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Protein

tRNA (guanine-N(7)-)-methyltransferase A

Gene

mettl1-A

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the formation of N7-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N7-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei86S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei162S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei165UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase AUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
Methyltransferase-like protein 1-AUniRule annotation
tRNA (guanine(46)-N(7))-methyltransferase AUniRule annotation
tRNA(m7G46)-methyltransferase AUniRule annotation
Gene namesi
Name:mettl1-A
ORF Names:TEgg045g13.1
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
Proteomesi
  • UP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-1000662. mettl1.

Subcellular locationi

  • Nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705601 – 273tRNA (guanine-N(7)-)-methyltransferase AAdd BLAST273

Proteomic databases

PaxDbiQ28H76.
PRIDEiQ28H76.

Interactioni

Subunit structurei

Forms a complex with wdr4.UniRule annotation

Protein-protein interaction databases

STRINGi8364.ENSXETP00000021255.

Structurei

3D structure databases

SMRiQ28H76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni109 – 110S-adenosyl-L-methionine bindingUniRule annotation2
Regioni142 – 143S-adenosyl-L-methionine bindingUniRule annotation2
Regioni240 – 242S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115. Eukaryota.
COG0220. LUCA.
HOGENOMiHOG000260965.
InParanoidiQ28H76.
KOiK03439.
OrthoDBiEOG091G0IQ7.
TreeFamiTF314083.

Family and domain databases

HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PTHR23417:SF20. PTHR23417:SF20. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q28H76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCNTQATPSD PGITVAHPKK RYYRQRAHSN PMADHTFQYP VKPEIMDWSE
60 70 80 90 100
YYPDYFKPLV PDSAHDDAKD LQERKEQHQV EFADIGCGYG GLLVELSPLF
110 120 130 140 150
PNTLMLGLEI RVKVSDYVQD RIKSLRASHL GQYQNIACIR SNAMKYIPNF
160 170 180 190 200
FKKGQLSKMF FLFPDPHFKK TKHKWRIISP TLLAEYAYVL RIGGMVYTIT
210 220 230 240 250
DVEEVHTWMV KHFTEHPLFE RVAKEELVND IVVGKLGTST EEGKKVQRNK
260 270
GLNLLAVFRR IENSTFIQRD SQQ
Length:273
Mass (Da):31,697
Last modified:May 5, 2009 - v2
Checksum:i79E31E012996D8D5
GO

Sequence cautioni

The sequence AAH76665 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAJ83619 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti149N → S in AAH76665 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR761007 mRNA. Translation: CAJ83619.1. Different initiation.
BC076665 mRNA. Translation: AAH76665.1. Different initiation.
RefSeqiNP_001006798.2. NM_001006797.2.
UniGeneiStr.11051.

Genome annotation databases

GeneIDi448504.
KEGGixtr:448504.

Similar proteinsi

Entry informationi

Entry nameiTRMBA_XENTR
AccessioniPrimary (citable) accession number: Q28H76
Secondary accession number(s): Q6DFR8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: May 5, 2009
Last modified: August 30, 2017
This is version 73 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families