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Q28VD4 (SYL_JANSC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 64. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:Jann_0411
OrganismJannaschia sp. (strain CCS1) [Complete proteome] [HAMAP]
Taxonomic identifier290400 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeJannaschia

Protein attributes

Sequence length863 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 863863Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000009355

Regions

Motif41 – 5111"HIGH" region HAMAP-Rule MF_00049
Motif627 – 6315"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6301ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q28VD4 [UniParc].

Last modified April 4, 2006. Version 1.
Checksum: 5BCB6A77FEE205FF

FASTA86395,767
        10         20         30         40         50         60 
MSRYDPSMIE PKWQKAWAEA GTFTASMEGN KPKYYVLEMF PYPSGRIHIG HVRNYTMGDV 

        70         80         90        100        110        120 
IARHKKAMGH NVLHPMGFDA FGLAAENAAM DRGIHPKDWT YKNMDDMRDQ MRPLGFSIDW 

       130        140        150        160        170        180 
SRDLATCDPD YYGQQQALFI DMMEAGLIDR KNAVVNWDPV DMTVLANEQV EDGKGWRSGA 

       190        200        210        220        230        240 
EVERKELTQW FFKISDYSDE LLEALDGLDD WPAKVKLMQA NWIGKSRGLQ FAFGLVEPVG 

       250        260        270        280        290        300 
GHDRVDVYTT RPDTLLGASF VGISPDHAIA KQLESESEDI AAFCAECRKG GTTAAEVETA 

       310        320        330        340        350        360 
EKLGFDTGLK VRHPFDTAAQ LPVYIANFIL MDYGTGAIFG CPAHDERDLD FARKYDLPVV 

       370        380        390        400        410        420 
STYVPIDDEG HVLPEDGGAA YVPPKPDPVR YIRGFAGEEI QSGNDAIDAA VTFCEAEGVG 

       430        440        450        460        470        480 
QGVTKFRLRD WGLSRQRYWG CPIPVVHCDA CGVVPEKKEN LPIELPYDVT FDVPGNPLDR 

       490        500        510        520        530        540 
HPTWRNTPCP SCGAPALRET DTMDTFVDSS WYFARFTAPH ADTPTSKAEV EYWMNVDQYI 

       550        560        570        580        590        600 
GGIEHAILHL LYSRFFARAM HITGHLPRKA IEPFDALFTQ GMVTHAIYKT TDAKNRPVYH 

       610        620        630        640        650        660 
YPETVDLRDG GGYLDDGTAV EIIPSAKMSK SKNNVVDPIE IIKQYGADTA RWFVLSDSPP 

       670        680        690        700        710        720 
ERDVEWTASG AEAAYKHLGR VWALCDRISE AARSVSEEAQ PAAGEHDTAL LREMHKTIRD 

       730        740        750        760        770        780 
VTNGVDSFGF NAAIAKLYAF TNTLQKSKAS HKAQREAVMT LAQLMAPMTP HLSEDIWAHQ 

       790        800        810        820        830        840 
GGDGLIADAP WPVANEAMLV ESTVTLPIQI NGKRRSEVTV AKDASKEEVE KRALADDAVV 

       850        860 
RALDGAAPKK LIVVPGRIVN VVI 

« Hide

References

[1]"Complete sequence of chromosome of Jannaschia sp. CCS1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Chertkov O., Saunders E., Schmutz J., Larimer F. expand/collapse author list , Land M., Kyrpides N., Lykidis A., Moran M.A., Belas R., Ye W., Buchan A., Gonzalez J.M., Schell M.A., Richardson P.
Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CCS1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000264 Genomic DNA. Translation: ABD53328.1.
RefSeqYP_508353.1. NC_007802.1.

3D structure databases

ProteinModelPortalQ28VD4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290400.Jann_0411.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD53328; ABD53328; Jann_0411.
GeneID3932852.
KEGGjan:Jann_0411.
PATRIC22144436. VBIJanSp43325_0493.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAVVHCDAC.
OrthoDBEOG63Z74X.

Enzyme and pathway databases

BioCycJSP290400:GI1R-416-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_JANSC
AccessionPrimary (citable) accession number: Q28VD4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 4, 2006
Last modified: May 14, 2014
This is version 64 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries