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Q28NP2 (DDL_JANSC) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:Jann_2753
OrganismJannaschia sp. (strain CCS1) [Complete proteome] [HAMAP]
Taxonomic identifier290400 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhodobacteralesRhodobacteraceaeJannaschia

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 316316D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000341113

Regions

Domain112 – 310199ATP-grasp
Nucleotide binding139 – 18951ATP By similarity

Sites

Metal binding2611Magnesium or manganese 1 By similarity
Metal binding2771Magnesium or manganese 1 By similarity
Metal binding2771Magnesium or manganese 2 By similarity
Metal binding2791Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q28NP2 [UniParc].

Last modified April 4, 2006. Version 1.
Checksum: 4321E2611B438E3D

FASTA31633,706
        10         20         30         40         50         60 
MAGLSSLPSR VVVLMGGPSA EREVSLSTGR GCAEALRGEG FDVIEVDPGV THAGAELCAR 

        70         80         90        100        110        120 
LQEIQPDVVF NALHGRWGED GCVQGLLEWM RLPYTHSGVL SSALAMDKTR AKTALKAHGL 

       130        140        150        160        170        180 
PVVDSIIAPK EAVVAAHVMA TPYVVKPNNE GSSVGVYLVN EAANGPPHLS DDMPDEVMVE 

       190        200        210        220        230        240 
TYAPGRELTV SVLGDAPGDP GALTVTDILT DGWYDYDAKY KPGGSRHVVP AQIPQEIWDA 

       250        260        270        280        290        300 
CMDMAVRAHT ILGCKGVSRT DYRWDEARGL DGLIILEVNT QPGMTPTSLT PEQGEAVGMS 

       310 
FGKLCRWLVE DATCDR 

« Hide

References

[1]"Complete sequence of chromosome of Jannaschia sp. CCS1."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Chertkov O., Saunders E., Schmutz J., Larimer F. expand/collapse author list , Land M., Kyrpides N., Lykidis A., Moran M.A., Belas R., Ye W., Buchan A., Gonzalez J.M., Schell M.A., Richardson P.
Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: CCS1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000264 Genomic DNA. Translation: ABD55670.1.
RefSeqYP_510695.1. NC_007802.1.

3D structure databases

ProteinModelPortalQ28NP2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING290400.Jann_2753.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABD55670; ABD55670; Jann_2753.
GeneID3935217.
KEGGjan:Jann_2753.
PATRIC22149338. VBIJanSp43325_2925.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMACSGYSRV.
OrthoDBEOG6ND0KB.

Enzyme and pathway databases

BioCycJSP290400:GI1R-2781-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_JANSC
AccessionPrimary (citable) accession number: Q28NP2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 10, 2008
Last sequence update: April 4, 2006
Last modified: May 14, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways