Q28TK3 (GLO2_JANSC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxyacylglutathione hydrolase
EC=3.1.2.6

Alternative name(s):
Glyoxalase II
Short name=Glx II

Gene names

Name:	gloB
Ordered Locus Names:	Jann_1042

Organism

Jannaschia sp. (strain CCS1) [Complete proteome] [HAMAP]

Taxonomic identifier

290400 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Jannaschia

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity. HAMAP-Rule MF_01374
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate. HAMAP-Rule MF_01374
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2. HAMAP-Rule MF_01374
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glutathione biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	Hydroxyacylglutathione hydrolase HAMAP-Rule MF_01374		PRO_0000309652
Sites
	Metal binding	57	1	Zinc 1 By similarity
	Metal binding	59	1	Zinc 1 By similarity
	Metal binding	61	1	Zinc 2 By similarity
	Metal binding	62	1	Zinc 2 By similarity
	Metal binding	115	1	Zinc 1 By similarity
	Metal binding	134	1	Zinc 1 By similarity
	Metal binding	134	1	Zinc 2 By similarity
	Metal binding	172	1	Zinc 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28TK3 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: A1789C7C568064EF	FASTA	256	27,033
	10 20 30 40 50 60 MADPQIITIP CLQDNYAFLL HDPSTGATAC IDVPDAAPIQ AALGAQGWTL TDILLTHHHW 70 80 90 100 110 120 DHIDGVPALV EATGAKVWGA AADAHRLPPL DHALAEGDTI RIGALEGDVM DVSGHTVGHV 130 140 150 160 170 180 AFHFPAATAV FTADSLMALG CGRLFEGTPA QMHGSLEKLK MLPPGTLVCS GHEYTASNAR 190 200 210 220 230 240 FALTIEPDNP DLIFRVERIT AARAAGQPTV PSTLAEELAT NPFLRADAPT VAAPLDMEGA 250 DPVDVFTRIR AQKDSF

References

[1]

"Complete sequence of chromosome of Jannaschia sp. CCS1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Chertkov O., Saunders E., Schmutz J., Larimer F. , Land M., Kyrpides N., Lykidis A., Moran M.A., Belas R., Ye W., Buchan A., Gonzalez J.M., Schell M.A., Richardson P. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CCS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000264 Genomic DNA. Translation: ABD53959.1.
RefSeq	YP_508984.1. NC_007802.1.
3D structure databases
ProteinModelPortal	Q28TK3.
ModBase	Search...
Protein-protein interaction databases
STRING	290400.Jann_1042.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABD53959; ABD53959; Jann_1042.
GeneID	3933486.
KEGG	jan:Jann_1042.
PATRIC	22145756. VBIJanSp43325_1150.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0491.
HOGENOM	HOG000058041.
KO	K01069.
OMA	PFMRVHE.
ProtClustDB	CLSK836335.
Enzyme and pathway databases
BioCyc	JSP290400:GI1R-1095-MONOMER.
UniPathway	UPA00619; UER00676.
Family and domain databases
HAMAP	MF_01374. Glyoxalase_2.
InterPro	IPR001279. Beta-lactamas-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
Pfam	PF00753. Lactamase_B. 1 hit. [Graphical view]
PIRSF	PIRSF005457. Glx. 1 hit.
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_JANSC

Accession

Primary (citable) accession number: Q28TK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	April 4, 2006
Last modified:	May 1, 2013
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program