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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Jannaschia sp. (strain CCS1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei630 – 6301ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciJSP290400:GI1R-416-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:Jann_0411
OrganismiJannaschia sp. (strain CCS1)
Taxonomic identifieri290400 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeJannaschia
ProteomesiUP000008326: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 863863Leucine--tRNA ligasePRO_1000009355Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi290400.Jann_0411.

Structurei

3D structure databases

ProteinModelPortaliQ28VD4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi41 – 5111"HIGH" regionAdd
BLAST
Motifi627 – 6315"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiREKNDWE.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q28VD4-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSRYDPSMIE PKWQKAWAEA GTFTASMEGN KPKYYVLEMF PYPSGRIHIG
60 70 80 90 100
HVRNYTMGDV IARHKKAMGH NVLHPMGFDA FGLAAENAAM DRGIHPKDWT
110 120 130 140 150
YKNMDDMRDQ MRPLGFSIDW SRDLATCDPD YYGQQQALFI DMMEAGLIDR
160 170 180 190 200
KNAVVNWDPV DMTVLANEQV EDGKGWRSGA EVERKELTQW FFKISDYSDE
210 220 230 240 250
LLEALDGLDD WPAKVKLMQA NWIGKSRGLQ FAFGLVEPVG GHDRVDVYTT
260 270 280 290 300
RPDTLLGASF VGISPDHAIA KQLESESEDI AAFCAECRKG GTTAAEVETA
310 320 330 340 350
EKLGFDTGLK VRHPFDTAAQ LPVYIANFIL MDYGTGAIFG CPAHDERDLD
360 370 380 390 400
FARKYDLPVV STYVPIDDEG HVLPEDGGAA YVPPKPDPVR YIRGFAGEEI
410 420 430 440 450
QSGNDAIDAA VTFCEAEGVG QGVTKFRLRD WGLSRQRYWG CPIPVVHCDA
460 470 480 490 500
CGVVPEKKEN LPIELPYDVT FDVPGNPLDR HPTWRNTPCP SCGAPALRET
510 520 530 540 550
DTMDTFVDSS WYFARFTAPH ADTPTSKAEV EYWMNVDQYI GGIEHAILHL
560 570 580 590 600
LYSRFFARAM HITGHLPRKA IEPFDALFTQ GMVTHAIYKT TDAKNRPVYH
610 620 630 640 650
YPETVDLRDG GGYLDDGTAV EIIPSAKMSK SKNNVVDPIE IIKQYGADTA
660 670 680 690 700
RWFVLSDSPP ERDVEWTASG AEAAYKHLGR VWALCDRISE AARSVSEEAQ
710 720 730 740 750
PAAGEHDTAL LREMHKTIRD VTNGVDSFGF NAAIAKLYAF TNTLQKSKAS
760 770 780 790 800
HKAQREAVMT LAQLMAPMTP HLSEDIWAHQ GGDGLIADAP WPVANEAMLV
810 820 830 840 850
ESTVTLPIQI NGKRRSEVTV AKDASKEEVE KRALADDAVV RALDGAAPKK
860
LIVVPGRIVN VVI
Length:863
Mass (Da):95,767
Last modified:April 4, 2006 - v1
Checksum:i5BCB6A77FEE205FF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000264 Genomic DNA. Translation: ABD53328.1.
RefSeqiWP_011453537.1. NC_007802.1.
YP_508353.1. NC_007802.1.

Genome annotation databases

EnsemblBacteriaiABD53328; ABD53328; Jann_0411.
GeneIDi3932852.
KEGGijan:Jann_0411.
PATRICi22144436. VBIJanSp43325_0493.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000264 Genomic DNA. Translation: ABD53328.1.
RefSeqiWP_011453537.1. NC_007802.1.
YP_508353.1. NC_007802.1.

3D structure databases

ProteinModelPortaliQ28VD4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290400.Jann_0411.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD53328; ABD53328; Jann_0411.
GeneIDi3932852.
KEGGijan:Jann_0411.
PATRICi22144436. VBIJanSp43325_0493.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiREKNDWE.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciJSP290400:GI1R-416-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CCS1.

Entry informationi

Entry nameiSYL_JANSC
AccessioniPrimary (citable) accession number: Q28VD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: April 4, 2006
Last modified: January 7, 2015
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.