Q28IU1 (DHB12_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Estradiol 17-beta-dehydrogenase 12
EC=1.1.1.62

Alternative name(s):
17-beta-hydroxysteroid dehydrogenase 12
Short name=17-beta-HSD 12

Gene names

Name:	hsd17b12
ORF Names:	TNeu053h21.1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation By similarity.
Catalytic activity	Estradiol-17-beta + NAD(P)+ = estrone + NAD(P)H.
Pathway	Steroid biosynthesis; estrogen biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Ontologies

Keywords
Biological process	Lipid synthesis Steroid biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	steroid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	estradiol 17-beta-dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	Estradiol 17-beta-dehydrogenase 12		PRO_0000248375
Regions
	Transmembrane	17 – 37	21	Helical; Potential
	Transmembrane	189 – 209	21	Helical; Potential
	Transmembrane	283 – 303	21	Helical; Potential
	Nucleotide binding	56 – 85	30	NADP By similarity
Sites
	Active site	209	1	Proton acceptor By similarity
	Binding site	196	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28IU1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: C216ECF085D4AF14	FASTA	320	35,101
	10 20 30 40 50 60 MATESLAEVP VPGCNCFWYL GVVAAVWWGL RAAWCLLDGA RVWVLGSGAQ VGPRIGKWAV 70 80 90 100 110 120 VTGATDGIGK AYAEELAKRG MNIVLISRSP EKLEEVAKQI KEKFKVETKI IAADFGKPTE 130 140 150 160 170 180 IYGRIESGLR DLEIGVLVNN VGVSYEHPEY FLEIPDLENT LDKMININIT SVCQMTRLVL 190 200 210 220 230 240 PGMLGRGRGV ILNISSASGM YPVPLLTVYS ATKAFVDFFS RGLQAEYRSK GVTVQSVLPF 250 260 270 280 290 300 YVATKLAKIR KPTWDKPSPE TYVQSALNTV GLQTQTNGYL PHAIMGWIST SLVPVSTAIS 310 320 LGMKMNKGLR ARFLKRAKQK

References

[1]	Sanger Xenopus tropicalis EST/cDNA project Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Neurula.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR760225 mRNA. Translation: CAJ83357.1.
RefSeq	NP_001017234.1. NM_001017234.2.
UniGene	Str.10270.
3D structure databases
ProteinModelPortal	Q28IU1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q28IU1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSXETT00000048094; ENSXETP00000048094; ENSXETG00000022229.
GeneID	549988.
KEGG	xtr:549988.
Organism-specific databases
CTD	51144.
Xenbase	XB-GENE-945229. hsd17b12.
Phylogenomic databases
GeneTree	ENSGT00390000010069.
HOVERGEN	HBG005478.
OMA	NKSTRAR.
OrthoDB	EOG4HMJB0.
Gene expression databases
Bgee	Q28IU1.
Family and domain databases
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00044. K10251.
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHB12_XENTR

Accession

Primary (citable) accession number: Q28IU1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2006
Last sequence update:	April 4, 2006
Last modified:	December 14, 2011
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program