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Protein

Ubiquitin-conjugating enzyme E2 S

Gene

ube2s

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme ube2c/ubch10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit (By similarity).PROSITE-ProRule annotation

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathway:iprotein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei95 – 951Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Cell cycle, Cell division, Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 S (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein S
Ubiquitin-protein ligase S
Gene namesi
Name:ube2s
ORF Names:TEgg003m12.1, TEgg028l19.1
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-1008017. ube2s.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 211211Ubiquitin-conjugating enzyme E2 SPRO_0000390434Add
BLAST

Expressioni

Gene expression databases

BgeeiQ28F89.

Interactioni

Protein-protein interaction databases

STRINGi8364.ENSXETP00000063683.

Structurei

3D structure databases

ProteinModelPortaliQ28F89.
SMRiQ28F89. Positions 6-156.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00730000110565.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiQ28F89.
KOiK10583.
OMAiKKDWKPD.
OrthoDBiEOG73JKXD.
TreeFamiTF101120.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q28F89-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSNVENLPP HIIRQVYKEV STLTSDPPEG IKIIPNEEDI TDVQVNIEGP
60 70 80 90 100
EGTPYAGGIF RMKLILGKDF PAAPPKGYFL TKIFHPNVST NGEICVNVLK
110 120 130 140 150
KDWKAELGIR HVLLTIKCLL IHPNPESALN EEAGRLLLEN YEEYASRARL
160 170 180 190 200
MTEIHAQGSS LRGKDPTDPC SSASATLVSG DGPMAKKHAG DRDKKLAAKK
210
KTDKKRALRR L
Length:211
Mass (Da):23,288
Last modified:April 4, 2006 - v1
Checksum:i49818D7A14991D62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR761031 mRNA. Translation: CAJ83662.1.
CR761379 mRNA. Translation: CAJ82166.1.
CR762108 mRNA. Translation: CAJ81686.1.
BC170651 mRNA. Translation: AAI70651.1.
BC170653 mRNA. Translation: AAI70653.1.
RefSeqiNP_001016438.1. NM_001016438.2.
UniGeneiStr.6451.

Genome annotation databases

EnsembliENSXETT00000064784; ENSXETP00000063683; ENSXETG00000000138.
GeneIDi549192.
KEGGixtr:549192.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR761031 mRNA. Translation: CAJ83662.1.
CR761379 mRNA. Translation: CAJ82166.1.
CR762108 mRNA. Translation: CAJ81686.1.
BC170651 mRNA. Translation: AAI70651.1.
BC170653 mRNA. Translation: AAI70653.1.
RefSeqiNP_001016438.1. NM_001016438.2.
UniGeneiStr.6451.

3D structure databases

ProteinModelPortaliQ28F89.
SMRiQ28F89. Positions 6-156.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi8364.ENSXETP00000063683.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSXETT00000064784; ENSXETP00000063683; ENSXETG00000000138.
GeneIDi549192.
KEGGixtr:549192.

Organism-specific databases

CTDi27338.
XenbaseiXB-GENE-1008017. ube2s.

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00730000110565.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiQ28F89.
KOiK10583.
OMAiKKDWKPD.
OrthoDBiEOG73JKXD.
TreeFamiTF101120.

Enzyme and pathway databases

UniPathwayiUPA00143.

Gene expression databases

BgeeiQ28F89.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Sanger Xenopus tropicalis EST/cDNA project
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Egg.
  2. NIH - Xenopus Gene Collection (XGC) project
    Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Neurula.

Entry informationi

Entry nameiUBE2S_XENTR
AccessioniPrimary (citable) accession number: Q28F89
Secondary accession number(s): Q28GI1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: April 4, 2006
Last modified: July 22, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.