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Q28H12

- KCY_XENTR

UniProt

Q28H12 - KCY_XENTR

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Protein
UMP-CMP kinase
Gene
cmpk1, cmpk, TEgg001e20.1
Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the phosphorylation of pyrimidine nucleoside monophosphates at the expense of ATP. Plays an important role in de novo pyrimidine nucleotide biosynthesis. Has preference for UMP and CMP as phosphate acceptors. Also displays broad nucleoside diphosphate kinase activity By similarity.UniRule annotation

Catalytic activityi

ATP + (d)CMP = ADP + (d)CDP.UniRule annotation
ATP + UMP = ADP + UDP.UniRule annotation
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.UniRule annotation

Cofactori

Binds 1 magnesium ion per monomer By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei39 – 391NMP By similarity
Binding sitei100 – 1001CMP By similarity
Binding sitei134 – 1341ATP By similarity
Binding sitei140 – 1401NMP By similarity
Binding sitei151 – 1511NMP By similarity
Binding sitei179 – 1791ATP; via carbonyl oxygen By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 186ATP By similarity
Nucleotide bindingi61 – 633NMP By similarity
Nucleotide bindingi93 – 964NMP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cytidylate kinase activity Source: UniProtKB-EC
  3. nucleoside diphosphate kinase activity Source: UniProtKB

GO - Biological processi

  1. nucleoside diphosphate phosphorylation Source: UniProtKB
  2. nucleoside triphosphate biosynthetic process Source: UniProtKB
  3. pyrimidine nucleotide biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Pyrimidine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
UMP-CMP kinase (EC:2.7.4.14)
Alternative name(s):
Deoxycytidylate kinase
Short name:
CK
Short name:
dCMP kinase
Nucleoside-diphosphate kinase (EC:2.7.4.6)
Uridine monophosphate/cytidine monophosphate kinase
Short name:
UMP/CMP kinase
Short name:
UMP/CMPK
Gene namesi
Name:cmpk1
Synonyms:cmpk
ORF Names:TEgg001e20.1
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143: Unplaced

Organism-specific databases

XenbaseiXB-GENE-980040. cmpk1.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 196196UMP-CMP kinaseUniRule annotation
PRO_0000292027Add
BLAST

Proteomic databases

PaxDbiQ28H12.

Expressioni

Gene expression databases

BgeeiQ28H12.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi8364.ENSXETP00000035667.

Structurei

3D structure databases

ProteinModelPortaliQ28H12.
SMRiQ28H12. Positions 3-196.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni33 – 6331NMPbind By similarity
Add
BLAST
Regioni133 – 14311LID By similarity
Add
BLAST

Domaini

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0563.
GeneTreeiENSGT00390000016215.
HOGENOMiHOG000238771.
HOVERGENiHBG108060.
OrthoDBiEOG7X0VJ0.
TreeFamiTF354283.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00235. Adenylate_kinase_Adk.
MF_03172. Adenylate_kinase_UMP_CMP_kin.
InterProiIPR000850. Adenylat/UMP-CMP_kin.
IPR027417. P-loop_NTPase.
IPR006266. UMP_CMP_kinase.
[Graphical view]
PANTHERiPTHR23359. PTHR23359. 1 hit.
PRINTSiPR00094. ADENYLTKNASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR01359. UMP_CMP_kin_fam. 1 hit.
PROSITEiPS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q28H12-1 [UniParc]FASTAAdd to Basket

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MKPFVVFVLG GPGAGKGTQC ERIVQKYGYT HLSAGDLLRD ERKKPDSQYG    50
ELIESYIRDG RIVPVEITIS LLQRAMEQTM ALDGNKHKFL IDGFPRNEDN 100
LQGWERTMNG KADVSFVLFF DCDNETCIER CLERGKSSGR SDDNRESLEK 150
RIQTYLQSTR PIIDLYEKTG KVKKVDASKS VDEVFTKVQD IFDREG 196
Length:196
Mass (Da):22,352
Last modified:June 26, 2007 - v2
Checksum:i1E1B1F9E771F8B7A
GO

Sequence cautioni

The sequence CAJ82118.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR761111 mRNA. Translation: CAJ82118.1. Different initiation.
UniGeneiStr.22202.

Genome annotation databases

EnsembliENSXETT00000035667; ENSXETP00000035667; ENSXETG00000016340.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR761111 mRNA. Translation: CAJ82118.1 . Different initiation.
UniGenei Str.22202.

3D structure databases

ProteinModelPortali Q28H12.
SMRi Q28H12. Positions 3-196.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 8364.ENSXETP00000035667.

Proteomic databases

PaxDbi Q28H12.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSXETT00000035667 ; ENSXETP00000035667 ; ENSXETG00000016340 .

Organism-specific databases

Xenbasei XB-GENE-980040. cmpk1.

Phylogenomic databases

eggNOGi COG0563.
GeneTreei ENSGT00390000016215.
HOGENOMi HOG000238771.
HOVERGENi HBG108060.
OrthoDBi EOG7X0VJ0.
TreeFami TF354283.

Gene expression databases

Bgeei Q28H12.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
HAMAPi MF_00235. Adenylate_kinase_Adk.
MF_03172. Adenylate_kinase_UMP_CMP_kin.
InterProi IPR000850. Adenylat/UMP-CMP_kin.
IPR027417. P-loop_NTPase.
IPR006266. UMP_CMP_kinase.
[Graphical view ]
PANTHERi PTHR23359. PTHR23359. 1 hit.
PRINTSi PR00094. ADENYLTKNASE.
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR01359. UMP_CMP_kin_fam. 1 hit.
PROSITEi PS00113. ADENYLATE_KINASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Sanger Xenopus tropicalis EST/cDNA project
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Egg.

Entry informationi

Entry nameiKCY_XENTR
AccessioniPrimary (citable) accession number: Q28H12
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 26, 2007
Last modified: April 16, 2014
This is version 52 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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