Q28F89 (UBE2S_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 S
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein S
Ubiquitin-protein ligase S

Gene names

Name:	ube2s
ORF Names:	TEgg003m12.1, TEgg028l19.1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme ube2c/ubch10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	activation of anaphase-promoting complex activity Inferred from sequence or structural similarity. Source: UniProtKB anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process Inferred from sequence or structural similarity. Source: UniProtKB cell division Inferred from electronic annotation. Source: UniProtKB-KW exit from mitosis Inferred from sequence or structural similarity. Source: UniProtKB free ubiquitin chain polymerization Inferred from sequence or structural similarity. Source: UniProtKB protein K11-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	anaphase-promoting complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 211	211	Ubiquitin-conjugating enzyme E2 S		PRO_0000390434
Sites
	Active site	95	1	Glycyl thioester intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28F89 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 49818D7A14991D62	FASTA	211	23,288
	10 20 30 40 50 60 MNSNVENLPP HIIRQVYKEV STLTSDPPEG IKIIPNEEDI TDVQVNIEGP EGTPYAGGIF 70 80 90 100 110 120 RMKLILGKDF PAAPPKGYFL TKIFHPNVST NGEICVNVLK KDWKAELGIR HVLLTIKCLL 130 140 150 160 170 180 IHPNPESALN EEAGRLLLEN YEEYASRARL MTEIHAQGSS LRGKDPTDPC SSASATLVSG 190 200 210 DGPMAKKHAG DRDKKLAAKK KTDKKRALRR L

References

[1]	Sanger Xenopus tropicalis EST/cDNA project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Egg.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Neurula.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR761031 mRNA. Translation: CAJ83662.1. CR761379 mRNA. Translation: CAJ82166.1. CR762108 mRNA. Translation: CAJ81686.1. BC170651 mRNA. Translation: AAI70651.1. BC170653 mRNA. Translation: AAI70653.1.
RefSeq	NP_001016438.1. NM_001016438.2.
UniGene	Str.6451.
3D structure databases
ProteinModelPortal	Q28F89.
SMR	Q28F89. Positions 6-156.
ModBase	Search...
Protein-protein interaction databases
STRING	8364.ENSXETP00000000266.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSXETT00000064784; ENSXETP00000063683; ENSXETG00000000138.
GeneID	549192.
KEGG	xtr:549192.
Organism-specific databases
CTD	27338.
Xenbase	XB-GENE-1008017. ube2s.
Phylogenomic databases
eggNOG	COG5078.
GeneTree	ENSGT00680000099547.
HOGENOM	HOG000233455.
HOVERGEN	HBG063308.
KO	K10583.
OMA	QICADIE.
Enzyme and pathway databases
UniPathway	UPA00143.
Gene expression databases
Bgee	Q28F89.
Family and domain databases
Gene3D	3.10.110.10. 1 hit.
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBE2S_XENTR

Accession

Primary (citable) accession number: Q28F89
Secondary accession number(s): Q28GI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2009
Last sequence update:	April 4, 2006
Last modified:	April 3, 2013
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program