Reviewed, UniProtKB/Swiss-Prot Q28IU1 (DHB12_XENTR)

Names and origin

Protein names

Recommended name:
    Estradiol 17-beta-dehydrogenase 12
    EC=1.1.1.62
Alternative name(s):
    17-beta-hydroxysteroid dehydrogenase 12
      Short name=17-beta-HSD 12

Gene names

Name:	hsd17b12
ORF Names:	TNeu053h21.1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation By similarity.
Catalytic activity	Estradiol-17-beta + NAD(P)(+) = estrone + NAD(P)H.
Pathway	Steroid biosynthesis; estrogen biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane proteinBy similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	binding Inferred from electronic annotation. Source: InterPro estradiol 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Estradiol 17-beta-dehydrogenase 12		PRO_0000248375
Regions
	Transmembrane	17 – 37	21	Potential
	Transmembrane	189 – 209	21	Potential
	Transmembrane	283 – 303	21	Potential
	Nucleotide binding	56 – 85	30	NADP By similarity
Sites
	Active site	209	1	Proton acceptor By similarity
	Binding site	196	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28IU1-1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: C216ECF085D4AF14	FASTA	320	35,101
	10 20 30 40 50 60 MATESLAEVP VPGCNCFWYL GVVAAVWWGL RAAWCLLDGA RVWVLGSGAQ VGPRIGKWAV 70 80 90 100 110 120 VTGATDGIGK AYAEELAKRG MNIVLISRSP EKLEEVAKQI KEKFKVETKI IAADFGKPTE 130 140 150 160 170 180 IYGRIESGLR DLEIGVLVNN VGVSYEHPEY FLEIPDLENT LDKMININIT SVCQMTRLVL 190 200 210 220 230 240 PGMLGRGRGV ILNISSASGM YPVPLLTVYS ATKAFVDFFS RGLQAEYRSK GVTVQSVLPF 250 260 270 280 290 300 YVATKLAKIR KPTWDKPSPE TYVQSALNTV GLQTQTNGYL PHAIMGWIST SLVPVSTAIS 310 320 LGMKMNKGLR ARFLKRAKQK

References

[1]	Sanger Xenopus tropicalis EST/cDNA project Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Neurula.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR760225 mRNA. Translation: CAJ83357.1.
RefSeq	NP_001017234.1.
UniGene	Str.10270
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	549988.
KEGG	xtr:549988.
Organism-specific databases
Xenbase	XB-FEAT-945228. hsd17b12.
Phylogenomic databases
HOVERGEN	Q28IU1.
Family and domain databases
InterPro	IPR002198. DHase_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DHase. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR19410. ADH_short_C2. 1 hit.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHB12_XENTR

Accession

Primary (citable) accession number: Q28IU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	April 4, 2006
Last modified:	November 25, 2008
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project