Q28V59 (ISPE_JANSC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Jann_0486

Organism

Jannaschia sp. (strain CCS1) [Complete proteome] [HAMAP]

Taxonomic identifier

290400 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Jannaschia

Protein attributes

Sequence length	276 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 276	276	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000335719
Regions
	Nucleotide binding	94 – 104	11	ATP Potential
Sites
	Active site	13	1	By similarity
	Active site	131	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28V59 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 1CDBB9C5BC2F6F4F	FASTA	276	29,214
	10 20 30 40 50 60 MSAATFRGYA PAKVNLALHV TGRRVDGYHE LDSLVVFAGV GDRLEIAPAD ALSLTVTGPR 70 80 90 100 110 120 AEGVPDDARN LVWKAADWLA PGRGAAMTLD KHLPHAGGIG GGSADAACAL RGLAEIWDVD 130 140 150 160 170 180 VPDGAEALGA DVPVCLHGQP VRMRGIGDVL DAVPPLPPMW IVLVNAGVEV PTGAVFKAME 190 200 210 220 230 240 RVDNPPLPAP AWDGFDSFLV WLERTRNDME SSARSQAPVI GMVLERLRAL PGCRFTRMSG 250 260 270 SGGTCFGLFE TEIAARDAAL GLPKHWWVTY APVLRA

References

[1]

"Complete sequence of chromosome of Jannaschia sp. CCS1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Chertkov O., Saunders E., Schmutz J., Larimer F. , Land M., Kyrpides N., Lykidis A., Moran M.A., Belas R., Ye W., Buchan A., Gonzalez J.M., Schell M.A., Richardson P. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CCS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000264 Genomic DNA. Translation: ABD53403.1.
RefSeq	YP_508428.1. NC_007802.1.
3D structure databases
ProteinModelPortal	Q28V59.
ModBase	Search...
Protein-protein interaction databases
STRING	Q28V59.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3932927.
GenomeReviews	Gene locus Jann_0486 in contig CP000264_GR.
KEGG	jan:Jann_0486.
PATRIC	22144588. VBIJanSp43325_0569.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	CQLARMS.
PhylomeDB	Q28V59.
Enzyme and pathway databases
BioCyc	JSP290400:JANN_0486-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_JANSC

Accession

Primary (citable) accession number: Q28V59

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	April 4, 2006
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program