Reviewed, UniProtKB/Swiss-Prot Q29043 (FUT1_PIG)

Names and origin

Protein names

Recommended name:
    Galactoside 2-alpha-L-fucosyltransferase 1
    EC=2.4.1.69
Alternative name(s):
    GDP-L-fucose:beta-D-galactoside 2-alpha-L-fucosyltransferase 1
    Alpha(1,2)FT 1
    Fucosyltransferase 1

Gene names

Name:

FUT1

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	GDP-beta-L-fucose + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = GDP + alpha-L-fucosyl-(1->2)-beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein. Note: Membrane-bound form in trans cisternae of Golgi.
Miscellaneous	There are two genes (Fut1 and Fut2) which encode galactoside 2-L-fucosyltransferase. They are expressed in a tissue-specific manner.
Sequence similarities	Belongs to the glycosyltransferase 11 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Domain	Signal-anchor Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	galactoside 2-alpha-L-fucosyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Galactoside 2-alpha-L-fucosyltransferase 1		PRO_0000149100
Regions
	Topological domain	1 – 8	8	Cytoplasmic Potential
	Transmembrane	9 – 28	20	Signal-anchor for type II membrane protein Potential
	Topological domain	29 – 365	337	Lumenal Potential
Amino acid modifications
	Glycosylation	65	1	N-linked (GlcNAc...) Potential
	Glycosylation	301	1	N-linked (GlcNAc...) Potential
	Glycosylation	327	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	103	1	A → T
	Natural variant	286	1	R → E
Experimental info
	Sequence conflict	77	1	L → F in AAB02984. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q29043-1 [UniParc]. Last modified December 1, 2000. Version 2. Checksum: DAFCE77E89A29D75	FASTA	365	41,106
	10 20 30 40 50 60 MWVPSRRHLC LTFLLVCVLA AIFFLNVYQD LFYSGLDLLA LCPDHNVVSS PVAIFCLAGT 70 80 90 100 110 120 PVHPNASDSC PKHPASLSGT WTIYPDGRFG NQMGQYATLL ALAQLNGRQA FIQPAMHAVL 130 140 150 160 170 180 APVFRITLPV LAPEVDRHAP WRELELHDWM SEDYAHLKEP WLKLTGFPCS WTFFHHLREQ 190 200 210 220 230 240 IRSEFTLHDH LRQEAQGVLS QFRLPRTGDR PSTFVGVHVR RGDYLRVMPK RWKGVVGDGA 250 260 270 280 290 300 YLQQAMDWFR ARYEAPVFVV TSNGMEWCRK NIDTSRGDVI FAGDGREAAP ARDFALLVQC 310 320 330 340 350 360 NHTIMTIGTF GFWAAYLAGG DTIYLANFTL PTSSFLKIFK PEAAFLPEWV GINADLSPLQ MLAGP

References

[1]	"Molecular cloning of the gene coding for pig alpha1-->2 fucosyltransferase." Cohney S., Mouhtouris E., McKenzie I.F., Sandrin M.S. Immunogenetics 44:76-79(1996) [PubMed: 8613146] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Two alpha(1,2) fucosyltransferase genes on porcine chromosome 6q11 are closely linked to the blood group inhibitor (S) and Escherichia coli F18 receptor (ECF18R) loci." Meijerink E., Fries R., Voegeli P., Masabanda J., Wigger G., Stricker C., Neuenschwander S., Bertschinger H.U., Stranzinger G. Mamm. Genome 8:736-741(1997) [PubMed: 9321466] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANTS THR-103 AND GLU-286.
[3]	"A DNA polymorphism influencing alpha(1,2)fucosyltransferase activity of the pig FUT1 enzyme determines susceptibility of small intestinal epithelium to Escherichia coli F18 adhesion." Meijerink E., Neuenschwander S., Fries R., Dinter A., Bertschinger H.U., Stranzinger G., Vogeli P. Immunogenetics 52:129-136(2000) [PubMed: 11132149] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], VARIANTS THR-103 AND GLU-286. Tissue: Small intestine.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L50534 Genomic DNA. Translation: AAB02984.1. U70883 Genomic DNA. Translation: AAB81884.1. AF136896 mRNA. Translation: AAF59833.1.
RefSeq	NP_999233.1.
UniGene	Ssc.242
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT11. Glycosyltransferase Family 11.
Genome annotation databases
GeneID	397138.
KEGG	ssc:397138.
Organism-specific databases
CTD	397138.
Phylogenomic databases
HOVERGEN	Q29043.
Enzyme and pathway databases
BRENDA	2.4.1.69. 249.
Family and domain databases
InterPro	IPR002516. Glyco_trans_11. [Graphical view]
PANTHER	PTHR11927. Glyco_trans_11. 1 hit.
Pfam	PF01531. Glyco_transf_11. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUT1_PIG

Accession

Primary (citable) accession number: Q29043
Secondary accession number(s): O19101

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1997
Last sequence update:	December 1, 2000
Last modified:	October 13, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)