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Protein

Shikimate dehydrogenase (NADP(+))

Gene

aroE

Organism
Jannaschia sp. (strain CCS1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).UniRule annotation

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.UniRule annotation

Pathwayi: chorismate biosynthesis

This protein is involved in step 4 of the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate.UniRule annotation
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. 3-dehydroquinate synthase (aroB)
  3. 3-dehydroquinate dehydratase (aroQ)
  4. Shikimate dehydrogenase (NADP(+)) (aroE)
  5. Shikimate kinase (aroK), Shikimate kinase (aroK)
  6. 3-phosphoshikimate 1-carboxyvinyltransferase (aroA)
  7. Chorismate synthase (aroC)
This subpathway is part of the pathway chorismate biosynthesis, which is itself part of Metabolic intermediate biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate, the pathway chorismate biosynthesis and in Metabolic intermediate biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei66 – 661ShikimateUniRule annotation
Active sitei70 – 701Proton acceptorUniRule annotation
Binding sitei82 – 821NADPUniRule annotation
Binding sitei91 – 911ShikimateUniRule annotation
Binding sitei107 – 1071ShikimateUniRule annotation
Binding sitei222 – 2221NADP; via carbonyl oxygenUniRule annotation
Binding sitei224 – 2241ShikimateUniRule annotation
Binding sitei245 – 2451NADP; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi133 – 1375NADPUniRule annotation
Nucleotide bindingi157 – 1626NADPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciJSP290400:GI1R-197-MONOMER.
UniPathwayiUPA00053; UER00087.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate dehydrogenase (NADP(+))UniRule annotation (EC:1.1.1.25UniRule annotation)
Short name:
SDHUniRule annotation
Gene namesi
Name:aroEUniRule annotation
Ordered Locus Names:Jann_0196
OrganismiJannaschia sp. (strain CCS1)
Taxonomic identifieri290400 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeJannaschia
Proteomesi
  • UP000008326 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 278278Shikimate dehydrogenase (NADP(+))PRO_0000325126Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi290400.Jann_0196.

Structurei

3D structure databases

ProteinModelPortaliQ28VZ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni19 – 213Shikimate bindingUniRule annotation

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
HOGENOMiHOG000237875.
KOiK00014.
OMAiKVAIMEH.
OrthoDBiEOG64R67G.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q28VZ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTRDTIPLAG VIGDPISHSL SPRLHGHWLR RYGLQGHYVP LHVNHANLET
60 70 80 90 100
VLRTLPLMGF VGVNVTLPHK EHVLSIADSV TDRAALIGAA NTLTFTANEQ
110 120 130 140 150
IQADNTDGMG FLSNIRQALP GWSASAGPAL VLGSGGAAKA IVSALVSDGA
160 170 180 190 200
PVVHVANRTR ARADGLKEQF GARVSPSDWT HIPDLIGDAA LIVNTTSLGM
210 220 230 240 250
AGQSPLSLDL SRLSPPTVVT DIVYAPLQTN LLRDASIRGC ETVDGLGMLL
260 270
HQAVPGFERW FNYTPTVDED LREAVLAG
Length:278
Mass (Da):29,474
Last modified:April 4, 2006 - v1
Checksum:iCBD6EC43F8E03388
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000264 Genomic DNA. Translation: ABD53113.1.
RefSeqiWP_011453322.1. NC_007802.1.

Genome annotation databases

EnsemblBacteriaiABD53113; ABD53113; Jann_0196.
KEGGijan:Jann_0196.
PATRICi22143988. VBIJanSp43325_0272.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000264 Genomic DNA. Translation: ABD53113.1.
RefSeqiWP_011453322.1. NC_007802.1.

3D structure databases

ProteinModelPortaliQ28VZ9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290400.Jann_0196.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD53113; ABD53113; Jann_0196.
KEGGijan:Jann_0196.
PATRICi22143988. VBIJanSp43325_0272.

Phylogenomic databases

eggNOGiENOG4105E2X. Bacteria.
COG0169. LUCA.
HOGENOMiHOG000237875.
KOiK00014.
OMAiKVAIMEH.
OrthoDBiEOG64R67G.

Enzyme and pathway databases

UniPathwayiUPA00053; UER00087.
BioCyciJSP290400:GI1R-197-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00222. Shikimate_DH_AroE.
InterProiIPR016040. NAD(P)-bd_dom.
IPR011342. Shikimate_DH.
IPR013708. Shikimate_DH-bd_N.
IPR022893. Shikimate_DH_fam.
IPR006151. Shikm_DH/Glu-tRNA_Rdtase.
[Graphical view]
PfamiPF01488. Shikimate_DH. 1 hit.
PF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR00507. aroE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CCS1.

Entry informationi

Entry nameiAROE_JANSC
AccessioniPrimary (citable) accession number: Q28VZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: April 4, 2006
Last modified: December 9, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.