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Q28H76 (TRMBA_XENTR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 58. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase A

EC=2.1.1.33
Alternative name(s):
Methyltransferase-like protein 1-A
tRNA (guanine(46)-N(7))-methyltransferase A
tRNA(m7G46)-methyltransferase A
Gene names
Name:mettl1-A
ORF Names:TEgg045g13.1
OrganismXenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]
Taxonomic identifier8364 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana

Protein attributes

Sequence length273 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subunit structure

Forms a complex with wdr4 By similarity. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Sequence caution

The sequence AAH76665.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAJ83619.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 273273tRNA (guanine-N(7)-)-methyltransferase A HAMAP-Rule MF_03055
PRO_0000370560

Regions

Region109 – 1102S-adenosyl-L-methionine binding By similarity
Region142 – 1432S-adenosyl-L-methionine binding By similarity
Region240 – 2423S-adenosyl-L-methionine binding By similarity

Sites

Active site1651 By similarity
Binding site861S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1621S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Experimental info

Sequence conflict1491N → S in AAH76665. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q28H76 [UniParc].

Last modified May 5, 2009. Version 2.
Checksum: 79E31E012996D8D5

FASTA27331,697
        10         20         30         40         50         60 
MCNTQATPSD PGITVAHPKK RYYRQRAHSN PMADHTFQYP VKPEIMDWSE YYPDYFKPLV 

        70         80         90        100        110        120 
PDSAHDDAKD LQERKEQHQV EFADIGCGYG GLLVELSPLF PNTLMLGLEI RVKVSDYVQD 

       130        140        150        160        170        180 
RIKSLRASHL GQYQNIACIR SNAMKYIPNF FKKGQLSKMF FLFPDPHFKK TKHKWRIISP 

       190        200        210        220        230        240 
TLLAEYAYVL RIGGMVYTIT DVEEVHTWMV KHFTEHPLFE RVAKEELVND IVVGKLGTST 

       250        260        270 
EEGKKVQRNK GLNLLAVFRR IENSTFIQRD SQQ 

« Hide

References

[1]Sanger Xenopus tropicalis EST/cDNA project
Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Egg.
[2]NIH - Xenopus Gene Collection (XGC) project
Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Embryo.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CR761007 mRNA. Translation: CAJ83619.1. Different initiation.
BC076665 mRNA. Translation: AAH76665.1. Different initiation.
RefSeqNP_001006798.2. NM_001006797.2.
UniGeneStr.11051.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING8364.ENSXETP00000021255.

Proteomic databases

PRIDEQ28H76.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSXETT00000021255; ENSXETP00000021255; ENSXETG00000009655.
GeneID448504.
KEGGxtr:448504.

Organism-specific databases

CTD4234.
XenbaseXB-GENE-1000662. mettl1.

Phylogenomic databases

eggNOGCOG0220.
GeneTreeENSGT00390000017840.
HOGENOMHOG000260965.
InParanoidQ6DFR8.
KOK03439.
OrthoDBEOG7PS1GC.
TreeFamTF314083.

Enzyme and pathway databases

UniPathwayUPA00989.

Gene expression databases

BgeeQ28H76.

Family and domain databases

HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMBA_XENTR
AccessionPrimary (citable) accession number: Q28H76
Secondary accession number(s): Q6DFR8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: April 16, 2014
This is version 58 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways