	Q291J4.
ModBase	Search...
Protein family/group databases
MEROPS	C12.A09.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0278406; FBpp0276844; FBgn0081072.
GeneID	4803898.
KEGG	dpo:Dpse_GA21084.
Organism-specific databases
FlyBase	FBgn0081072. Dpse\GA21084.
Phylogenomic databases
eggNOG	NOG249036.
InParanoid	Q291J4.
KO	K08588.
OMA	DKFRRVM.
OrthoDB	EOG4FN302.
Family and domain databases
Gene3D	3.40.532.10. 1 hit.
InterPro	IPR001578. Peptidase_C12. [Graphical view]
PANTHER	PTHR10589. PTHR10589. 1 hit.
Pfam	PF01088. Peptidase_C12. 1 hit. [Graphical view]
PRINTS	PR00707. UBCTHYDRLASE.
PROSITE	PS00140. UCH_1. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CALYP_DROPS

Accession

Primary (citable) accession number: Q291J4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 13, 2010
Last sequence update:	April 4, 2006
Last modified:	May 1, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project