Q28Z18 (SETB1_DROPS) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone-lysine N-methyltransferase eggless
EC=2.1.1.43

Alternative name(s):
SETDB1 homolog

Gene names

Name:	egg
ORF Names:	GA24879

Organism

Drosophila pseudoobscura pseudoobscura (Fruit fly) [Reference proteome]

Taxonomic identifier

46245 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › obscura group › pseudoobscura subgroup › Drosophila pseudoobscura

Protein attributes

Sequence length	1314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 in ovary. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting Su(var)205/HP1 to methylated histones. Plays a central role during oogenesis By similarity.
Catalytic activity	S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
Subcellular location	Nucleus By similarity. Chromosome By similarity.
Sequence similarities	Belongs to the histone-lysine methyltransferase family. Suvar3-9 subfamily. Contains 1 MBD (methyl-CpG-binding) domain. Contains 1 post-SET domain. Contains 1 pre-SET domain. Contains 1 SET domain. Contains 2 Tudor domains.
Sequence caution	The sequence EDY69182.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence EDY69183.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Differentiation Oogenesis Transcription Transcription regulation
Cellular component	Chromosome Nucleus
Domain	Coiled coil Repeat
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Developmental protein Methyltransferase Repressor Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of transcription during meiosis Inferred from sequence or structural similarity. Source: UniProtKB oogenesis Inferred from sequence or structural similarity. Source: UniProtKB transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chromosome Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro histone-lysine N-methyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1314	1314	Histone-lysine N-methyltransferase eggless		PRO_0000281823
Regions
	Domain	595 – 667	73	Tudor 1
	Domain	694 – 751	58	Tudor 2
	Domain	871 – 937	67	MBD
	Domain	999 – 1071	73	Pre-SET
	Domain	1073 – 1293	221	SET
	Domain	1298 – 1314	17	Post-SET
	Coiled coil	451 – 476	26	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q28Z18 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 734E68538BB197CF	FASTA	1,314	148,837
	10 20 30 40 50 60 MASESAAMDI LDSAEASLNV ASTALVEKEK KVQPATDETT PEKKAKMEID AEMKDLTNDI 70 80 90 100 110 120 PNSRKSQEKD PDALEDAKDP EADSSIELLC SPTPAEATDV DAMASKTESD NSVELLESPL 130 140 150 160 170 180 KSPSSNDVND EELLPLEEKE KPGPAKELEP KESEPDSKES SKSEALADSS IELISSPTSD 190 200 210 220 230 240 DSLAKEKEVE VKEEHGQQAE AQVLQEIPRK ADDSFKLQDD IAMEEDVPVP RSKAMQESKE 250 260 270 280 290 300 TQKTSKTTTK LEPLVDSIKD AIEGEPKDKR NNNVDSIESH IESAKKSDNE ILAVDLEIGS 310 320 330 340 350 360 PPKEAEEKSK TDFSWDGRIS YNKDCINCNC KRLKKQYVLA CVAILNFYKV PRKLKRSQYV 370 380 390 400 410 420 CLDCYDTAVE MYEEYAGLLL AKQPLLLREF KQEQADFVTL DSSDEEEDEK TPEKPEFSKN 430 440 450 460 470 480 VLDLIENELE DAIKKTLNKV EFSNQFNWSK TILQAKIERL AKKFEEVDLQ LAQVQGLADK 490 500 510 520 530 540 MHCSVYNSCQ VVHKQLPPLD LHQNICPSDY KRLQQLPAAG DIVRPPIKIG ETYYAVKNKA 550 560 570 580 590 600 IASWVSVSVM EICDTTTGGG VTVKAYKIKY QHMPYPMMKT VAAKHLAYFD PPTVRLPIGT 610 620 630 640 650 660 RVIAFFDGTL VGGKEKGVVQ SAFYPGIIAE PLKQNNRFRY LIFYDDGYTQ YVHHSDVRLV 670 680 690 700 710 720 CQASEKVWED VHPASRDFIQ KYVERYAVDR PMVQCTKGQS MNTESNGTWL YARVIEVDCS 730 740 750 760 770 780 LVLMQFEADK NHTEWIYRGS LRLAPVFKET QNSLNADCAI HQMRVPRRTE PFIRYTKEME 790 800 810 820 830 840 SSNMQVDQQI RAIARKSTSK SGSPASTAAP PTGSSSSSAV RHLNNSTIYV DDDTRPKGQV 850 860 870 880 890 900 VHFTAKRNMP PKIFKSHKCN PGCPFPMMHR LDSYSPLSKP LLSGWERMFM KQKTKRTVVY 910 920 930 940 950 960 RGPCGRNLRN MAEVHNYLRL TNNVLNVDNF DFTPDLRCLA EYYIESTIVK EADISKGQEK 970 980 990 1000 1010 1020 MAIPLVNYYD NTLPPPCEYA KQRIPTEGVN LNLDEEFLVC CDCEDDCSDK ESCACWQLTV 1030 1040 1050 1060 1070 1080 TGVRYCNPKK PIEEIGYQYK RLHEGVLTGI YECNSRCKCK KNCLNRVVQH SLEMKLQVFK 1090 1100 1110 1120 1130 1140 TSNRGWGLRC VNDIPKGAFV CIYAGHLLTE AKANEGGQDA GDEYFADLDY IEVAEQLKEG 1150 1160 1170 1180 1190 1200 YESDVERADL DHEDDNYGPD AEDDDDFRPN NYYQKKKEKL RSSRSNSSST QNTELDSQER 1210 1220 1230 1240 1250 1260 TVISFNPNTD LDETVRENSV RRFFGKDQTP FIMDAKTTGN LGRYFNHSCS PNLFVQNVFV 1270 1280 1290 1300 1310 DTHDLRFPWV GFFASSHIRS GTELTWNYNY EVGVVPNKVL YCQCGAQNCR VRLL

References

[1]

"Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution." Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L. , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A. Genome Res. 15:1-18(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MV2-25 / Tucson 14011-0121.94.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CM000071 Genomic DNA. Translation: EDY69182.1. Sequence problems. CM000071 Genomic DNA. Translation: EDY69183.1. Sequence problems.
RefSeq	XP_002138624.1. XM_002138588.1. XP_002138625.1. XM_002138589.1.
3D structure databases
ProteinModelPortal	Q28Z18.
ModBase	Search...
Protein-protein interaction databases
STRING	7237.FBpp0278125.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	6898619. 6898620.
KEGG	dpo:Dpse_GA24879. dpo:Dpse_GA25603.
Organism-specific databases
FlyBase	FBgn0246264. Dpse\GA24879.
Phylogenomic databases
eggNOG	COG2940.
InParanoid	Q28Z18.
KO	K11421.
OMA	CKCKKNC.
OrthoDB	EOG4THT82.
Family and domain databases
Gene3D	3.30.890.10. 1 hit.
InterPro	IPR016177. DNA-bd_integrase-typ. IPR001739. Methyl_CpG_DNA-bd. IPR003616. Post-SET_dom. IPR007728. Pre-SET_dom. IPR001214. SET_dom. [Graphical view]
Pfam	PF01429. MBD. 1 hit. PF05033. Pre-SET. 1 hit. PF00856. SET. 1 hit. [Graphical view]
SMART	SM00317. SET. 1 hit. [Graphical view]
SUPFAM	SSF54171. DNA-binding_integrase-type. 1 hit.
PROSITE	PS50982. MBD. 1 hit. PS50868. POST_SET. 1 hit. PS50867. PRE_SET. 1 hit. PS50280. SET. 1 hit. PS50304. TUDOR. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SETB1_DROPS

Accession

Primary (citable) accession number: Q28Z18
Secondary accession number(s): B5DZD5, B5DZD6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 3, 2007
Last sequence update:	April 4, 2006
Last modified:	May 1, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project