Reviewed, UniProtKB/Swiss-Prot Q28RA3 (SYE1_JANSC)

Names and origin

Protein names

Recommended name:
    Glutamyl-tRNA synthetase 1
    EC=6.1.1.17
Alternative name(s):
    Glutamate--tRNA ligase 1
      Short name=GluRS 1

Gene names

Name:	gltX1
Ordered Locus Names:	Jann_1842

Organism

Jannaschia sp. (strain CCS1) [Complete proteome] [HAMAP]

Taxonomic identifier

290400 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Jannaschia

Protein attributes

Sequence length	470 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity. HAMAP MF_00022
Subcellular location	Cytoplasm HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 470	470	Glutamyl-tRNA synthetase 1 HAMAP MF_00022		PRO_0000330975
Regions
	Motif	15 – 25	11	"HIGH" region HAMAP MF_00022
	Motif	241 – 245	5	"KMSKS" region HAMAP MF_00022
Sites
	Binding site	244	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28RA3-1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: EF92569AE1D3EFEE	FASTA	470	51,878
	10 20 30 40 50 60 MSSDAASPVV TRIAPSPTGT MHIGTARTGL FNWLFARKHG GKFVLRIEDT DRERSTPEAT 70 80 90 100 110 120 QAILDGMAWL GLDYDGEAVS QFARADRHRE VAEQMLAEGH AYKCFSTQDE IAAFRDAAKA 130 140 150 160 170 180 DGRSTLFLSP WREASDHPDA PYVIRLKAPR DGTTVIRDRV QSDVTIQNAT LDDMILLRSD 190 200 210 220 230 240 GTPTYMHAVV VDDHDMGVTH VVRGDDHLNN AARQIQIYAA MGWAEPVWSH IPLIHGDDGK 250 260 270 280 290 300 KLSKRHGATG VMEYAEMGIP AAAMRNYLTR LGWSHGDEEL FTDEQARDWF DLDGIGKSPS 310 320 330 340 350 360 RLDMKKLANV TGWHIARTDD ADLVAQIEGY LALTGAPALS QAQHDGLTRA MYCLKDRAKS 370 380 390 400 410 420 LPDLLDKGRF VLENRPFDYD EKSVTMLDPV SRSILRELTA QLQNARWTRD TLEGVLNAAA 430 440 450 460 470 DSHGLKFGKL AGPLRAALSG RSVSPSVFDM MLVIGRDETL ARLDEAASLT

References

[1]

"Complete sequence of chromosome of Jannaschia sp. CCS1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Chertkov O., Saunders E., Schmutz J., Larimer F. , Land M., Kyrpides N., Lykidis A., Moran M.A., Belas R., Ye W., Buchan A., Gonzalez J.M., Schell M.A., Richardson P. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000264 Genomic DNA. Translation: ABD54759.1.
RefSeq	YP_509784.1.
3D structure databases
SMR	Q28RA3. Positions 9-468.
ModBase	Search...
Protein-protein interaction databases
STRING	Q28RA3.
Genome annotation databases
GeneID	3934293.
GenomeReviews	Gene locus Jann_1842 in contig CP000264_GR.
KEGG	jan:Jann_1842.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	DLVIQRS.
PhylomeDB	Q28RA3.
Enzyme and pathway databases
BioCyc	JSP290400:JANN_1842-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ic_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00464. gltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE1_JANSC

Accession

Primary (citable) accession number: Q28RA3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	April 4, 2006
Last modified:	February 9, 2010
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)