Q28L59 (SYE2_JANSC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 2
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase 2
Short name=GluRS 2

Gene names

Name:	gltX2
Ordered Locus Names:	Jann_3636

Organism

Jannaschia sp. (strain CCS1) [Complete proteome] [HAMAP]

Taxonomic identifier

290400 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Jannaschia

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Glutamate--tRNA ligase 2 HAMAP MF_00022_B		PRO_0000367693
Regions
	Motif	17 – 27	11	"HIGH" region HAMAP MF_00022_B
	Motif	248 – 252	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	251	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28L59 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 4BFCEB83BCAA718F	FASTA	449	49,517
	10 20 30 40 50 60 MSAPMSASTS VVTRFAPSPT GFLHVGNLRT ALFNFLIAKK AGGEFILRLD DTDPERSTQA 70 80 90 100 110 120 FADAIQEDME WLGLTWDRIE RQSDRFDRYA AAADELRAKN RFYEAWETPT ELDLKRKKQL 130 140 150 160 170 180 NMHQPPVYDR AALALTDDQK EALRAERGQG VWRFKLDHQR IEWTDGILGD LSIDAASVSD 190 200 210 220 230 240 PVLIRADGQV LYTIASVVDD TDMGVTHVVR GSDHVTNTAT QIQIMEALGK SAPSFAHHSL 250 260 270 280 290 300 LTGPQGEALS KRLGTLALRD LREAGMEPMA LLSHMARIGS SQPVELAGSV EELAEGFDLN 310 320 330 340 350 360 HFGSAPTKFD VEDLWPLTAS VLHGTAFEDV AGEIAALGVP ADIAPAFWEV ARANIGKRAE 370 380 390 400 410 420 IADWWALFRD GATPLVADED REFVAQAFAM LPDLPYDETT WSNWTSAVKE ATGRKGKGLF 430 440 MPLRKAVTGR ERGPEMADVM RLMQVKPTL

References

[1]

"Complete sequence of chromosome of Jannaschia sp. CCS1." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Pitluck S., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Chertkov O., Saunders E., Schmutz J., Larimer F. , Land M., Kyrpides N., Lykidis A., Moran M.A., Belas R., Ye W., Buchan A., Gonzalez J.M., Schell M.A., Richardson P. Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CCS1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000264 Genomic DNA. Translation: ABD56553.1.
RefSeq	YP_511578.1. NC_007802.1.
3D structure databases
ProteinModelPortal	Q28L59.
ModBase	Search...
Protein-protein interaction databases
STRING	Q28L59.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3936114.
GenomeReviews	Gene locus Jann_3636 in contig CP000264_GR.
KEGG	jan:Jann_3636.
PATRIC	22151178. VBIJanSp43325_3834.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	DQERIEW.
ProtClustDB	PRK12558.
Enzyme and pathway databases
BioCyc	JSP290400:JANN_3636-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE2_JANSC

Accession

Primary (citable) accession number: Q28L59

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	April 4, 2006
Last modified:	January 25, 2012
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program