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Q29029

- MPIP3_PIG

UniProt

Q29029 - MPIP3_PIG

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Protein

M-phase inducer phosphatase 3

Gene

CDC25C

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. Directly dephosphorylates CDK1 and activates its kinase activity. When phosphorylated, highly effective in activating G2 cells into prophase By similarity.By similarity

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei406 – 4061By similarity

GO - Molecular functioni

  1. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. G2/M transition of mitotic cell cycle Source: Ensembl
  2. mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiREACT_106629. Cyclin B2 mediated events.
REACT_193437. Polo-like kinase mediated events.
REACT_218303. Activation of ATR in response to replication stress.

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase 3 (EC:3.1.3.48)
Alternative name(s):
Dual specificity phosphatase Cdc25C
Gene namesi
Name:CDC25C
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
ProteomesiUP000008227: Chromosome 2

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. cytoplasm Source: Ensembl
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 502502M-phase inducer phosphatase 3PRO_0000198650Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei30 – 301PhosphoserineBy similarity
Modified residuei40 – 401Phosphothreonine; by CDK1By similarity
Modified residuei50 – 501PhosphoserineBy similarity
Modified residuei54 – 541PhosphoserineBy similarity
Modified residuei57 – 571PhosphoserineBy similarity
Modified residuei60 – 601Phosphothreonine; by CDK1By similarity
Modified residuei115 – 1151Phosphoserine; by CDK1By similarity
Modified residuei122 – 1221PhosphoserineBy similarity
Modified residuei123 – 1231Phosphothreonine; by CDK1By similarity
Modified residuei194 – 1941Phosphoserine; by CDK1By similarity
Modified residuei217 – 2171Phosphoserine; by PLK3By similarity
Modified residuei224 – 2241Phosphoserine; by PLK3By similarity
Modified residuei243 – 2431Phosphoserine; by CDK1By similarity
Modified residuei245 – 2451Phosphoserine; by CHEK1, CHEK2, BRSK1 and MAPK14By similarity
Modified residuei501 – 5011PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated by CHEK1 and MAPK14 at Ser-245. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase By similarity. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-217 promotes nuclear translocation. Ser-224 is a minor phosphorylation site By similarity. Phosphorylation by CDK1 occurs at G2 and G2-M transition and leads to increased activity By similarity.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Interacts with MAPK14 and 14-3-3 proteins. When phosphorylated at Ser-122 and/or Thr-123, interacts with PLK1.By similarity

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000015243.

Structurei

3D structure databases

ProteinModelPortaliQ29029.
SMRiQ29029. Positions 311-473.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini350 – 457108RhodanesePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the MPI phosphatase family.Curated
Contains 1 rhodanese domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5105.
GeneTreeiENSGT00390000018747.
HOGENOMiHOG000082672.
HOVERGENiHBG052501.
InParanoidiQ29029.
KOiK05867.
OMAiTVSLCDI.
OrthoDBiEOG7288R1.
TreeFamiTF101056.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PfamiPF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSiPR00716. MPIPHPHTASE.
SMARTiSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiPS50206. RHODANESE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q29029-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAEGGRPGSG PSFRSNHRKI LNLLLERETS FSMSSDRPGT PLKKKLFGDS
60 70 80 90 100
ANLSILSGGT PKRCLDLSNL SSGEMSATQF TTSADLDETG HLDSTGSEEI
110 120 130 140 150
QLAGMSYHQH LLKCSPAQLL CSTPNALDHG RRKKDAICSS SENKENENNT
160 170 180 190 200
SKPLEWWAHR NLLFQKRPGG PYMSPLSLLD NGNLVEGEMK HLGSPITAVS
210 220 230 240 250
KLDKNPERGE DQAEEISDEL MEFSLEDQEE AKVSLNTSCL YRSFSLPDSL
260 270 280 290 300
NSPGLKQVVK FKDHTLPDKV KKKYCSSHEE LRKGLGVKKM VSLCNINMIQ
310 320 330 340 350
MLEEDSNQGP LIGDFSKVCA LPTVSGRHQD LKYVNPETVA ALLSGKFQGL
360 370 380 390 400
IEKFYIIDCR YPYEYLGGHI QGALNLYSQE ELCNFFLKKP IIPLDTQKRI
410 420 430 440 450
IVVFHCEFSS ERGPRMCRSL REEDRALNQY PALYYPELYI LKGGYRDFFP
460 470 480 490 500
EYMELCEPQS YCPMHHQDHK AELLRCRSQN KAWEGERQLQ EQIALLVKDV

SP
Length:502
Mass (Da):56,639
Last modified:November 1, 1997 - v1
Checksum:i02E28CE42C0EDA70
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78317 Genomic DNA. Translation: CAA55123.1.
PIRiS43123.
RefSeqiNP_001116567.1. NM_001123095.1.
XP_005661723.1. XM_005661666.1.
XP_005661724.1. XM_005661667.1.
XP_005661725.1. XM_005661668.1.
XP_005661726.1. XM_005661669.1.
UniGeneiSsc.26311.

Genome annotation databases

EnsembliENSSSCT00000015655; ENSSSCP00000015242; ENSSSCG00000014329.
GeneIDi100144474.
KEGGissc:100144474.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78317 Genomic DNA. Translation: CAA55123.1 .
PIRi S43123.
RefSeqi NP_001116567.1. NM_001123095.1.
XP_005661723.1. XM_005661666.1.
XP_005661724.1. XM_005661667.1.
XP_005661725.1. XM_005661668.1.
XP_005661726.1. XM_005661669.1.
UniGenei Ssc.26311.

3D structure databases

ProteinModelPortali Q29029.
SMRi Q29029. Positions 311-473.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 9823.ENSSSCP00000015243.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSSSCT00000015655 ; ENSSSCP00000015242 ; ENSSSCG00000014329 .
GeneIDi 100144474.
KEGGi ssc:100144474.

Organism-specific databases

CTDi 995.

Phylogenomic databases

eggNOGi COG5105.
GeneTreei ENSGT00390000018747.
HOGENOMi HOG000082672.
HOVERGENi HBG052501.
InParanoidi Q29029.
KOi K05867.
OMAi TVSLCDI.
OrthoDBi EOG7288R1.
TreeFami TF101056.

Enzyme and pathway databases

Reactomei REACT_106629. Cyclin B2 mediated events.
REACT_193437. Polo-like kinase mediated events.
REACT_218303. Activation of ATR in response to replication stress.

Family and domain databases

Gene3Di 3.40.250.10. 1 hit.
InterProi IPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view ]
Pfami PF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view ]
PRINTSi PR00716. MPIPHPHTASE.
SMARTi SM00450. RHOD. 1 hit.
[Graphical view ]
SUPFAMi SSF52821. SSF52821. 1 hit.
PROSITEi PS50206. RHODANESE_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and sequencing of a pig cdc25 tyrosine phosphatase cDNA."
    Cui H., Xu W., Powell D., Newman B.J.
    J. Anim. Sci. 73:630-630(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Tissue: Ovary.

Entry informationi

Entry nameiMPIP3_PIG
AccessioniPrimary (citable) accession number: Q29029
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: October 29, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3