Q28GG8 (TBP_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
TATA-box-binding protein

Alternative name(s):
TATA sequence-binding protein
TATA-binding factor
TATA-box factor
Transcription initiation factor TFIID TBP subunit

Gene names

Name:	tbp
Synonyms:	tfiid
ORF Names:	TEgg042d23.1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	297 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Members of the TBP family are differentially required to regulate transcription and development during early embryogenesis By similarity. Binds to the promoters of select genes. Ref.3 UniProtKB P27633
Subunit structure	Belongs to the TFIID complex together with the TBP-associated factors (TAFs). Binds DNA as monomer.
Subcellular location	Nucleus.
Post-translational modification	The N-terminal domain is extensively phosphorylated By similarity. UniProtKB P27633
Sequence similarities	Belongs to the TBP family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat
Ligand	DNA-binding
Molecular function	Developmental protein
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA-dependent transcription, initiation Inferred from electronic annotation. Source: InterPro embryo development Inferred from sequence or structural similarity. Source: UniProtKB gastrulation Inferred from electronic annotation. Source: Compara mRNA catabolic process Inferred from electronic annotation. Source: Compara regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	DNA binding Inferred from direct assay Ref.3. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 297	297	TATA-box-binding protein		PRO_0000348609
Regions
	Repeat	123 – 199	77	1
	Repeat	213 – 290	78	2
	Compositional bias	54 – 83	30	Gln-rich

Sequences

	Sequence		Length	Mass (Da)
	Q28GG8 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 0CBF63B82EEA1A9A	FASTA	297	32,693
	10 20 30 40 50 60 MDQNNSIPPF QGLASPQGSL TPGINIFSPL MPYGTGLTPQ PVQTTNSLSI LEEQQRQQQQ 70 80 90 100 110 120 AQQSTSQQGN QGSGQTPQLF HPQTLTTAPL PGNTPLYPSP MTPMTPITPA TPASESSGIV 130 140 150 160 170 180 PQLQNIVSTV NLGCKLDLKT IALRARNAEY NPKRFAAVIM RIREPRTTAL IFSSGKMVCT 190 200 210 220 230 240 GAKSEEQSRL AARKYARVVQ KLGFPAKFLD FKIQNMVGSC DVKFPIRLEG LVLTHQQFSS 250 260 270 280 290 YEPELFPGLI YRMIKPRIVL LIFVSGKVVL TGAKVRAEIY EAFENIYPIL KGFRKTT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Sanger Xenopus tropicalis EST/cDNA project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Egg.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.
[3]	"TBP paralogs accommodate metazoan- and vertebrate-specific developmental gene regulation." Jacobi U.G., Akkers R.C., Pierson E.S., Weeks D.L., Dagle J.M., Veenstra G.J.C. EMBO J. 26:3900-3909(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR761393 mRNA. Translation: CAJ83650.1. BC160510 mRNA. Translation: AAI60510.1.
RefSeq	NP_001016339.1. NM_001016339.3.
UniGene	Str.6374.
3D structure databases
ProteinModelPortal	Q28GG8.
SMR	Q28GG8. Positions 117-296.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	549093.
KEGG	xtr:549093.
Organism-specific databases
CTD	6908.
Xenbase	XB-GENE-488470. tbp.
Phylogenomic databases
eggNOG	COG2101.
HOGENOM	HOG000105164.
HOVERGEN	HBG044997.
KO	K03120.
OrthoDB	EOG4PVP0C.
Gene expression databases
Bgee	Q28GG8.
Family and domain databases
Gene3D	3.30.310.10. 2 hits.
InterPro	IPR012295. Beta2_adaptin/TBP_C_dom. IPR000814. TBP. [Graphical view]
PANTHER	PTHR10126. PTHR10126. 1 hit.
Pfam	PF00352. TBP. 2 hits. [Graphical view]
PRINTS	PR00686. TIFACTORIID.
PROSITE	PS00351. TFIID. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TBP_XENTR

Accession

Primary (citable) accession number: Q28GG8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	April 4, 2006
Last modified:	May 29, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program