Q28H76 (TRMBA_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase A
EC=2.1.1.33

Alternative name(s):
Methyltransferase-like protein 1 A
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase A

Gene names

Name:	mettl1-A
ORF Names:	TEgg045g13.1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subunit structure	Forms a complex with wdr4 By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.
Sequence caution	The sequence AAH76665.1 differs from that shown. Reason: Erroneous initiation. The sequence CAJ83619.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from sequence or structural similarity. Source: UniProtKB tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	tRNA (guanine-N(7)-)-methyltransferase A		PRO_0000370560
Regions
	Region	109 – 110	2	S-adenosyl-L-methionine binding By similarity
	Region	142 – 143	2	S-adenosyl-L-methionine binding By similarity
	Region	240 – 242	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	165	1	By similarity
	Binding site	86	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	162	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Experimental info
	Sequence conflict	149	1	N → S in AAH76665. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q28H76 [UniParc]. Last modified May 5, 2009. Version 2. Checksum: 79E31E012996D8D5	FASTA	273	31,697
	10 20 30 40 50 60 MCNTQATPSD PGITVAHPKK RYYRQRAHSN PMADHTFQYP VKPEIMDWSE YYPDYFKPLV 70 80 90 100 110 120 PDSAHDDAKD LQERKEQHQV EFADIGCGYG GLLVELSPLF PNTLMLGLEI RVKVSDYVQD 130 140 150 160 170 180 RIKSLRASHL GQYQNIACIR SNAMKYIPNF FKKGQLSKMF FLFPDPHFKK TKHKWRIISP 190 200 210 220 230 240 TLLAEYAYVL RIGGMVYTIT DVEEVHTWMV KHFTEHPLFE RVAKEELVND IVVGKLGTST 250 260 270 EEGKKVQRNK GLNLLAVFRR IENSTFIQRD SQQ

References

[1]	Sanger Xenopus tropicalis EST/cDNA project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Egg.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR761007 mRNA. Translation: CAJ83619.1. Different initiation. BC076665 mRNA. Translation: AAH76665.1. Different initiation.
RefSeq	NP_001006798.2. NM_001006797.2.
UniGene	Str.11051.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	8364.ENSXETP00000021255.
Proteomic databases
PRIDE	Q28H76.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSXETT00000021255; ENSXETP00000021255; ENSXETG00000009655.
GeneID	448504.
KEGG	xtr:448504.
Organism-specific databases
CTD	4234.
Xenbase	XB-GENE-1000662. mettl1.
Phylogenomic databases
eggNOG	COG0220.
GeneTree	ENSGT00390000017840.
HOGENOM	HOG000260965.
InParanoid	Q6DFR8.
KO	K03439.
Enzyme and pathway databases
UniPathway	UPA00989.
Gene expression databases
Bgee	Q28H76.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. IPR025763. tRNA_(Gua-N-7)_MeTrfase_euk. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMBA_XENTR

Accession

Primary (citable) accession number: Q28H76
Secondary accession number(s): Q6DFR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	May 5, 2009
Last modified:	April 3, 2013
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program