Reviewed, UniProtKB/Swiss-Prot Q28RJ3 (URE1_JANSC)

Names and origin

Protein names

Recommended name:
    Urease subunit alpha
    EC=3.5.1.5
Alternative name(s):
    Urea amidohydrolase subunit alpha

Gene names

Name:	ureC
Ordered Locus Names:	Jann_1752

Organism

Jannaschia sp. (strain CCS1) [Complete proteome] [HAMAP]

Taxonomic identifier

290400 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Jannaschia

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Urea + H2O = CO2 + 2 NH3. HAMAP MF_01953
Cofactor	Binds 2 nickel ions per subunit By similarity.
Pathway	Nitrogen metabolism; urea degradation; CO(2) and NH(3) from urea (urease route): step 1/1. HAMAP MF_01953
Subunit structure	Heterotrimer of ureA (gamma), ureB (beta) and ureC (alpha) subunits. Three heterotrimers associate to form the active enzyme By similarity.
Subcellular location	Cytoplasm By similarity.
Post-translational modification	Carbamylation allows a single lysine to coordinate two nickel ions By similarity.
Sequence similarities	Belongs to the urease family. Contains 1 urease domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Nickel
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	urea metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW urease activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 569	569	Urease subunit alpha HAMAP MF_01953		PRO_0000239877
Regions
	Domain	131 – 569	439	Urease
Sites
	Active site	322	1	Proton donor By similarity
	Metal binding	136	1	Nickel 2 By similarity
	Metal binding	138	1	Nickel 2 By similarity
	Metal binding	219	1	Nickel 1; via carbamate group By similarity
	Metal binding	219	1	Nickel 2; via carbamate group By similarity
	Metal binding	248	1	Nickel 1 By similarity
	Metal binding	274	1	Nickel 1 By similarity
	Metal binding	362	1	Nickel 2 By similarity
	Binding site	221	1	Substrate By similarity
Amino acid modifications
	Modified residue	219	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28RJ3-1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 3B58F0DF91AAAF21	FASTA	569	61,338
	10 20 30 40 50 60 MPTKIPRNDY AAMYGPTTGD RVRLADTDLI IEVERDLTTY GEEVKFGGGK VIRDGMGQSQ 70 80 90 100 110 120 VTRAQCAVDT VITNALIVDH SGIYKADVGL KDGRIHKIGK AGNPDTQPNV DIIIGPGTEA 130 140 150 160 170 180 IAGEGRILTA GGFDAHIHFI CPQQIEDSLH SGITTMLGGG TGPAHGTLAT TCTPGPWHIG 190 200 210 220 230 240 RMLQSLDAFP MNFGLSCKGN ASQPEALVEM VSAGACAMKL HEDWGTTPGA IDCCLSVADE 250 260 270 280 290 300 MDVQVMIHTD TLNESGFVEN TLAAIGDRTI HAFHTEGAGG GHAPDIMKVV GYENILPSST 310 320 330 340 350 360 NPTMPYTVNT LEEHLDMLMV CHHLDKSIPE DVAFAESRIR KETIAAEDIL HDMGAFSVMA 370 380 390 400 410 420 SDSQAMGRVG EVIIRTWQTA DKMKKQRGRL AEETGDNDNA RVMRYIAKYT INPALVHGMS 430 440 450 460 470 480 REIGSIEEGK RADLVLWSPA FFGVKPEMSL IGGTIVMAQM GDPNASIPTP QPVYSRPMFG 490 500 510 520 530 540 AFGRAVERSA VLFVSEAAQA AGIGDQLGLA KDTLAVKSTR DIRKSDMIHN SFRPDIHVDP 550 560 ETYDVRANGE LLTCEPATDL PLAQRYFMY

References

[1]

"Complete sequence of chromosome of Jannaschia sp. CCS1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Chertkov O., Saunders E., Schmutz J., Larimer F. , Land M., Kyrpides N., Lykidis A., Moran M.A., Belas R., Ye W., Buchan A., Gonzalez J.M., Schell M.A., Richardson P. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000264 Genomic DNA. Translation: ABD54669.1.
RefSeq	YP_509694.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q28RJ3.
Genome annotation databases
GeneID	3934200.
GenomeReviews	Gene locus Jann_1752 in contig CP000264_GR.
KEGG	jan:Jann_1752.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q28RJ3.
OMA	SHIHFIC.
Enzyme and pathway databases
BioCyc	JSP290400:JANN_1752-MON.
Family and domain databases
HAMAP	MF_01953. [Tree]
InterPro	IPR006680. Amidohydro_1. IPR011612. Urease_alpha_N. IPR005848. Urease_asu. IPR017951. Urease_asu_c. IPR017952. Urease_asu_core. IPR017950. Urease_asu_CS. [Graphical view]
Pfam	PF01979. Amidohydro_1. 1 hit. PF00449. Urease_alpha. 1 hit. [Graphical view]
PRINTS	PR01752. UREASE.
TIGRFAMs	TIGR01792. urease_alph. 1 hit.
PROSITE	PS01120. UREASE_1. 1 hit. PS00145. UREASE_2. 1 hit. PS51368. UREASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URE1_JANSC

Accession

Primary (citable) accession number: Q28RJ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 13, 2006
Last sequence update:	April 4, 2006
Last modified:	November 3, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)