Q28FC1 (UBE2W_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 W
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein W
Ubiquitin-protein ligase W

Gene names

Name:	ube2w
ORF Names:	TEgg096e02.1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in degradation of misfolded chaperone substrate and DNA repair By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	DNA damage DNA repair Ubl conjugation pathway
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW cellular response to misfolded protein Inferred from sequence or structural similarity. Source: UniProtKB misfolded or incompletely synthesized protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB proteasomal ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB protein monoubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 151	151	Ubiquitin-conjugating enzyme E2 W		PRO_0000416882
Sites
	Active site	91	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28FC1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: B91B585BBD091F34	FASTA	151	17,331
	10 20 30 40 50 60 MASMQKRLQK ELLALQNEPP PGMTLNEKSV QNSITQWIVD MEGAPGTLYE GEKFQLLFKF 70 80 90 100 110 120 SSRYPFDSPQ VMFTGDNIPV HPHVYSNGHI CLSILTEDWS PALSVQSVCL SIISMLSSCK 130 140 150 EKRRPPDNSF YVRTCNKNPK KTKWWYHDDT C

References

[1]	Sanger Xenopus tropicalis EST/cDNA project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Egg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR762043 mRNA. Translation: CAJ83690.1.
RefSeq	NP_001016132.1. NM_001016132.2.
UniGene	Str.19999.
3D structure databases
ProteinModelPortal	Q28FC1.
SMR	Q28FC1. Positions 1-117.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	548886.
KEGG	xtr:548886.
Organism-specific databases
CTD	55284.
Xenbase	XB-GENE-954746. ube2w.
Phylogenomic databases
HOVERGEN	HBG063308.
KO	K10688.
Enzyme and pathway databases
UniPathway	UPA00143.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBE2W_XENTR

Accession

Primary (citable) accession number: Q28FC1

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 18, 2012
Last sequence update:	April 4, 2006
Last modified:	March 6, 2013
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program