ID   HOT_DROPS               Reviewed;         464 AA.
AC   Q28XT3;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-JAN-2009, sequence version 2.
DT   16-JUN-2009, entry version 23.
DE   RecName: Full=Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial;
DE            Short=HOT;
DE            EC=1.1.99.24;
DE   Flags: Precursor;
GN   ORFNames=GA17444;
OS   Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC   Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha;
OC   Ephydroidea; Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=46245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MV2-25;
RX   PubMed=15632085; DOI=10.1101/gr.3059305;
RA   Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA   Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P.,
RA   Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J.,
RA   van Batenburg M.F., Howells S.L., Scherer S.E., Sodergren E.,
RA   Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D.,
RA   Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D.,
RA   Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R.,
RA   Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L.,
RA   Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA   Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA   Weinstock G.M., Gibbs R.A.;
RT   "Comparative genome sequencing of Drosophila pseudoobscura:
RT   chromosomal, gene, and cis-element evolution.";
RL   Genome Res. 15:1-18(2005).
CC   -!- FUNCTION: Catalyzes the cofactor-independent reversible oxidation
CC       of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA)
CC       coupled to reduction of 2-ketoglutarate (2-KG) to D-2-
CC       hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a
CC       substrate for HOT when using 2-KG as hydrogen acceptor, resulting
CC       in the formation of D-2-HG (By similarity).
CC   -!- CATALYTIC ACTIVITY: (S)-3-hydroxybutanoate + 2-oxoglutarate =
CC       acetoacetate + (R)-2-hydroxyglutarate.
CC   -!- CATALYTIC ACTIVITY: 2-oxoglutaric acid + 4-hydroxybutanoic acid =
CC       (R)-2-hydroxyglutaric acid + succinic semialdehyde.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion (By similarity).
CC   -!- SIMILARITY: Belongs to the iron-containing alcohol dehydrogenase
CC       family. Hydroxyacid-oxoacid transhydrogenase subfamily.
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DR   EMBL; CM000071; EAL26233.2; -; Genomic_DNA.
DR   RefSeq; XP_001361654.2; -.
DR   GeneID; 4805213; -.
DR   KEGG; dpo:Dpse_GA17444; -.
DR   FlyBase; FBgn0077457; Dpse\GA17444.
DR   BRENDA; 1.1.99.24; 190795.
DR   GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR   GO; GO:0047988; F:hydroxyacid-oxoacid transhydrogenase activity; ISS:UniProtKB.
DR   GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR   GO; GO:0015993; P:molecular hydrogen transport; ISS:UniProtKB.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001670; ADH_Fe.
DR   InterPro; IPR018211; ADH_Fe_CS.
DR   Pfam; PF00465; Fe-ADH; 1.
DR   PROSITE; PS00913; ADH_IRON_1; FALSE_NEG.
DR   PROSITE; PS00060; ADH_IRON_2; FALSE_NEG.
PE   3: Inferred from homology;
KW   Complete proteome; Mitochondrion; Oxidoreductase; Transit peptide.
FT   TRANSIT       1      ?       Mitochondrion (Potential).
FT   CHAIN         ?    464       Probable hydroxyacid-oxoacid
FT                                transhydrogenase, mitochondrial.
FT                                /FTId=PRO_0000323006.
SQ   SEQUENCE   464 AA;  50033 MW;  4769F346C24C90B1 CRC64;
     MSRKNVLNLI NTIVANSCKC PAHSHNYGSA APTGVQTGQK EYAFEMSAST VRFGPGVSVE
     VGADLRNLGA KKVCLVTDRN VAKLPSVKVA LDSLARHGIN YEVYDETRVE PTDASLWHAA
     DYARQNEFDA FLAIGGGSVM DTAKAANLFS SDREAEFLDY VNCPIGKGKE ISVKLKPLIA
     MPTTSGTGSE TTGVAIFDYK RLHAKTGISS KYLKPTLAII DPLHTLSQPE RVMAFAGFDV
     FCHALESFTA VDYRERGPAP SDPSLRPTYQ GRNPISDVWA RFALETIRKN FINAIYEPGN
     LEARSQMHLA STMAGVGFGN AGVHLCHGLS YPISGNVRNY KPAGYTADHA LIPHGLSVVI
     SAPAVFEFTA PACPDRHLEA AKLLGAKVDG VRASDAGRLL ADTVRGFMQR AGIENGLREL
     GFSSSDVPNL VDGTLPQERI TKLAPRAQTQ ENLAQLFENS MVAY
//