Reviewed, UniProtKB/Swiss-Prot Q28XT3 (HOT_DROPS)

Names and origin

Protein names

Recommended name:
    Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial
      Short name=HOT
    EC=1.1.99.24

Gene names

ORF Names:

GA17444

Organism

Drosophila pseudoobscura pseudoobscura (Fruit fly) [Complete proteome]

Taxonomic identifier

46245 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG By similarity.
Catalytic activity	(S)-3-hydroxybutanoate + 2-oxoglutarate = acetoacetate + (R)-2-hydroxyglutarate. 2-oxoglutaric acid + 4-hydroxybutanoic acid = (R)-2-hydroxyglutaric acid + succinic semialdehyde.
Subcellular location	Mitochondrion By similarity.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	molecular hydrogen transport Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	hydroxyacid-oxoacid transhydrogenase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 464		Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial		PRO_0000323006

Sequences

	Sequence		Length	Mass (Da)
	Q28XT3-1 [UniParc]. Last modified January 20, 2009. Version 2. Checksum: 4769F346C24C90B1	FASTA	464	50,033
	10 20 30 40 50 60 MSRKNVLNLI NTIVANSCKC PAHSHNYGSA APTGVQTGQK EYAFEMSAST VRFGPGVSVE 70 80 90 100 110 120 VGADLRNLGA KKVCLVTDRN VAKLPSVKVA LDSLARHGIN YEVYDETRVE PTDASLWHAA 130 140 150 160 170 180 DYARQNEFDA FLAIGGGSVM DTAKAANLFS SDREAEFLDY VNCPIGKGKE ISVKLKPLIA 190 200 210 220 230 240 MPTTSGTGSE TTGVAIFDYK RLHAKTGISS KYLKPTLAII DPLHTLSQPE RVMAFAGFDV 250 260 270 280 290 300 FCHALESFTA VDYRERGPAP SDPSLRPTYQ GRNPISDVWA RFALETIRKN FINAIYEPGN 310 320 330 340 350 360 LEARSQMHLA STMAGVGFGN AGVHLCHGLS YPISGNVRNY KPAGYTADHA LIPHGLSVVI 370 380 390 400 410 420 SAPAVFEFTA PACPDRHLEA AKLLGAKVDG VRASDAGRLL ADTVRGFMQR AGIENGLREL 430 440 450 460 GFSSSDVPNL VDGTLPQERI TKLAPRAQTQ ENLAQLFENS MVAY

References

[1]

"Comparative genome sequencing of Drosophila pseudoobscura: chromosomal, gene, and cis-element evolution." Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S., Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P., Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J., van Batenburg M.F., Howells S.L. , Scherer S.E., Sodergren E., Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D., Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D., Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R., Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L., Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F., Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M., Weinstock G.M., Gibbs R.A. Genome Res. 15:1-18(2005) [PubMed: 15632085] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: MV2-25.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CM000071 Genomic DNA. Translation: EAL26233.2.
RefSeq	XP_001361654.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	4805213.
KEGG	dpo:Dpse_GA17444.
Organism-specific databases
FlyBase	FBgn0077457. Dpse\GA17444.
Enzyme and pathway databases
BRENDA	1.1.99.24. 190795.
Family and domain databases
InterPro	IPR001670. ADH_Fe. IPR018211. ADH_Fe_CS. [Graphical view]
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. False negative. PS00060. ADH_IRON_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOT_DROPS

Accession

Primary (citable) accession number: Q28XT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	January 20, 2009
Last modified:	June 16, 2009
This is version 23 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project