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Protein

Antimicrobial peptide NK-lysin

Gene

NKL

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May be an effector molecule of cytotoxic activity. High activity against E.coli and B.megaterium, moderate against A.calcoaceticus and S.pyogenes. No activity against P.aeruginosa, S.aureus and Salmonella. Has some antifungal activity against C.albicans.

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Antibiotic, Antimicrobial, Fungicide

Protein family/group databases

TCDBi1.C.35.3.1. the amoebapore (amoebapore) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Antimicrobial peptide NK-lysin
Short name:
NKL
Gene namesi
Name:NKL
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei‹1 – 6›6Sequence analysis
Propeptidei7 – 46401 PublicationPRO_0000031663Add
BLAST
Peptidei47 – 12478Antimicrobial peptide NK-lysinPRO_0000031664Add
BLAST
Propeptidei125 – 1295PRO_0000031665

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi50 ↔ 122
Disulfide bondi53 ↔ 116
Disulfide bondi81 ↔ 91

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ29075.
PeptideAtlasiQ29075.

Expressioni

Tissue specificityi

Cytotoxic T and NK cells.

Inductioni

By interleukin-2.

Interactioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000008784.

Structurei

Secondary structure

1
129
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi49 – 6416Combined sources
Helixi70 – 8314Combined sources
Helixi88 – 10720Combined sources
Helixi112 – 1187Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NKLNMR-A47-124[»]
ProteinModelPortaliQ29075.
SMRiQ29075. Positions 47-124.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ29075.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 12681Saposin B-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 saposin B-type domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410J70I. Eukaryota.
ENOG41117YV. LUCA.
HOGENOMiHOG000112748.
HOVERGENiHBG005866.
InParanoidiQ29075.

Family and domain databases

Gene3Di1.10.225.10. 1 hit.
InterProiIPR007856. SapB_1.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
[Graphical view]
PfamiPF05184. SapB_1. 1 hit.
[Graphical view]
SMARTiSM00741. SapB. 1 hit.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 1 hit.
PROSITEiPS50015. SAP_B. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q29075-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
PGLAFSGLTP EHSALARAHP CDGEQFCQNL APEDPQGDQL LQREELGLIC
60 70 80 90 100
ESCRKIIQKL EDMVGPQPNE DTVTQAASRV CDKMKILRGV CKKIMRTFLR
110 120
RISKDILTGK KPQAICVDIK ICKEKTGLI
Length:129
Mass (Da):14,317
Last modified:November 1, 1996 - v1
Checksum:i4A87B206E20C53AD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti48 – 492LI → YF.
Natural varianti79 – 791R → Q.
Natural varianti84 – 841M → L.
Natural varianti90 – 901V → L.
Natural varianti97 – 971T → S.
Natural varianti104 – 1041K → W.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85431 mRNA. Translation: CAA59720.1.
PIRiS55044.
UniGeneiSsc.16227.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X85431 mRNA. Translation: CAA59720.1.
PIRiS55044.
UniGeneiSsc.16227.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NKLNMR-A47-124[»]
ProteinModelPortaliQ29075.
SMRiQ29075. Positions 47-124.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000008784.

Protein family/group databases

TCDBi1.C.35.3.1. the amoebapore (amoebapore) family.

Proteomic databases

PaxDbiQ29075.
PeptideAtlasiQ29075.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG410J70I. Eukaryota.
ENOG41117YV. LUCA.
HOGENOMiHOG000112748.
HOVERGENiHBG005866.
InParanoidiQ29075.

Miscellaneous databases

EvolutionaryTraceiQ29075.

Family and domain databases

Gene3Di1.10.225.10. 1 hit.
InterProiIPR007856. SapB_1.
IPR011001. Saposin-like.
IPR008139. SaposinB_dom.
[Graphical view]
PfamiPF05184. SapB_1. 1 hit.
[Graphical view]
SMARTiSM00741. SapB. 1 hit.
[Graphical view]
SUPFAMiSSF47862. SSF47862. 1 hit.
PROSITEiPS50015. SAP_B. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "NK-lysin, a novel effector peptide of cytotoxic T and NK cells. Structure and cDNA cloning of the porcine form, induction by interleukin 2, antibacterial and antitumour activity."
    Andersson M., Gunne H., Agerberth B., Boman A., Bergman T., Sillard R., Joernvall H., Mutt V., Olsson B., Wigzell H., Dagerlind A., Boman H.G., Gudmundsson G.H.
    EMBO J. 14:1615-1625(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 47-124.
    Tissue: Bone marrow and Small intestine.
  2. "Saposin fold revealed by the NMR structure of NK-lysin."
    Liepinsh E., Andersson M., Ruysschaert J.-M., Otting G.
    Nat. Struct. Biol. 4:793-795(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 47-124.

Entry informationi

Entry nameiNKL_PIG
AccessioniPrimary (citable) accession number: Q29075
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1996
Last modified: July 6, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.