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Protein

Maternal embryonic leucine zipper kinase

Gene

melk

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, self-renewal of stem cells, apoptosis and splicing regulation. Also plays a role in primitive hematopoiesis, possibly by affecting the expression of genes critical for hematopoiesis. Plays a role in cytokinesis during early development (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Activated by autophosphorylation of the T-loop at Thr-169 and Ser-173: in contrast to other members of the SNF1 subfamily, phosphorylation at Thr-169 is not mediated by STK11/LKB1 but via autophosphorylation instead.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei42ATPPROSITE-ProRule annotation1
Active sitei134Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi19 – 27ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis, Cell cycle

Keywords - Ligandi

ATP-binding, Lipid-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Maternal embryonic leucine zipper kinase (EC:2.7.11.1)
Gene namesi
Name:melk
ORF Names:TEgg058f04.1
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
Proteomesi
  • UP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-987654. melk.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004134311 – 652Maternal embryonic leucine zipper kinaseAdd BLAST652

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei169Phosphothreonine; by autocatalysisBy similarity1
Modified residuei173Phosphoserine; by autocatalysisBy similarity1
Modified residuei415PhosphothreonineBy similarity1
Modified residuei450PhosphothreonineBy similarity1
Modified residuei452PhosphothreonineBy similarity1
Modified residuei482PhosphothreonineBy similarity1
Modified residuei484PhosphothreonineBy similarity1
Modified residuei499PhosphoserineBy similarity1
Modified residuei506PhosphoserineBy similarity1
Modified residuei518PhosphoserineBy similarity1

Post-translational modificationi

Autophosphorylated: autophosphorylation of the T-loop at Thr-169 and Ser-173 is required for activation. Phosphorylated by the maturation promoting factor (MPF), composed of cdk1 and a cyclin-B. Also phosphorylated by some MAPK. Phosphorylated during oocyte maturation. Dephosphorylation destabilizes the protein (By similarity).By similarity
Degraded when cells exit mitosis.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ28GW8.

Interactioni

Protein-protein interaction databases

STRINGi8364.ENSXETP00000014336.

Structurei

3D structure databases

ProteinModelPortaliQ28GW8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini13 – 265Protein kinasePROSITE-ProRule annotationAdd BLAST253
Domaini603 – 652KA1PROSITE-ProRule annotationAdd BLAST50

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni284 – 323UBA-likeBy similarityAdd BLAST40
Regioni328 – 652Autoinhibitory regionBy similarityAdd BLAST325

Domaini

The KA1 domain mediates binding to phospholipids and targeting to membranes.By similarity

Sequence similaritiesi

Contains 1 KA1 (kinase-associated) domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233023.
HOVERGENiHBG106273.
InParanoidiQ28GW8.
KOiK08799.

Family and domain databases

Gene3Di3.30.310.80. 1 hit.
InterProiIPR028375. KA1/Ssp2_C.
IPR001772. KA1_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF02149. KA1. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF103243. SSF103243. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50032. KA1. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q28GW8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVDDYEELL KYYELHETIG TGGFAKVKLA SHLTTGEKVA IKIMDKESLG
60 70 80 90 100
DDLPRVKTEI DAMKNLSHQH VCRLYHVIET PNKIFMVLEY CPGGELFDYI
110 120 130 140 150
IAKDRLTEDE ARVFFRQIVS AVAYIHSQGY AHRDLKPENL LIDEDQNLKL
160 170 180 190 200
IDFGLCAKPK GGLDYHLMTC CGSPAYAAPE LIQGKAYIGS EADIWSMGVL
210 220 230 240 250
MYALMCGYLP FDDDNVMVLY KKIMRGKYEI PKWLSPGSVL LLSQMLQVDP
260 270 280 290 300
KKRISVKHLL SHPWLMQGYS CPVEWQSKYP LGYVDEDCVT ELSVFYKCSR
310 320 330 340 350
TSTSRLISEW NYDHITASYL LLHSKKSHGK PVRLKRPLAV GDQPVTSFKE
360 370 380 390 400
LRPKSTLDFE EPNCGEIAYV FGSMEFSDDE LFSEDFAYSC FEPHTPKEYV
410 420 430 440 450
KGRSEFHSVD SAPSTPVVQR YARHKSEDKE NCDAALGKDE NVFLHPAPWT
460 470 480 490 500
PTPRRKQNEK KGILTTPNKN SHTKEKNQSK ETPTKKPITT GEELANVISP
510 520 530 540 550
ERRCRSVELD LNQAHVDSAQ KKKGAKVFGS LERGLDKMIT MLTPSKRKGY
560 570 580 590 600
AREGPRKLRA HYNVTTTNIM NPEQLLNQIV KVLPSKNVDY VQKGYTLKCK
610 620 630 640 650
TQSDFGKVTM QFELEVCQLS KSEMVGIRRQ RLKGDAWVYK RLVEDILSSC

KV
Length:652
Mass (Da):74,276
Last modified:April 4, 2006 - v1
Checksum:i81BEBB0F76999EB3
GO

Sequence cautioni

The sequence AAI35246 differs from that shown. Reason: Erroneous termination at position 508. Translated as Glu.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR761185 mRNA. Translation: CAJ81864.1.
AAMC01051129 Genomic DNA. No translation available.
AAMC01051130 Genomic DNA. No translation available.
AAMC01051131 Genomic DNA. No translation available.
BC135245 mRNA. Translation: AAI35246.1. Sequence problems.
RefSeqiNP_001016390.1. NM_001016390.2.
UniGeneiStr.7658.

Genome annotation databases

GeneIDi549144.
KEGGixtr:549144.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR761185 mRNA. Translation: CAJ81864.1.
AAMC01051129 Genomic DNA. No translation available.
AAMC01051130 Genomic DNA. No translation available.
AAMC01051131 Genomic DNA. No translation available.
BC135245 mRNA. Translation: AAI35246.1. Sequence problems.
RefSeqiNP_001016390.1. NM_001016390.2.
UniGeneiStr.7658.

3D structure databases

ProteinModelPortaliQ28GW8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi8364.ENSXETP00000014336.

Proteomic databases

PaxDbiQ28GW8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi549144.
KEGGixtr:549144.

Organism-specific databases

CTDi9833.
XenbaseiXB-GENE-987654. melk.

Phylogenomic databases

eggNOGiKOG0583. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000233023.
HOVERGENiHBG106273.
InParanoidiQ28GW8.
KOiK08799.

Family and domain databases

Gene3Di3.30.310.80. 1 hit.
InterProiIPR028375. KA1/Ssp2_C.
IPR001772. KA1_dom.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF02149. KA1. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF103243. SSF103243. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50032. KA1. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMELK_XENTR
AccessioniPrimary (citable) accession number: Q28GW8
Secondary accession number(s): A4IGU0, F7BW48, F7BW55
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 19, 2011
Last sequence update: April 4, 2006
Last modified: November 30, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.