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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Jannaschia sp. (strain CCS1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei67 – 671S-adenosyl-L-methionineUniRule annotation
Binding sitei92 – 921S-adenosyl-L-methionineUniRule annotation
Binding sitei119 – 1191S-adenosyl-L-methionineUniRule annotation
Active sitei141 – 1411By similarity
Binding sitei141 – 1411S-adenosyl-L-methionineUniRule annotation
Binding sitei145 – 1451SubstrateUniRule annotation
Binding sitei177 – 1771SubstrateUniRule annotation

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciJSP290400:GI1R-714-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Jann_0707
OrganismiJannaschia sp. (strain CCS1)
Taxonomic identifieri290400 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeJannaschia
ProteomesiUP000008326 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 237237tRNA (guanine-N(7)-)-methyltransferasePRO_0000288160Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi290400.Jann_0707.

Structurei

3D structure databases

ProteinModelPortaliQ28UI8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni214 – 2174Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073969.
KOiK03439.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q28UI8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDPHPNRPH RNFYGRRKGH DLRDSQERYL AEDLAKLSPG AVDWDVNPDR
60 70 80 90 100
TPLDLKERFG GKDIWLEVGF GGGEHLVHQA ASNPDVAIIG CEPYINGVAM
110 120 130 140 150
LLGKIRAAGV GNLAVYPGDA RDLFDVLPKG SVAKAFLLYP DPWPKARHHR
160 170 180 190 200
RRFVTTEHLE PLHRAMRPGA EFRVATDIED YVRQTMEEVP KAGFEWLAEA
210 220 230
ASDWREPWCD WVSTRYEQKA LREGRVPHYM TFQKRSL
Length:237
Mass (Da):27,122
Last modified:May 29, 2007 - v2
Checksum:i7A01AC4EF14C8C80
GO

Sequence cautioni

The sequence ABD53624.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000264 Genomic DNA. Translation: ABD53624.1. Different initiation.
RefSeqiWP_011453832.1. NC_007802.1.
YP_508649.1. NC_007802.1.

Genome annotation databases

EnsemblBacteriaiABD53624; ABD53624; Jann_0707.
GeneIDi3933150.
KEGGijan:Jann_0707.
PATRICi22145050. VBIJanSp43325_0798.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000264 Genomic DNA. Translation: ABD53624.1. Different initiation.
RefSeqiWP_011453832.1. NC_007802.1.
YP_508649.1. NC_007802.1.

3D structure databases

ProteinModelPortaliQ28UI8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi290400.Jann_0707.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABD53624; ABD53624; Jann_0707.
GeneIDi3933150.
KEGGijan:Jann_0707.
PATRICi22145050. VBIJanSp43325_0798.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073969.
KOiK03439.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciJSP290400:GI1R-714-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CCS1.

Entry informationi

Entry nameiTRMB_JANSC
AccessioniPrimary (citable) accession number: Q28UI8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: May 29, 2007
Last modified: January 7, 2015
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.