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Q29029

- MPIP3_PIG

UniProt

Q29029 - MPIP3_PIG

Protein

M-phase inducer phosphatase 3

Gene

CDC25C

Organism
Sus scrofa (Pig)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 88 (01 Oct 2014)
      Sequence version 1 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. Directly dephosphorylates CDK1 and activates its kinase activity. When phosphorylated, highly effective in activating G2 cells into prophase By similarity.By similarity

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei406 – 4061By similarity

    GO - Molecular functioni

    1. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. G2/M transition of mitotic cell cycle Source: Ensembl
    2. mitotic nuclear division Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    ReactomeiREACT_106629. Cyclin B2 mediated events.
    REACT_193437. Polo-like kinase mediated events.
    REACT_218303. Activation of ATR in response to replication stress.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    M-phase inducer phosphatase 3 (EC:3.1.3.48)
    Alternative name(s):
    Dual specificity phosphatase Cdc25C
    Gene namesi
    Name:CDC25C
    OrganismiSus scrofa (Pig)
    Taxonomic identifieri9823 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
    ProteomesiUP000008227: Chromosome 2

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. cytoplasm Source: Ensembl
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 502502M-phase inducer phosphatase 3PRO_0000198650Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei30 – 301PhosphoserineBy similarity
    Modified residuei40 – 401Phosphothreonine; by CDK1By similarity
    Modified residuei50 – 501PhosphoserineBy similarity
    Modified residuei54 – 541PhosphoserineBy similarity
    Modified residuei57 – 571PhosphoserineBy similarity
    Modified residuei60 – 601Phosphothreonine; by CDK1By similarity
    Modified residuei115 – 1151Phosphoserine; by CDK1By similarity
    Modified residuei122 – 1221PhosphoserineBy similarity
    Modified residuei123 – 1231Phosphothreonine; by CDK1By similarity
    Modified residuei194 – 1941Phosphoserine; by CDK1By similarity
    Modified residuei217 – 2171Phosphoserine; by PLK3By similarity
    Modified residuei224 – 2241Phosphoserine; by PLK3By similarity
    Modified residuei243 – 2431Phosphoserine; by CDK1By similarity
    Modified residuei245 – 2451Phosphoserine; by CHEK1, CHEK2, BRSK1 and MAPK14By similarity
    Modified residuei501 – 5011PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylated by CHEK1 and MAPK14 at Ser-245. This phosphorylation creates a binding site for 14-3-3 protein and inhibits the phosphatase By similarity. Phosphorylated by PLK4. Phosphorylated by PLK1, leading to activate the phosphatase activity. Phosphorylation by PLK3 at Ser-217 promotes nuclear translocation. Ser-224 is a minor phosphorylation site By similarity. Phosphorylation by CDK1 occurs at G2 and G2-M transition and leads to increased activity By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Subunit structurei

    Interacts with MAPK14 and 14-3-3 proteins. When phosphorylated at Ser-122 and/or Thr-123, interacts with PLK1.By similarity

    Protein-protein interaction databases

    STRINGi9823.ENSSSCP00000015243.

    Structurei

    3D structure databases

    ProteinModelPortaliQ29029.
    SMRiQ29029. Positions 311-473.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini350 – 457108RhodanesePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the MPI phosphatase family.Curated
    Contains 1 rhodanese domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5105.
    GeneTreeiENSGT00390000018747.
    HOGENOMiHOG000082672.
    HOVERGENiHBG052501.
    KOiK05867.
    OMAiTVSLCDI.
    OrthoDBiEOG7288R1.
    TreeFamiTF101056.

    Family and domain databases

    Gene3Di3.40.250.10. 1 hit.
    InterProiIPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view]
    PfamiPF06617. M-inducer_phosp. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view]
    PRINTSiPR00716. MPIPHPHTASE.
    SMARTiSM00450. RHOD. 1 hit.
    [Graphical view]
    SUPFAMiSSF52821. SSF52821. 1 hit.
    PROSITEiPS50206. RHODANESE_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q29029-1 [UniParc]FASTAAdd to Basket

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    MAEGGRPGSG PSFRSNHRKI LNLLLERETS FSMSSDRPGT PLKKKLFGDS    50
    ANLSILSGGT PKRCLDLSNL SSGEMSATQF TTSADLDETG HLDSTGSEEI 100
    QLAGMSYHQH LLKCSPAQLL CSTPNALDHG RRKKDAICSS SENKENENNT 150
    SKPLEWWAHR NLLFQKRPGG PYMSPLSLLD NGNLVEGEMK HLGSPITAVS 200
    KLDKNPERGE DQAEEISDEL MEFSLEDQEE AKVSLNTSCL YRSFSLPDSL 250
    NSPGLKQVVK FKDHTLPDKV KKKYCSSHEE LRKGLGVKKM VSLCNINMIQ 300
    MLEEDSNQGP LIGDFSKVCA LPTVSGRHQD LKYVNPETVA ALLSGKFQGL 350
    IEKFYIIDCR YPYEYLGGHI QGALNLYSQE ELCNFFLKKP IIPLDTQKRI 400
    IVVFHCEFSS ERGPRMCRSL REEDRALNQY PALYYPELYI LKGGYRDFFP 450
    EYMELCEPQS YCPMHHQDHK AELLRCRSQN KAWEGERQLQ EQIALLVKDV 500
    SP 502
    Length:502
    Mass (Da):56,639
    Last modified:November 1, 1997 - v1
    Checksum:i02E28CE42C0EDA70
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78317 Genomic DNA. Translation: CAA55123.1.
    PIRiS43123.
    RefSeqiNP_001116567.1. NM_001123095.1.
    XP_005661723.1. XM_005661666.1.
    XP_005661724.1. XM_005661667.1.
    XP_005661725.1. XM_005661668.1.
    XP_005661726.1. XM_005661669.1.
    UniGeneiSsc.26311.

    Genome annotation databases

    EnsembliENSSSCT00000015655; ENSSSCP00000015242; ENSSSCG00000014329.
    GeneIDi100144474.
    KEGGissc:100144474.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78317 Genomic DNA. Translation: CAA55123.1 .
    PIRi S43123.
    RefSeqi NP_001116567.1. NM_001123095.1.
    XP_005661723.1. XM_005661666.1.
    XP_005661724.1. XM_005661667.1.
    XP_005661725.1. XM_005661668.1.
    XP_005661726.1. XM_005661669.1.
    UniGenei Ssc.26311.

    3D structure databases

    ProteinModelPortali Q29029.
    SMRi Q29029. Positions 311-473.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9823.ENSSSCP00000015243.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSSSCT00000015655 ; ENSSSCP00000015242 ; ENSSSCG00000014329 .
    GeneIDi 100144474.
    KEGGi ssc:100144474.

    Organism-specific databases

    CTDi 995.

    Phylogenomic databases

    eggNOGi COG5105.
    GeneTreei ENSGT00390000018747.
    HOGENOMi HOG000082672.
    HOVERGENi HBG052501.
    KOi K05867.
    OMAi TVSLCDI.
    OrthoDBi EOG7288R1.
    TreeFami TF101056.

    Enzyme and pathway databases

    Reactomei REACT_106629. Cyclin B2 mediated events.
    REACT_193437. Polo-like kinase mediated events.
    REACT_218303. Activation of ATR in response to replication stress.

    Family and domain databases

    Gene3Di 3.40.250.10. 1 hit.
    InterProi IPR000751. MPI_Phosphatase.
    IPR001763. Rhodanese-like_dom.
    [Graphical view ]
    Pfami PF06617. M-inducer_phosp. 1 hit.
    PF00581. Rhodanese. 1 hit.
    [Graphical view ]
    PRINTSi PR00716. MPIPHPHTASE.
    SMARTi SM00450. RHOD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52821. SSF52821. 1 hit.
    PROSITEi PS50206. RHODANESE_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of a pig cdc25 tyrosine phosphatase cDNA."
      Cui H., Xu W., Powell D., Newman B.J.
      J. Anim. Sci. 73:630-630(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Tissue: Ovary.

    Entry informationi

    Entry nameiMPIP3_PIG
    AccessioniPrimary (citable) accession number: Q29029
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1997
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 88 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3