Reviewed, UniProtKB/Swiss-Prot Q28PE7 (TRMFO_JANSC)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO
    EC=2.1.1.74
Alternative name(s):
    Folate-dependent tRNA (uracil-5-)-methyltransferase
    Folate-dependent tRNA(M-5-U54)-methyltransferase

Gene names

Name:	trmFO
Ordered Locus Names:	Jann_2498

Organism

Jannaschia sp. (strain CCS1) [Complete proteome] [HAMAP]

Taxonomic identifier

290400 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Jannaschia

Protein attributes

Sequence length	458 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mnmG family. TrmFO subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC tRNA (uracil-5-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 458	458	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO HAMAP MF_01037		PRO_0000346345
Regions
	Nucleotide binding	11 – 16	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28PE7-1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: C5BC5B0F50820C58	FASTA	458	49,479
	10 20 30 40 50 60 MTQNTQLTII GGGMAGSEAA WQAANLGVNV RLIEMRPKVE TFAHRTGNLA EMVCSNSFRS 70 80 90 100 110 120 DDSEQNAVGL LHWEMRAANS VIMHTADSHK LPAGGALAVD RDPFAEAVTA KLHAHPNIEI 130 140 150 160 170 180 TYGEVTDLPA AGPTIIATGP LTGSALADAI AREAGQDALA FFDAIAPIVY ADSIDMDIAW 190 200 210 220 230 240 RQSRYDKGDT LEEQQAYINC PLTRDQYEAF IDALLSADKT QFKDGETAGY FDGCLPIEVM 250 260 270 280 290 300 AERGRETLRF GPMKPVGLTN PHDPQTKAYA VVQLRRDNAL GTLYNIVGFQ TKMTYGAQKQ 310 320 330 340 350 360 VFAMIPGLQE ASFARLGGIH RNTFINSPTL LDDQMRLRSK PHIRFAGQIT GVEGYVESAS 370 380 390 400 410 420 MGLLAGRMAA AEILGETLPD LPDTTAMGAL VTHITGGADA KTFQPMNVNF GLFPPVEGLK 430 440 450 GGRRGRKDRY KAYTDRAKAA WTNWLTPQTS LGGSQAAE

References

[1]

"Complete sequence of chromosome of Jannaschia sp. CCS1." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Chertkov O., Saunders E., Schmutz J., Larimer F. , Land M., Kyrpides N., Lykidis A., Moran M.A., Belas R., Ye W., Buchan A., Gonzalez J.M., Schell M.A., Richardson P. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000264 Genomic DNA. Translation: ABD55415.1.
RefSeq	YP_510440.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3934957.
GenomeReviews	Gene locus Jann_2498 in contig CP000264_GR.
KEGG	jan:Jann_2498.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q28PE7.
OMA	Q28PE7. MKPVGLT.
Enzyme and pathway databases
BioCyc	JSP290400:JANN_2498-MON.
Family and domain databases
HAMAP	MF_01037. [Tree]
InterPro	IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR004417. Gid. IPR002218. GIDA-rel. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11806. GIDA. 1 hit.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
ProDom	PD003738. GIDA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00137. gid_trmFO. 1 hit.
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMFO_JANSC

Accession

Primary (citable) accession number: Q28PE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	April 4, 2006
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)