Reviewed, UniProtKB/Swiss-Prot Q28RK3 (PYRC_JANSC)

Names and origin

Protein names

Recommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3

Gene names

Name:	pyrC
Ordered Locus Names:	Jann_1742

Organism

Jannaschia sp. (strain CCS1) [Complete proteome] [HAMAP]

Taxonomic identifier

290400 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Jannaschia

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the DHOase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 347	347	Dihydroorotase		PRO_1000024019
Sites
	Metal binding	14	1	Zinc 1 By similarity
	Metal binding	16	1	Zinc 1 By similarity
	Metal binding	100	1	Zinc 1; via carbamate group By similarity
	Metal binding	100	1	Zinc 2; via carbamate group By similarity
	Metal binding	137	1	Zinc 2 By similarity
	Metal binding	175	1	Zinc 2 By similarity
	Metal binding	248	1	Zinc 1 By similarity
Amino acid modifications
	Modified residue	100	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28RK3-1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: D0BA18015744F768	FASTA	347	37,943
	10 20 30 40 50 60 MTQSLTIRRP DDWHLHLRDG AMMRAVLPET ARHFARAIIM PNLVPPVVTA QDALAYRDRI 70 80 90 100 110 120 EAALPDGADF TPLMTLYLTE QTDPDDVEAA FADGLIHAVK LYPAGATTNS QSGVATLKNV 130 140 150 160 170 180 YPVLERMAQI GLPLCIHGEV TDPQVDIFDR EQAFIDTVLI PLRADLPDLK VTLEHITTSH 190 200 210 220 230 240 GIDYVVGADG DIAGTITTHH LMINRNHMLV GGIRPHYYCL PIVKRASHQH ALRRAATSGH 250 260 270 280 290 300 PRFFLGTDSA PHLDGAKETA CGCAGVFSAT NTMSCLAHVF DEDGALVNLE AFTSLNGPAR 310 320 330 340 YGLAPNAATM TLEKRDHPTN YPAKFDTDDG PLTLFDPSTP LYWHVKD

References

[1]

"Complete sequence of chromosome of Jannaschia sp. CCS1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Chertkov O., Saunders E., Schmutz J., Larimer F. , Land M., Kyrpides N., Lykidis A., Moran M.A., Belas R., Ye W., Buchan A., Gonzalez J.M., Schell M.A., Richardson P. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000264 Genomic DNA. Translation: ABD54659.1.
RefSeq	YP_509684.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3934190.
GenomeReviews	Gene locus Jann_1742 in contig CP000264_GR.
KEGG	jan:Jann_1742.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q28RK3.
Enzyme and pathway databases
BioCyc	JSP290400:JANN_1742-MON.
Family and domain databases
HAMAP	MF_00219. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR004721. DHOdimr. IPR002195. Dihydroorotase_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. [Graphical view]
PIRSF	PIRSF001237. DHOdimr. 1 hit.
TIGRFAMs	TIGR00856. pyrC_dimer. 1 hit.
PROSITE	PS00482. DIHYDROOROTASE_1. 1 hit. PS00483. DIHYDROOROTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRC_JANSC

Accession

Primary (citable) accession number: Q28RK3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	April 4, 2006
Last modified:	November 25, 2008
This is version 19 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)