ID   PO2F1_PIG               Reviewed;         745 AA.
AC   Q29076;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   24-JAN-2024, entry version 136.
DE   RecName: Full=POU domain, class 2, transcription factor 1;
DE   AltName: Full=NF-A1;
DE   AltName: Full=Octamer-binding protein 1;
DE            Short=Oct-1;
DE   AltName: Full=Octamer-binding transcription factor 1;
DE            Short=OTF-1;
GN   Name=POU2F1; Synonyms=OTF1;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Brain, and Spleen;
RX   PubMed=8535082; DOI=10.1007/bf00352381;
RA   Tuggle C.K., Schmitz C.B., Wang L., Rothschild M.F.;
RT   "Cloning and mapping of porcine OTF1 extends a synteny group conserved on
RT   SSC 4 and HSA 1.";
RL   Mamm. Genome 6:673-676(1995).
CC   -!- FUNCTION: Transcription factor that binds to the octamer motif (5'-
CC       ATTTGCAT-3') and activates the promoters of the genes for some small
CC       nuclear RNAs (snRNA) and of genes such as those for histone H2B and
CC       immunoglobulins. Modulates transcription transactivation by NR3C1, AR
CC       and PGR. {ECO:0000250|UniProtKB:P14859}.
CC   -!- SUBUNIT: Interacts with POU2AF1; the interaction increases POU2F1
CC       transactivation activity. Interacts with NR3C1, AR, PGR and HCFC1.
CC       {ECO:0000250|UniProtKB:P14859}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC       ECO:0000255|PROSITE-ProRule:PRU00530}.
CC   -!- PTM: Phosphorylated by PRKDC. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the POU transcription factor family. Class-2
CC       subfamily. {ECO:0000305}.
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DR   EMBL; L38524; AAA93523.1; -; mRNA.
DR   RefSeq; NP_999429.1; NM_214264.1.
DR   AlphaFoldDB; Q29076; -.
DR   SMR; Q29076; -.
DR   STRING; 9823.ENSSSCP00000058676; -.
DR   PaxDb; 9823-ENSSSCP00000006729; -.
DR   GeneID; 397501; -.
DR   KEGG; ssc:397501; -.
DR   CTD; 5451; -.
DR   eggNOG; KOG3802; Eukaryota.
DR   InParanoid; Q29076; -.
DR   OrthoDB; 4250502at2759; -.
DR   Proteomes; UP000008227; Unplaced.
DR   Proteomes; UP000314985; Unplaced.
DR   Proteomes; UP000694570; Unplaced.
DR   Proteomes; UP000694571; Unplaced.
DR   Proteomes; UP000694720; Unplaced.
DR   Proteomes; UP000694722; Unplaced.
DR   Proteomes; UP000694723; Unplaced.
DR   Proteomes; UP000694724; Unplaced.
DR   Proteomes; UP000694725; Unplaced.
DR   Proteomes; UP000694726; Unplaced.
DR   Proteomes; UP000694727; Unplaced.
DR   Proteomes; UP000694728; Unplaced.
DR   GO; GO:0005634; C:nucleus; IC:AgBase.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IC:AgBase.
DR   GO; GO:0045892; P:negative regulation of DNA-templated transcription; IMP:AgBase.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   Gene3D; 1.10.260.40; lambda repressor-like DNA-binding domains; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR013847; POU.
DR   InterPro; IPR045703; POU2F1_C.
DR   InterPro; IPR000327; POU_dom.
DR   InterPro; IPR000972; TF_octamer.
DR   PANTHER; PTHR11636; POU DOMAIN; 1.
DR   PANTHER; PTHR11636:SF47; POU DOMAIN, CLASS 2, TRANSCRIPTION FACTOR 1; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF00157; Pou; 1.
DR   Pfam; PF19536; POU2F1_C; 1.
DR   PRINTS; PR00029; OCTAMER.
DR   PRINTS; PR00028; POUDOMAIN.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00352; POU; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   SUPFAM; SSF47413; lambda repressor-like DNA-binding domains; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS00035; POU_1; 1.
DR   PROSITE; PS00465; POU_2; 1.
DR   PROSITE; PS51179; POU_3; 1.
PE   2: Evidence at transcript level;
KW   Activator; DNA-binding; Homeobox; Nucleus; Phosphoprotein;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..745
FT                   /note="POU domain, class 2, transcription factor 1"
FT                   /id="PRO_0000100710"
FT   DOMAIN          280..354
FT                   /note="POU-specific"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT   DNA_BIND        381..440
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          1..34
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          67..95
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          497..559
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..29
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         270
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:P14859"
FT   MOD_RES         276
FT                   /note="Phosphothreonine"
FT                   /evidence="ECO:0000250|UniProtKB:P14859"
FT   MOD_RES         283
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P14859"
FT   MOD_RES         387
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P14859"
FT   MOD_RES         450
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P14859"
SQ   SEQUENCE   745 AA;  76596 MW;  83564E9CC03741BA CRC64;
     MNNPSETSKP SMESGDSNTG TQTNGLDFQK QPVPVGGAIS TAQAQAFLGH LHQVQLAGTS
     LQAAAQSLNV QSKSNEESGD SQQPSQPSQQ PSVQAAIPQT QLMLAGGQIT GLTLTPAQQQ
     LLLQQAQAQA QLLAAAVQQH SASQQHSAAG ATISASAATP MTQIPLSQPI QIAQDLQQLQ
     QLQQQNLNLQ QFVLGHPTTN LQPAQFIISQ TPQGQQGLLQ AQNLLTQLPQ QSQANLLQSQ
     PSITLTSQPA TPTRTIAATP IQTLPQSQST PKRIDTPSLE EPSDLEELEQ FAKTFKQRRI
     KLGFTQGDVG LAMGKLYGND FSQTTISRFE ALNLSFKNMC KLKPLLEKWL NDAENLSSDS
     ALCSPSALNS PGLGVEGLNR RRKKRTSIET NIRVALEKSF LENQKPTSEE ITMIADQLNM
     EKEVIRVWFC NRRQKEKRIN PPSSGGTSSS PIKAIFPSPT SLVGTTPSLV TSSAATTLTV
     NPVLPLTSAA VTNLSVTGTT DTTSNNTATV ISTAPPASSA VTSPSLSPSP SASGSTSEVS
     SASETSTTQT TSTPLSSPLG TSQVMVTASG LQTAAAAALQ GAAQLPANAS LAAMAAAAGL
     NPGLMAPSQF AAGGALLSLN PGTLGGALSP ALMSNSTLAT IQALASGGSL PITSLDATGN
     LVFGNAGGAP NIVTAPLFLN PQNLSLLTSN PVSLVSAAAA SAGNSGPVAS LHATSTSAES
     IQNSLFTVAS ASGAASTTTT ASKAQ
//