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Q29029 (MPIP3_PIG) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
M-phase inducer phosphatase 3
EC=3.1.3.48
Alternative name(s):
Dual specificity phosphatase Cdc25C
Gene names
Name:CDC25C
OrganismSus scrofa (Pig) [Reference proteome]
Taxonomic identifier9823 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length502 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. Directly dephosphorylates CDK1 and activates its kinase activity. When phosphorylated, highly effective in activating G2 cells into prophase By similarity.

Catalytic activity

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

Subunit structure

Interacts with MAPK14 and 14-3-3 proteins By similarity. When phosphorylated at Ser-122 and/or Thr-123, interacts with PLK1 By similarity.

Subcellular location

Nucleus By similarity.

Post-translational modification

Phosphorylated by CHEK1 and MAPK14 at Ser-245. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase By similarity. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-217 promotes nuclear translocation. Ser-224 is a minor phosphorylation site By similarity. Phosphorylation by CDK1 occurs at G2 and G2-M transition and leads to increased activity By similarity.

Sequence similarities

Belongs to the MPI phosphatase family.

Contains 1 rhodanese domain.

Ontologies

Keywords
   Biological processCell cycle
Cell division
Mitosis
   Cellular componentNucleus
   Molecular functionHydrolase
Protein phosphatase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell division

Inferred from electronic annotation. Source: UniProtKB-KW

mitosis

Inferred from electronic annotation. Source: UniProtKB-KW

peptidyl-tyrosine dephosphorylation

Inferred from electronic annotation. Source: GOC

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: Compara

nucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionprotein tyrosine phosphatase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 502502M-phase inducer phosphatase 3
PRO_0000198650

Regions

Domain350 – 457108Rhodanese

Sites

Active site4061 By similarity

Amino acid modifications

Modified residue301Phosphoserine By similarity
Modified residue401Phosphothreonine; by CDK1 By similarity
Modified residue501Phosphoserine By similarity
Modified residue541Phosphoserine By similarity
Modified residue571Phosphoserine By similarity
Modified residue601Phosphothreonine; by CDK1 By similarity
Modified residue1151Phosphoserine; by CDK1 By similarity
Modified residue1221Phosphoserine By similarity
Modified residue1231Phosphothreonine; by CDK1 By similarity
Modified residue1941Phosphoserine; by CDK1 By similarity
Modified residue2171Phosphoserine; by PLK3 By similarity
Modified residue2241Phosphoserine; by PLK3 By similarity
Modified residue2431Phosphoserine; by CDK1 By similarity
Modified residue2451Phosphoserine; by CHEK1, CHEK2, BRSK1 and MAPK14 By similarity
Modified residue5011Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q29029 [UniParc].

Last modified November 1, 1997. Version 1.
Checksum: 02E28CE42C0EDA70

FASTA50256,639
        10         20         30         40         50         60 
MAEGGRPGSG PSFRSNHRKI LNLLLERETS FSMSSDRPGT PLKKKLFGDS ANLSILSGGT 

        70         80         90        100        110        120 
PKRCLDLSNL SSGEMSATQF TTSADLDETG HLDSTGSEEI QLAGMSYHQH LLKCSPAQLL 

       130        140        150        160        170        180 
CSTPNALDHG RRKKDAICSS SENKENENNT SKPLEWWAHR NLLFQKRPGG PYMSPLSLLD 

       190        200        210        220        230        240 
NGNLVEGEMK HLGSPITAVS KLDKNPERGE DQAEEISDEL MEFSLEDQEE AKVSLNTSCL 

       250        260        270        280        290        300 
YRSFSLPDSL NSPGLKQVVK FKDHTLPDKV KKKYCSSHEE LRKGLGVKKM VSLCNINMIQ 

       310        320        330        340        350        360 
MLEEDSNQGP LIGDFSKVCA LPTVSGRHQD LKYVNPETVA ALLSGKFQGL IEKFYIIDCR 

       370        380        390        400        410        420 
YPYEYLGGHI QGALNLYSQE ELCNFFLKKP IIPLDTQKRI IVVFHCEFSS ERGPRMCRSL 

       430        440        450        460        470        480 
REEDRALNQY PALYYPELYI LKGGYRDFFP EYMELCEPQS YCPMHHQDHK AELLRCRSQN 

       490        500 
KAWEGERQLQ EQIALLVKDV SP

« Hide

References

[1]"Cloning and sequencing of a pig cdc25 tyrosine phosphatase cDNA."
Cui H., Xu W., Powell D., Newman B.J.
J. Anim. Sci. 73:630-630(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Ovary.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X78317 Genomic DNA. Translation: CAA55123.1.
PIRS43123.
RefSeqNP_001116567.1. NM_001123095.1.
UniGeneSsc.26311.

3D structure databases

ProteinModelPortalQ29029.
SMRQ29029. Positions 311-473.
ModBaseSearch...

Protein-protein interaction databases

STRING9823.ENSSSCP00000015243.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSSSCT00000015655; ENSSSCP00000015242; ENSSSCG00000014329.
GeneID100144474.
KEGGssc:100144474.

Organism-specific databases

CTD995.

Phylogenomic databases

eggNOGCOG5105.
GeneTreeENSGT00390000018747.
HOGENOMHOG000082672.
HOVERGENHBG052501.
KOK05867.
OMAFRSNQRK.

Family and domain databases

Gene3D3.40.250.10. 1 hit.
InterProIPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
[Graphical view]
PANTHERPTHR10828. PTHR10828. 1 hit.
PfamPF06617. M-inducer_phosp. 1 hit.
PF00581. Rhodanese. 1 hit.
[Graphical view]
PRINTSPR00716. MPIPHPHTASE.
SMARTSM00450. RHOD. 1 hit.
[Graphical view]
SUPFAMSSF52821. Rhodanese-like. 1 hit.
PROSITEPS50206. RHODANESE_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMPIP3_PIG
AccessionPrimary (citable) accession number: Q29029
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1997
Last sequence update: November 1, 1997
Last modified: April 3, 2013
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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