Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q29092

- ENPL_PIG

UniProt

Q29092 - ENPL_PIG

Protein

Endoplasmin

Gene

HSP90B1

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 3 (13 Dec 2001)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Molecular chaperone that functions in the processing and transport of secreted proteins. When associated with CNPY3, required for proper folding of Toll-like receptors. Functions in endoplasmic reticulum associated degradation (ERAD). Has ATPase activity By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei107 – 1071ATPBy similarity
    Binding sitei149 – 1491ATPBy similarity
    Binding sitei162 – 1621ATPBy similarity
    Binding sitei168 – 1681ATPBy similarity
    Binding sitei199 – 1991ATP; via amide nitrogenBy similarity
    Binding sitei448 – 4481ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. ER-associated ubiquitin-dependent protein catabolic process Source: UniProtKB
    2. protein folding Source: InterPro
    3. response to stress Source: InterPro

    Keywords - Molecular functioni

    Chaperone

    Keywords - Ligandi

    ATP-binding, Calcium, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Endoplasmin
    Alternative name(s):
    94 kDa glucose-regulated protein
    Short name:
    GRP-94
    98 kDa protein kinase
    Short name:
    PPK 98
    Short name:
    ppk98
    Heat shock protein 90 kDa beta member 1
    gp96 homolog
    Gene namesi
    Name:HSP90B1
    Synonyms:GRP94, TRA1
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. endoplasmic reticulum lumen Source: UniProtKB-SubCell
    2. melanosome Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Endoplasmic reticulum

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2121Sequence AnalysisAdd
    BLAST
    Chaini22 – 804783EndoplasminPRO_0000013600Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi62 – 621N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi107 – 1071N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi138 – 138InterchainBy similarity
    Glycosylationi217 – 2171N-linked (GlcNAc...)Sequence Analysis
    Modified residuei306 – 3061PhosphoserineBy similarity
    Modified residuei404 – 4041N6-succinyllysineBy similarity
    Glycosylationi445 – 4451N-linked (GlcNAc...)Sequence Analysis
    Modified residuei479 – 4791N6-acetyllysineBy similarity
    Glycosylationi481 – 4811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi502 – 5021N-linked (GlcNAc...)Sequence Analysis
    Modified residuei633 – 6331N6-succinyllysineBy similarity

    Post-translational modificationi

    Phosphorylated.By similarity

    Keywords - PTMi

    Acetylation, Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiQ29092.
    PRIDEiQ29092.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5 By similarity. Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with AIMP1; regulates its retention in the endoplasmic reticulum. Interacts with OS9 By similarity. Interacts with CNPY3; this interaction is disrupted in the presence of ATP. Interacts with several TLRs, including TLR4 and TLR9, but not with TLR3 By similarity. Interacts with MZB1 in a calcium-dependent manner By similarity. Interacts with METTL23 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ29092.
    SMRiQ29092. Positions 69-755.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi801 – 8044Prevents secretion from ERSequence Analysis

    Sequence similaritiesi

    Belongs to the heat shock protein 90 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG0326.
    HOGENOMiHOG000031988.
    HOVERGENiHBG007374.
    KOiK09487.

    Family and domain databases

    Gene3Di3.30.565.10. 2 hits.
    HAMAPiMF_00505. HSP90.
    InterProiIPR003594. HATPase_ATP-bd.
    IPR019805. Heat_shock_protein_90_CS.
    IPR001404. Hsp90_fam.
    IPR020575. Hsp90_N.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    [Graphical view]
    PANTHERiPTHR11528. PTHR11528. 1 hit.
    PfamiPF00183. HSP90. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002583. Hsp90. 1 hit.
    PRINTSiPR00775. HEATSHOCK90.
    SMARTiSM00387. HATPase_c. 1 hit.
    [Graphical view]
    SUPFAMiSSF54211. SSF54211. 1 hit.
    SSF55874. SSF55874. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    PS00298. HSP90. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q29092-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRALWVLGLC CVLLTFGSVR AEDEVDVDGT VEEDLGKSRE GSRTDDEVVQ    50
    REEEAIQLDG LNASQIRELR EKSEKFAFQA EVNRMMKLII NSLYKNKEIF 100
    LRELISNASD ALDKIRLISL TDENALAGNE ELTVKIKCDK EKNLLHVTDT 150
    GVGMTREELV KNLGTIAKSG TSEFLNKMAE AQEDGQSTSE LIGQFGVGFY 200
    SAFLVADKVI VTSKHNNDTQ HIWESDSNEF SVIADPRGNT LGRGTTITLV 250
    LKEEASDYLE LDTIKNLVKK YSQFINFPIY VWSSKTETVE EPMEEEEAAK 300
    EEKEESDDEA AVEEEEEEKK PKTKKVEKTV WDWELMNDIK PIWQRPSKEV 350
    EDDEYKAFYK SFSKESDDPM AYIHFTAEGE VTFKSILFVP TSAPRGLFDE 400
    YGSKKSDYIK LYVRRVFITD DFHDMMPKYL NFVKGVVDSD DLPLNVSRET 450
    LQQHKLLKVI RKKLVRKTLD MIKKIADEKY NDTFWKEFGT NIKLGVIEDH 500
    SNRTRLAKLL RFQSSHHPSD ITSLDQYVER MKEKQDKIYF MAGSSRKEAE 550
    SSPFVERLLK KGYEVIYLTE PVDEYCIQAL PEFDGKRFQN VAKEGVKFDE 600
    SEKSKENREA VEKEFEPLLN WMKDKALKDK IEKAVVSQRL TESPCALVAS 650
    QYGWSGNMER IMKAQAYQTG KDISTNYYAS QKKTFEINPR HPLIRDMLRR 700
    VKEDEDDKTV SDLAVVLFET ATLRSGYLLP DTKAYGDRIE RMLRLSLNID 750
    PDAKVEEEPE EEPEETTEDT TEDTEQDDDE EMDAGADEEE QETSETSTAE 800
    KDEL 804
    Length:804
    Mass (Da):92,471
    Last modified:December 13, 2001 - v3
    Checksum:iFF823A77406F0190
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti48 – 481V → I in CAA70347. 1 PublicationCurated
    Sequence conflicti179 – 1791A → T in CAA70347. 1 PublicationCurated
    Sequence conflicti332 – 3343DWE → ELG in CAA62352. (PubMed:9407008)Curated
    Sequence conflicti795 – 7951E → EFVFQ in CAA62352. (PubMed:9407008)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76301 mRNA. Translation: CAA53948.1.
    Y09136 mRNA. Translation: CAA70347.1.
    X90848 Genomic DNA. Translation: CAA62352.1.
    PIRiPL0137.
    S51358.
    RefSeqiNP_999268.1. NM_214103.1.
    UniGeneiSsc.94181.
    Ssc.9889.

    Genome annotation databases

    GeneIDi397191.
    KEGGissc:397191.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76301 mRNA. Translation: CAA53948.1 .
    Y09136 mRNA. Translation: CAA70347.1 .
    X90848 Genomic DNA. Translation: CAA62352.1 .
    PIRi PL0137.
    S51358.
    RefSeqi NP_999268.1. NM_214103.1.
    UniGenei Ssc.94181.
    Ssc.9889.

    3D structure databases

    ProteinModelPortali Q29092.
    SMRi Q29092. Positions 69-755.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi Q29092.
    PRIDEi Q29092.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 397191.
    KEGGi ssc:397191.

    Organism-specific databases

    CTDi 7184.

    Phylogenomic databases

    eggNOGi COG0326.
    HOGENOMi HOG000031988.
    HOVERGENi HBG007374.
    KOi K09487.

    Miscellaneous databases

    PROi Q29092.

    Family and domain databases

    Gene3Di 3.30.565.10. 2 hits.
    HAMAPi MF_00505. HSP90.
    InterProi IPR003594. HATPase_ATP-bd.
    IPR019805. Heat_shock_protein_90_CS.
    IPR001404. Hsp90_fam.
    IPR020575. Hsp90_N.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    [Graphical view ]
    PANTHERi PTHR11528. PTHR11528. 1 hit.
    Pfami PF00183. HSP90. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002583. Hsp90. 1 hit.
    PRINTSi PR00775. HEATSHOCK90.
    SMARTi SM00387. HATPase_c. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54211. SSF54211. 1 hit.
    SSF55874. SSF55874. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    PS00298. HSP90. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A protein kinase isolated from porcine brain microvessels is similar to a class of heat-shock proteins."
      Dechert U., Weber P., Koenig B., Ortwein C., Nilson I., Linxweiler W., Wollny E., Gassen H.G.
      Eur. J. Biochem. 225:805-809(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Structurally related high density lipoprotein-binding proteins in liver I: identification as gp96/GRP94."
      de Crom R.P.G., van Haperen R., Janssens R., Visser P., van der Kamp A.W.M.
      Submitted (OCT-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Liver.
    3. "Cloning and characterization of a porcine protein kinase gene and relationship to a class of heat shock proteins."
      Koenig B., Seehaus B., Bangsow T., Bangsow T., Henninger J., Weber P., Schepelmann S., Wollny E., Gassen H.G.
      DNA Cell Biol. 16:1365-1372(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Liver.

    Entry informationi

    Entry nameiENPL_PIG
    AccessioniPrimary (citable) accession number: Q29092
    Secondary accession number(s): O19070, Q29091
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: December 13, 2001
    Last modified: October 1, 2014
    This is version 121 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3