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Q29092

- ENPL_PIG

UniProt

Q29092 - ENPL_PIG

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Protein

Endoplasmin

Gene
HSP90B1, GRP94, TRA1
Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei107 – 1071ATP By similarity
Binding sitei149 – 1491ATP By similarity
Binding sitei162 – 1621ATP By similarity
Binding sitei168 – 1681ATP By similarity
Binding sitei199 – 1991ATP; via amide nitrogen By similarity
Binding sitei448 – 4481ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
  2. protein folding Source: InterPro
  3. response to stress Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Ligandi

ATP-binding, Calcium, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Endoplasmin
Alternative name(s):
94 kDa glucose-regulated protein
Short name:
GRP-94
98 kDa protein kinase
Short name:
PPK 98
Short name:
ppk98
Heat shock protein 90 kDa beta member 1
gp96 homolog
Gene namesi
Name:HSP90B1
Synonyms:GRP94, TRA1
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. endoplasmic reticulum lumen Source: UniProtKB-SubCell
  2. melanosome Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121 Reviewed predictionAdd
BLAST
Chaini22 – 804783EndoplasminUniRule annotationPRO_0000013600Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi62 – 621N-linked (GlcNAc...) Reviewed prediction
Glycosylationi107 – 1071N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi138 – 138Interchain By similarity
Glycosylationi217 – 2171N-linked (GlcNAc...) Reviewed prediction
Modified residuei306 – 3061Phosphoserine By similarity
Modified residuei404 – 4041N6-succinyllysine By similarity
Glycosylationi445 – 4451N-linked (GlcNAc...) Reviewed prediction
Modified residuei479 – 4791N6-acetyllysine By similarity
Glycosylationi481 – 4811N-linked (GlcNAc...) Reviewed prediction
Glycosylationi502 – 5021N-linked (GlcNAc...) Reviewed prediction
Modified residuei633 – 6331N6-succinyllysine By similarity

Post-translational modificationi

Phosphorylated By similarity.UniRule annotation

Keywords - PTMi

Acetylation, Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ29092.
PRIDEiQ29092.

Interactioni

Subunit structurei

Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 By similarity. Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9 By similarity. Interacts with CNPY3; this interaction is disrupted in the presence of ATP. Interacts with several TLRs, including TLR4 and TLR9, but not with TLR3 By similarity. Interacts with MZB1 in a calcium-dependent manner By similarity. Interacts with METTL23 By similarity.

Structurei

3D structure databases

ProteinModelPortaliQ29092.
SMRiQ29092. Positions 69-755.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi801 – 8044Prevents secretion from ER Reviewed prediction

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG0326.
HOGENOMiHOG000031988.
HOVERGENiHBG007374.
KOiK09487.

Family and domain databases

Gene3Di3.30.565.10. 2 hits.
HAMAPiMF_00505. HSP90.
InterProiIPR003594. HATPase_ATP-bd.
IPR019805. Heat_shock_protein_90_CS.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view]
PANTHERiPTHR11528. PTHR11528. 1 hit.
PfamiPF00183. HSP90. 1 hit.
[Graphical view]
PIRSFiPIRSF002583. Hsp90. 1 hit.
PRINTSiPR00775. HEATSHOCK90.
SMARTiSM00387. HATPase_c. 1 hit.
[Graphical view]
SUPFAMiSSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS00014. ER_TARGET. 1 hit.
PS00298. HSP90. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q29092-1 [UniParc]FASTAAdd to Basket

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MRALWVLGLC CVLLTFGSVR AEDEVDVDGT VEEDLGKSRE GSRTDDEVVQ    50
REEEAIQLDG LNASQIRELR EKSEKFAFQA EVNRMMKLII NSLYKNKEIF 100
LRELISNASD ALDKIRLISL TDENALAGNE ELTVKIKCDK EKNLLHVTDT 150
GVGMTREELV KNLGTIAKSG TSEFLNKMAE AQEDGQSTSE LIGQFGVGFY 200
SAFLVADKVI VTSKHNNDTQ HIWESDSNEF SVIADPRGNT LGRGTTITLV 250
LKEEASDYLE LDTIKNLVKK YSQFINFPIY VWSSKTETVE EPMEEEEAAK 300
EEKEESDDEA AVEEEEEEKK PKTKKVEKTV WDWELMNDIK PIWQRPSKEV 350
EDDEYKAFYK SFSKESDDPM AYIHFTAEGE VTFKSILFVP TSAPRGLFDE 400
YGSKKSDYIK LYVRRVFITD DFHDMMPKYL NFVKGVVDSD DLPLNVSRET 450
LQQHKLLKVI RKKLVRKTLD MIKKIADEKY NDTFWKEFGT NIKLGVIEDH 500
SNRTRLAKLL RFQSSHHPSD ITSLDQYVER MKEKQDKIYF MAGSSRKEAE 550
SSPFVERLLK KGYEVIYLTE PVDEYCIQAL PEFDGKRFQN VAKEGVKFDE 600
SEKSKENREA VEKEFEPLLN WMKDKALKDK IEKAVVSQRL TESPCALVAS 650
QYGWSGNMER IMKAQAYQTG KDISTNYYAS QKKTFEINPR HPLIRDMLRR 700
VKEDEDDKTV SDLAVVLFET ATLRSGYLLP DTKAYGDRIE RMLRLSLNID 750
PDAKVEEEPE EEPEETTEDT TEDTEQDDDE EMDAGADEEE QETSETSTAE 800
KDEL 804
Length:804
Mass (Da):92,471
Last modified:December 13, 2001 - v3
Checksum:iFF823A77406F0190
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti48 – 481V → I in CAA70347. 1 Publication
Sequence conflicti179 – 1791A → T in CAA70347. 1 Publication
Sequence conflicti332 – 3343DWE → ELG in CAA62352. 1 Publication
Sequence conflicti795 – 7951E → EFVFQ in CAA62352. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76301 mRNA. Translation: CAA53948.1.
Y09136 mRNA. Translation: CAA70347.1.
X90848 Genomic DNA. Translation: CAA62352.1.
PIRiPL0137.
S51358.
RefSeqiNP_999268.1. NM_214103.1.
UniGeneiSsc.94181.
Ssc.9889.

Genome annotation databases

GeneIDi397191.
KEGGissc:397191.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76301 mRNA. Translation: CAA53948.1 .
Y09136 mRNA. Translation: CAA70347.1 .
X90848 Genomic DNA. Translation: CAA62352.1 .
PIRi PL0137.
S51358.
RefSeqi NP_999268.1. NM_214103.1.
UniGenei Ssc.94181.
Ssc.9889.

3D structure databases

ProteinModelPortali Q29092.
SMRi Q29092. Positions 69-755.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi Q29092.
PRIDEi Q29092.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 397191.
KEGGi ssc:397191.

Organism-specific databases

CTDi 7184.

Phylogenomic databases

eggNOGi COG0326.
HOGENOMi HOG000031988.
HOVERGENi HBG007374.
KOi K09487.

Miscellaneous databases

PROi Q29092.

Family and domain databases

Gene3Di 3.30.565.10. 2 hits.
HAMAPi MF_00505. HSP90.
InterProi IPR003594. HATPase_ATP-bd.
IPR019805. Heat_shock_protein_90_CS.
IPR001404. Hsp90_fam.
IPR020575. Hsp90_N.
IPR020568. Ribosomal_S5_D2-typ_fold.
[Graphical view ]
PANTHERi PTHR11528. PTHR11528. 1 hit.
Pfami PF00183. HSP90. 1 hit.
[Graphical view ]
PIRSFi PIRSF002583. Hsp90. 1 hit.
PRINTSi PR00775. HEATSHOCK90.
SMARTi SM00387. HATPase_c. 1 hit.
[Graphical view ]
SUPFAMi SSF54211. SSF54211. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEi PS00014. ER_TARGET. 1 hit.
PS00298. HSP90. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A protein kinase isolated from porcine brain microvessels is similar to a class of heat-shock proteins."
    Dechert U., Weber P., Koenig B., Ortwein C., Nilson I., Linxweiler W., Wollny E., Gassen H.G.
    Eur. J. Biochem. 225:805-809(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Structurally related high density lipoprotein-binding proteins in liver I: identification as gp96/GRP94."
    de Crom R.P.G., van Haperen R., Janssens R., Visser P., van der Kamp A.W.M.
    Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  3. "Cloning and characterization of a porcine protein kinase gene and relationship to a class of heat shock proteins."
    Koenig B., Seehaus B., Bangsow T., Bangsow T., Henninger J., Weber P., Schepelmann S., Wollny E., Gassen H.G.
    DNA Cell Biol. 16:1365-1372(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Liver.

Entry informationi

Entry nameiENPL_PIG
AccessioniPrimary (citable) accession number: Q29092
Secondary accession number(s): O19070, Q29091
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: December 13, 2001
Last modified: June 11, 2014
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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