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Q28WG2 (DAPA_JANSC) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Dihydrodipicolinate synthase
Short name=DHDPS
EC=4.2.1.52
Gene names
Name:dapA
Ordered Locus Names:Jann_0033
OrganismJannaschia sp. (strain CCS1) [Complete proteome] [HAMAP]
Taxonomic identifier290400 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Jannaschia

Protein attributes

Sequence length290 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418

Subunit structure

Homotetramer By similarity. HAMAP MF_00418

Subcellular location

Cytoplasm By similarity HAMAP MF_00418.

Sequence similarities

Belongs to the DHDPS family.

Sequence caution

The sequence ABD52950.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Diaminopimelate biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   LigandSchiff base
   Molecular functionLyase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processdiaminopimelate biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiondihydrodipicolinate synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 290290Dihydrodipicolinate synthase HAMAP MF_00418
PRO_0000340958

Regions

Region47 – 482Pyruvate binding By similarity

Sites

Active site1591Schiff-base intermediate with substrate By similarity
Binding site1051Pyruvate By similarity
Site1311Involved in proton transfer during cleavage By similarity

Sequences

Sequence LengthMass (Da)Tools
Q28WG2 [UniParc].

Last modified June 10, 2008. Version 2.
Checksum: 1C70E11F08020D89

FASTA29030,629
        10         20         30         40         50         60 
MFQGSMPALV TPMTNGAVDF EVLKRLVDWH VEEGSNGLVP VGTTGESPTL THEEHEQVIE 

        70         80         90        100        110        120 
TVVKAAGGRV PVIAGAGSNN TAEAMRHVQH AKDVGADATL VVTPYYNKPT QRGLIAHYEA 

       130        140        150        160        170        180 
LAEVGIPIFI YNIPGRSVVD MTPETMGELA KHPMIIGVKD ATADMARVSR QRETCGTDFV 

       190        200        210        220        230        240 
QMSAEDASAL GFNAHGGVGC ISVTANVAPK LCAEFQAAMG AGDYTEALAY QDRLMPLHNA 

       250        260        270        280        290 
IFTEPGLCGV KYAMSVLGLC SDEVRLPLVG VDESTKAAIE AALRHAGLLS

« Hide

References

[1]"Complete sequence of chromosome of Jannaschia sp. CCS1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Chertkov O., Saunders E., Schmutz J., Larimer F. expand/collapse author list , Land M., Kyrpides N., Lykidis A., Moran M.A., Belas R., Ye W., Buchan A., Gonzalez J.M., Schell M.A., Richardson P.
Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CCS1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP000264 Genomic DNA. Translation: ABD52950.1. Different initiation.
RefSeqYP_507975.1. NC_007802.1.

3D structure databases

ProteinModelPortalQ28WG2.
SMRQ28WG2. Positions 1-289.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ28WG2.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID3932469.
GenomeReviewsGene locus Jann_0033 in contig CP000264_GR.
KEGGjan:Jann_0033.
PATRIC22143656. VBIJanSp43325_0107.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0329.
HOGENOMHBG358848.
OMANITATPY.
ProtClustDBPRK03170.

Enzyme and pathway databases

BioCycJSP290400:JANN_0033-MONOMER.

Family and domain databases

HAMAPMF_00418. DapA.
[Tree]
InterProIPR013785. Aldolase_TIM.
IPR002220. Dihydrodipicolinate_synth-like.
IPR020625. Dihydrodipicolinate_synth_AS.
IPR020624. Dihydrodipicolinate_synth_CS.
IPR005263. Dihydrodipicolinate_synth_DapA.
[Graphical view]
Gene3DG3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KOK01714.
PANTHERPTHR12128. DHDPS. 1 hit.
PfamPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFPIRSF001365. DHDPS. 1 hit.
PRINTSPR00146. DHPICSNTHASE.
TIGRFAMsTIGR00674. DapA. 1 hit.
PROSITEPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPA_JANSC
AccessionPrimary (citable) accession number: Q28WG2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: June 10, 2008
Last modified: January 25, 2012
This is version 44 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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