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Q28H76

- TRMBA_XENTR

UniProt

Q28H76 - TRMBA_XENTR

Protein

tRNA (guanine-N(7)-)-methyltransferase A

Gene

mettl1-A

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 60 (01 Oct 2014)
      Sequence version 2 (05 May 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei86 – 861S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Binding sitei162 – 1621S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
    Active sitei165 – 1651UniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB
    2. tRNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. RNA (guanine-N7)-methylation Source: GOC
    2. tRNA modification Source: UniProtKB

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    RNA-binding, S-adenosyl-L-methionine, tRNA-binding

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferase AUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    Methyltransferase-like protein 1-AUniRule annotation
    tRNA (guanine(46)-N(7))-methyltransferase AUniRule annotation
    tRNA(m7G46)-methyltransferase AUniRule annotation
    Gene namesi
    Name:mettl1-A
    ORF Names:TEgg045g13.1
    OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
    Taxonomic identifieri8364 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
    ProteomesiUP000008143: Unplaced

    Organism-specific databases

    XenbaseiXB-GENE-1000662. mettl1.

    Subcellular locationi

    Nucleus UniRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 273273tRNA (guanine-N(7)-)-methyltransferase APRO_0000370560Add
    BLAST

    Proteomic databases

    PRIDEiQ28H76.

    Expressioni

    Gene expression databases

    BgeeiQ28H76.

    Interactioni

    Subunit structurei

    Forms a complex with wdr4.UniRule annotation

    Protein-protein interaction databases

    STRINGi8364.ENSXETP00000021255.

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni109 – 1102S-adenosyl-L-methionine bindingUniRule annotation
    Regioni142 – 1432S-adenosyl-L-methionine bindingUniRule annotation
    Regioni240 – 2423S-adenosyl-L-methionine bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    GeneTreeiENSGT00390000017840.
    HOGENOMiHOG000260965.
    InParanoidiQ6DFR8.
    KOiK03439.
    OrthoDBiEOG7PS1GC.
    TreeFamiTF314083.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_03055. tRNA_methyltr_TrmB_euk.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q28H76-1 [UniParc]FASTAAdd to Basket

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    MCNTQATPSD PGITVAHPKK RYYRQRAHSN PMADHTFQYP VKPEIMDWSE    50
    YYPDYFKPLV PDSAHDDAKD LQERKEQHQV EFADIGCGYG GLLVELSPLF 100
    PNTLMLGLEI RVKVSDYVQD RIKSLRASHL GQYQNIACIR SNAMKYIPNF 150
    FKKGQLSKMF FLFPDPHFKK TKHKWRIISP TLLAEYAYVL RIGGMVYTIT 200
    DVEEVHTWMV KHFTEHPLFE RVAKEELVND IVVGKLGTST EEGKKVQRNK 250
    GLNLLAVFRR IENSTFIQRD SQQ 273
    Length:273
    Mass (Da):31,697
    Last modified:May 5, 2009 - v2
    Checksum:i79E31E012996D8D5
    GO

    Sequence cautioni

    The sequence AAH76665.1 differs from that shown. Reason: Erroneous initiation.
    The sequence CAJ83619.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti149 – 1491N → S in AAH76665. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR761007 mRNA. Translation: CAJ83619.1. Different initiation.
    BC076665 mRNA. Translation: AAH76665.1. Different initiation.
    RefSeqiNP_001006798.2. NM_001006797.2.
    UniGeneiStr.11051.

    Genome annotation databases

    EnsembliENSXETT00000021255; ENSXETP00000021255; ENSXETG00000009655.
    GeneIDi448504.
    KEGGixtr:448504.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR761007 mRNA. Translation: CAJ83619.1 . Different initiation.
    BC076665 mRNA. Translation: AAH76665.1 . Different initiation.
    RefSeqi NP_001006798.2. NM_001006797.2.
    UniGenei Str.11051.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 8364.ENSXETP00000021255.

    Proteomic databases

    PRIDEi Q28H76.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSXETT00000021255 ; ENSXETP00000021255 ; ENSXETG00000009655 .
    GeneIDi 448504.
    KEGGi xtr:448504.

    Organism-specific databases

    CTDi 4234.
    Xenbasei XB-GENE-1000662. mettl1.

    Phylogenomic databases

    eggNOGi COG0220.
    GeneTreei ENSGT00390000017840.
    HOGENOMi HOG000260965.
    InParanoidi Q6DFR8.
    KOi K03439.
    OrthoDBi EOG7PS1GC.
    TreeFami TF314083.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Gene expression databases

    Bgeei Q28H76.

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_03055. tRNA_methyltr_TrmB_euk.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR025763. Trm8_euk.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Sanger Xenopus tropicalis EST/cDNA project
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Egg.
    2. NIH - Xenopus Gene Collection (XGC) project
      Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Embryo.

    Entry informationi

    Entry nameiTRMBA_XENTR
    AccessioniPrimary (citable) accession number: Q28H76
    Secondary accession number(s): Q6DFR8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 5, 2009
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 60 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3