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Protein

Histone-lysine N-methyltransferase eggless

Gene

egg

Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 in ovary. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting Su(var)205/HP1 to methylated histones. Plays a central role during oogenesis (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi941Zinc 1By similarity1
Metal bindingi941Zinc 2By similarity1
Metal bindingi943Zinc 1By similarity1
Metal bindingi947Zinc 1By similarity1
Metal bindingi947Zinc 3By similarity1
Metal bindingi953Zinc 1By similarity1
Metal bindingi955Zinc 2By similarity1
Metal bindingi993Zinc 2By similarity1
Metal bindingi993Zinc 3By similarity1
Metal bindingi997Zinc 2By similarity1
Metal bindingi999Zinc 3By similarity1
Metal bindingi1003Zinc 3By similarity1
Binding sitei1062S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei1064S-adenosyl-L-methioninePROSITE-ProRule annotation1
Binding sitei1183S-adenosyl-L-methioninePROSITE-ProRule annotation1
Metal bindingi1189Zinc 4By similarity1
Metal bindingi1242Zinc 4By similarity1
Metal bindingi1244Zinc 4By similarity1
Metal bindingi1249Zinc 4By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Developmental protein, Methyltransferase, Repressor, Transferase

Keywords - Biological processi

Differentiation, Oogenesis, Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, S-adenosyl-L-methionine, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase eggless (EC:2.1.1.43)
Alternative name(s):
SETDB1 homolog
Gene namesi
Name:egg
ORF Names:GA30484
OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
Taxonomic identifieri46245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000001819 Componentsi: Chromosome 3, Partially assembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0265021. Dpse\GA30484.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002818231 – 1254Histone-lysine N-methyltransferase egglessAdd BLAST1254

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini535 – 607Tudor 1Add BLAST73
Domaini634 – 691Tudor 2Add BLAST58
Domaini811 – 877MBDPROSITE-ProRule annotationAdd BLAST67
Domaini939 – 1011Pre-SETPROSITE-ProRule annotationAdd BLAST73
Domaini1014 – 1229SETPROSITE-ProRule annotationAdd BLAST216
Domaini1238 – 1254Post-SETPROSITE-ProRule annotationAdd BLAST17

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1024 – 1026S-adenosyl-L-methionine bindingBy similarity3
Regioni1186 – 1187S-adenosyl-L-methionine bindingBy similarity2

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili391 – 416Sequence analysisAdd BLAST26

Domaini

In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.By similarity

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.PROSITE-ProRule annotation
Contains 1 MBD (methyl-CpG-binding) domain.PROSITE-ProRule annotation
Contains 1 post-SET domain.PROSITE-ProRule annotation
Contains 1 pre-SET domain.PROSITE-ProRule annotation
Contains 1 SET domain.PROSITE-ProRule annotation
Contains 2 Tudor domains.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

InParanoidiQ28Z18.
KOiK11421.
OMAiIAFFDGT.

Family and domain databases

Gene3Di3.30.890.10. 1 hit.
InterProiIPR016177. DNA-bd_dom.
IPR001739. Methyl_CpG_DNA-bd.
IPR003616. Post-SET_dom.
IPR007728. Pre-SET_dom.
IPR001214. SET_dom.
[Graphical view]
PfamiPF01429. MBD. 1 hit.
PF05033. Pre-SET. 1 hit.
PF00856. SET. 1 hit.
[Graphical view]
SMARTiSM00391. MBD. 1 hit.
SM00468. PreSET. 1 hit.
SM00317. SET. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS50982. MBD. 1 hit.
PS50868. POST_SET. 1 hit.
PS50867. PRE_SET. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q28Z18-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASESAAMDI LDSAEASLNV ASTALVEKEK KVQPATDETT PEKKAKMEID
60 70 80 90 100
AEMKDLTNDI PNSRKSQEKD PDALEDAKDP EADSSIELLC SPTPAEATDV
110 120 130 140 150
DAMASKTESD NSVELLESPL KSPSSNDVND EELLPLEEKE KPGPAKELEP
160 170 180 190 200
KESEPDSKES SKSEALADSS IELISSPTSD DSLAKEKEVE VKEEHGQQAE
210 220 230 240 250
AQVLQEIPRK ADDSFKLQDD IAMEEDVPVP RSKAMQESKE TQKTSKTTTK
260 270 280 290 300
LEPLVDSIKD AIEDCINCNC KRLKKQYVLA CVAILNFYKV PRKLKRSQYV
310 320 330 340 350
CLDCYDTAVE MYEEYAGLLL AKQPLLLREF KQEQADFVTL DSSDEEEDEK
360 370 380 390 400
TPEKPEFSKN VLDLIENELE DAIKKTLNKV EFSNQFNWSK TILQAKIERL
410 420 430 440 450
AKKFEEVDLQ LAQVQGLADK MHCSVYNSCQ VVHKQLPPLD LHQNICPSDY
460 470 480 490 500
KRLQQLPAAG DIVRPPIKIG ETYYAVKNKA IASWVSVSVM EICDTTTGGG
510 520 530 540 550
VTVKAYKIKY QHMPYPMMKT VAAKHLAYFD PPTVRLPIGT RVIAFFDGTL
560 570 580 590 600
VGGKEKGVVQ SAFYPGIIAE PLKQNNRFRY LIFYDDGYTQ YVHHSDVRLV
610 620 630 640 650
CQASEKVWED VHPASRDFIQ KYVERYAVDR PMVQCTKGQS MNTESNGTWL
660 670 680 690 700
YARVIEVDCS LVLMQFEADK NHTEWIYRGS LRLAPVFKET QNSLNADCAI
710 720 730 740 750
HQMRVPRRTE PFIRYTKEME SSNMQVDQQI RAIARKSTSK SGSPASTAAP
760 770 780 790 800
PTGSSSSSAV RHLNNSTIYV DDDTRPKGQV VHFTAKRNMP PKIFKSHKCN
810 820 830 840 850
PGCPFPMMHR LDSYSPLSKP LLSGWERMFM KQKTKRTVVY RGPCGRNLRN
860 870 880 890 900
MAEVHNYLRL TNNVLNVDNF DFTPDLRCLA EYYIESTIVK EADISKGQEK
910 920 930 940 950
MAIPLVNYYD NTLPPPCEYA KQRIPTEGVN LNLDEEFLVC CDCEDDCSDK
960 970 980 990 1000
ESCACWQLTV TGVRYCNPKK PIEEIGYQYK RLHEGVLTGI YECNSRCKCK
1010 1020 1030 1040 1050
KNCLNRVVQH SLEMKLQVFK TSNRGWGLRC VNDIPKGAFV CIYAGHLLTE
1060 1070 1080 1090 1100
AKANEGGQDA GDEYFADLDY IEVAEQLKEG YESDVERADL DHEDDNYGPD
1110 1120 1130 1140 1150
AEDDDDFRPN NYYQKKKEKL RSSRSNSSST QNTELDSQER TVISFNPNTD
1160 1170 1180 1190 1200
LDETVRENSV RRFFGKDQTP FIMDAKTTGN LGRYFNHSCS PNLFVQNVFV
1210 1220 1230 1240 1250
DTHDLRFPWV GFFASSHIRS GTELTWNYNY EVGVVPNKVL YCQCGAQNCR

VRLL
Length:1,254
Mass (Da):142,107
Last modified:May 14, 2014 - v2
Checksum:iABAC6B755D26C097
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000071 Genomic DNA. Translation: EDY69183.2.
RefSeqiXP_002138625.3. XM_002138589.3.

Genome annotation databases

EnsemblMetazoaiFBtr0337134; FBpp0308048; FBgn0265021.
GeneIDi6898620.
KEGGidpo:Dpse_GA30484.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CM000071 Genomic DNA. Translation: EDY69183.2.
RefSeqiXP_002138625.3. XM_002138589.3.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0337134; FBpp0308048; FBgn0265021.
GeneIDi6898620.
KEGGidpo:Dpse_GA30484.

Organism-specific databases

FlyBaseiFBgn0265021. Dpse\GA30484.

Phylogenomic databases

InParanoidiQ28Z18.
KOiK11421.
OMAiIAFFDGT.

Family and domain databases

Gene3Di3.30.890.10. 1 hit.
InterProiIPR016177. DNA-bd_dom.
IPR001739. Methyl_CpG_DNA-bd.
IPR003616. Post-SET_dom.
IPR007728. Pre-SET_dom.
IPR001214. SET_dom.
[Graphical view]
PfamiPF01429. MBD. 1 hit.
PF05033. Pre-SET. 1 hit.
PF00856. SET. 1 hit.
[Graphical view]
SMARTiSM00391. MBD. 1 hit.
SM00468. PreSET. 1 hit.
SM00317. SET. 1 hit.
[Graphical view]
SUPFAMiSSF54171. SSF54171. 1 hit.
PROSITEiPS50982. MBD. 1 hit.
PS50868. POST_SET. 1 hit.
PS50867. PRE_SET. 1 hit.
PS50280. SET. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSETB1_DROPS
AccessioniPrimary (citable) accession number: Q28Z18
Secondary accession number(s): B5DZD5, B5DZD6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 3, 2007
Last sequence update: May 14, 2014
Last modified: May 11, 2016
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.