Q28PE7 (TRMFO_JANSC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
EC=2.1.1.74

Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase
Folate-dependent tRNA(M-5-U54)-methyltransferase

Gene names

Name:	trmFO
Ordered Locus Names:	Jann_2498

Organism

Jannaschia sp. (strain CCS1) [Complete proteome] [HAMAP]

Taxonomic identifier

290400 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Jannaschia

Protein attributes

Sequence length	458 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity. HAMAP MF_01037
Catalytic activity	5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037
Cofactor	FAD By similarity. HAMAP MF_01037
Subcellular location	Cytoplasm By similarity HAMAP MF_01037.
Sequence similarities	Belongs to the MnmG family. TrmFO subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 458	458	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO HAMAP MF_01037		PRO_0000346345
Regions
	Nucleotide binding	11 – 16	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28PE7 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: C5BC5B0F50820C58	FASTA	458	49,479
	10 20 30 40 50 60 MTQNTQLTII GGGMAGSEAA WQAANLGVNV RLIEMRPKVE TFAHRTGNLA EMVCSNSFRS 70 80 90 100 110 120 DDSEQNAVGL LHWEMRAANS VIMHTADSHK LPAGGALAVD RDPFAEAVTA KLHAHPNIEI 130 140 150 160 170 180 TYGEVTDLPA AGPTIIATGP LTGSALADAI AREAGQDALA FFDAIAPIVY ADSIDMDIAW 190 200 210 220 230 240 RQSRYDKGDT LEEQQAYINC PLTRDQYEAF IDALLSADKT QFKDGETAGY FDGCLPIEVM 250 260 270 280 290 300 AERGRETLRF GPMKPVGLTN PHDPQTKAYA VVQLRRDNAL GTLYNIVGFQ TKMTYGAQKQ 310 320 330 340 350 360 VFAMIPGLQE ASFARLGGIH RNTFINSPTL LDDQMRLRSK PHIRFAGQIT GVEGYVESAS 370 380 390 400 410 420 MGLLAGRMAA AEILGETLPD LPDTTAMGAL VTHITGGADA KTFQPMNVNF GLFPPVEGLK 430 440 450 GGRRGRKDRY KAYTDRAKAA WTNWLTPQTS LGGSQAAE

References

[1]

"Complete sequence of chromosome of Jannaschia sp. CCS1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Chertkov O., Saunders E., Schmutz J., Larimer F. , Land M., Kyrpides N., Lykidis A., Moran M.A., Belas R., Ye W., Buchan A., Gonzalez J.M., Schell M.A., Richardson P. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CCS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000264 Genomic DNA. Translation: ABD55415.1.
RefSeq	YP_510440.1. NC_007802.1.
3D structure databases
ProteinModelPortal	Q28PE7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q28PE7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3934957.
GenomeReviews	Gene locus Jann_2498 in contig CP000264_GR.
KEGG	jan:Jann_2498.
PATRIC	22148782. VBIJanSp43325_2652.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1206.
HOGENOM	HBG285041.
OMA	RFAGQIT.
ProtClustDB	PRK05335.
Enzyme and pathway databases
BioCyc	JSP290400:JANN_2498-MONOMER.
Family and domain databases
HAMAP	MF_01037. TrmFO. [Tree]
InterPro	IPR004417. Folate-dep_Ribothymidyl_synth. IPR002218. GIDA-rel. IPR020595. GIDA-rel_CS. [Graphical view]
KO	K04094.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00137. Gid_trmFO. 1 hit.
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMFO_JANSC

Accession

Primary (citable) accession number: Q28PE7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	April 4, 2006
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program