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Q29029

- MPIP3_PIG

UniProt

Q29029 - MPIP3_PIG

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Protein

M-phase inducer phosphatase 3

Gene
CDC25C
Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. Directly dephosphorylates CDK1 and activates its kinase activity. When phosphorylated, highly effective in activating G2 cells into prophase By similarity.

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei406 – 4061 By similarity

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. G2/M transition of mitotic cell cycle Source: Ensembl
  2. mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiREACT_106629. Cyclin B2 mediated events.
REACT_193437. Polo-like kinase mediated events.
REACT_218303. Activation of ATR in response to replication stress.

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase 3 (EC:3.1.3.48)
Alternative name(s):
Dual specificity phosphatase Cdc25C
Gene namesi
Name:CDC25C
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Chromosome 2

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502M-phase inducer phosphatase 3PRO_0000198650Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei30 – 301Phosphoserine By similarity
Modified residuei40 – 401Phosphothreonine; by CDK1 By similarity
Modified residuei50 – 501Phosphoserine By similarity
Modified residuei54 – 541Phosphoserine By similarity
Modified residuei57 – 571Phosphoserine By similarity
Modified residuei60 – 601Phosphothreonine; by CDK1 By similarity
Modified residuei115 – 1151Phosphoserine; by CDK1 By similarity
Modified residuei122 – 1221Phosphoserine By similarity
Modified residuei123 – 1231Phosphothreonine; by CDK1 By similarity
Modified residuei194 – 1941Phosphoserine; by CDK1 By similarity
Modified residuei217 – 2171Phosphoserine; by PLK3 By similarity
Modified residuei224 – 2241Phosphoserine; by PLK3 By similarity
Modified residuei243 – 2431Phosphoserine; by CDK1 By similarity
Modified residuei245 – 2451Phosphoserine; by CHEK1, CHEK2, BRSK1 and MAPK14 By similarity
Modified residuei501 – 5011Phosphoserine By similarity

Post-translational modificationi

Phosphorylated by CHEK1 and MAPK14 at Ser-245. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase By similarity. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-217 promotes nuclear translocation. Ser-224 is a minor phosphorylation site By similarity. Phosphorylation by CDK1 occurs at G2 and G2-M transition and leads to increased activity By similarity.

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Interacts with MAPK14 and 14-3-3 proteins By similarity. When phosphorylated at Ser-122 and/or Thr-123, interacts with PLK1 By similarity.

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000015243.

Structurei

3D structure databases

ProteinModelPortaliQ29029.
SMRiQ29029. Positions 311-473.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini350 – 457108RhodaneseAdd
BLAST

Sequence similaritiesi

Belongs to the MPI phosphatase family.
Contains 1 rhodanese domain.

Phylogenomic databases

eggNOGiCOG5105.
GeneTreeiENSGT00390000018747.
HOGENOMiHOG000082672.
HOVERGENiHBG052501.
KOiK05867.
OMAiTVSLCDI.
OrthoDBiEOG7288R1.
TreeFamiTF101056.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q29029-1 [UniParc]FASTAAdd to Basket

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MAEGGRPGSG PSFRSNHRKI LNLLLERETS FSMSSDRPGT PLKKKLFGDS    50
ANLSILSGGT PKRCLDLSNL SSGEMSATQF TTSADLDETG HLDSTGSEEI 100
QLAGMSYHQH LLKCSPAQLL CSTPNALDHG RRKKDAICSS SENKENENNT 150
SKPLEWWAHR NLLFQKRPGG PYMSPLSLLD NGNLVEGEMK HLGSPITAVS 200
KLDKNPERGE DQAEEISDEL MEFSLEDQEE AKVSLNTSCL YRSFSLPDSL 250
NSPGLKQVVK FKDHTLPDKV KKKYCSSHEE LRKGLGVKKM VSLCNINMIQ 300
MLEEDSNQGP LIGDFSKVCA LPTVSGRHQD LKYVNPETVA ALLSGKFQGL 350
IEKFYIIDCR YPYEYLGGHI QGALNLYSQE ELCNFFLKKP IIPLDTQKRI 400
IVVFHCEFSS ERGPRMCRSL REEDRALNQY PALYYPELYI LKGGYRDFFP 450
EYMELCEPQS YCPMHHQDHK AELLRCRSQN KAWEGERQLQ EQIALLVKDV 500
SP 502
Length:502
Mass (Da):56,639
Last modified:November 1, 1997 - v1
Checksum:i02E28CE42C0EDA70
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78317 Genomic DNA. Translation: CAA55123.1.
PIRiS43123.
RefSeqiNP_001116567.1. NM_001123095.1.
XP_005661723.1. XM_005661666.1.
XP_005661724.1. XM_005661667.1.
XP_005661725.1. XM_005661668.1.
XP_005661726.1. XM_005661669.1.
UniGeneiSsc.26311.

Genome annotation databases

EnsembliENSSSCT00000015655; ENSSSCP00000015242; ENSSSCG00000014329.
GeneIDi100144474.
KEGGissc:100144474.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78317 Genomic DNA. Translation: CAA55123.1 .
PIRi S43123.
RefSeqi NP_001116567.1. NM_001123095.1.
XP_005661723.1. XM_005661666.1.
XP_005661724.1. XM_005661667.1.
XP_005661725.1. XM_005661668.1.
XP_005661726.1. XM_005661669.1.
UniGenei Ssc.26311.

3D structure databases

ProteinModelPortali Q29029.
SMRi Q29029. Positions 311-473.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000015243.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSSSCT00000015655 ; ENSSSCP00000015242 ; ENSSSCG00000014329 .
GeneIDi 100144474.
KEGGi ssc:100144474.

Organism-specific databases

CTDi 995.

Phylogenomic databases

eggNOGi COG5105.
GeneTreei ENSGT00390000018747.
HOGENOMi HOG000082672.
HOVERGENi HBG052501.
KOi K05867.
OMAi TVSLCDI.
OrthoDBi EOG7288R1.
TreeFami TF101056.

Enzyme and pathway databases

Reactomei REACT_106629. Cyclin B2 mediated events.
REACT_193437. Polo-like kinase mediated events.
REACT_218303. Activation of ATR in response to replication stress.

Family and domain databases

Gene3Di 3.40.250.10. 1 hit.
InterProi IPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view ]
Pfami PF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view ]
PRINTSi PR00716. MPIPHPHTASE.
SMARTi SM00450. RHOD. 1 hit.
[Graphical view ]
SUPFAMi SSF52821. SSF52821. 1 hit.
PROSITEi PS50206. RHODANESE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of a pig cdc25 tyrosine phosphatase cDNA."
    Cui H., Xu W., Powell D., Newman B.J.
    J. Anim. Sci. 73:630-630(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Ovary.

Entry informationi

Entry nameiMPIP3_PIG
AccessioniPrimary (citable) accession number: Q29029
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: September 3, 2014
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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