Reviewed, UniProtKB/Swiss-Prot Q28ED0 (DHSA_XENTR)

Names and origin

Protein names

Recommended name:
    Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial
    EC=1.3.5.1
Alternative name(s):
    Flavoprotein subunit of complex II
      Short name=Fp

Gene names

Name:	sdha
ORF Names:	TNeu107a09.1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	665 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Flavoprotein (FP) subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q) By similarity.
Catalytic activity	Succinate + ubiquinone = fumarate + ubiquinol.
Cofactor	FAD By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Subunit structure	Component of complex II composed of four subunits: the flavoprotein (FP) sdha, iron-sulfur protein (IP) sdhb, and a cytochrome b560 composed of sdhc and sdhd By similarity.
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix sideBy similarity.
Sequence similarities	Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro succinate dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 43	43	Mitochondrion Potential
	Chain	44 – 665	622	Succinate dehydrogenase [ubiquinone] flavoprotein subunit, mitochondrial		PRO_0000272307
Regions
	Nucleotide binding	94 – 109	16	FAD By similarity
Sites
	Active site	343	1	Proton acceptor By similarity
	Binding site	299	1	Substrate By similarity
	Binding site	311	1	Substrate By similarity
	Binding site	410	1	Substrate By similarity
	Binding site	454	1	Substrate By similarity
Amino acid modifications
	Modified residue	102	1	Tele-8alpha-FAD histidine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28ED0-1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 6C0A8263F91E0649	FASTA	665	72,706
	10 20 30 40 50 60 MAFLNVASSR LLSKTLQLGG RVQNCTATCT AATRNFHFSV YGRKDTSAKL SDSISTQYPV 70 80 90 100 110 120 VDHDFDAVVV GAGGAGLRAA FGLSEAGFNT ACITKLFPTR SHTVAAQGGI NAALGNMEDD 130 140 150 160 170 180 DWRWHFYDTV KGSDWLGDQD AIHYMTEQAP ASVIELENYG MPFSRTEQGK IYQRAFGGQS 190 200 210 220 230 240 LKFGKGGQAH RCCCVADRTG HSLLHTLYGR SLRYDTSYFV EYFALDLLME NGECRGVIAL 250 260 270 280 290 300 CMEDGSIHRF RAKNTVIATG GYGRTFFSCT SAHTCTGDGT AMVTRAGLPC QDLEFVQFHP 310 320 330 340 350 360 TGIYGAGCLI TEGCRGEGGI LINSEGERFM ERYAPVAKDL ASRDVVSRSM TIEIREGRGC 370 380 390 400 410 420 GKDKDHVYLQ LHHLPPSQLA SRLPGISETA MIFAGVDVTK EPIPVLPTVH YNMGGIPTNY 430 440 450 460 470 480 KGQVITHVNG EDRVVPGLYA CGEAASASVH GANRLGANSL LDLVVFGRAC ALSIAESCKP 490 500 510 520 530 540 GEPVPSIKEN AGEESVANLD KLRFTNGSTR TSELRINMQK TMQNHAAVFR TGSVLKEGCE 550 560 570 580 590 600 KLSAINSTMD DIKTFDRGIV WNTDLVETLE LQNLMLCALQ TIYGAEARKE SRGAHAREDY 610 620 630 640 650 660 KVRIDEYDYS KPIQGQQKKS FSEHWRKHTL SYVDGKGKVS LEYRPVIDTT LNEDCVSVPP AIRSY

References

[1]	Sanger Xenopus tropicalis EST/cDNA project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Neurula.
[2]	NIH - Xenopus Gene Collection (XGC) project Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Embryo.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CR848322 mRNA. Translation: CAJ83256.1. BC135730 mRNA. Translation: AAI35731.1.
RefSeq	NP_001015989.1.
UniGene	Str.9006
3D structure databases
SMR	Q28ED0. Positions 56-665.
ModBase	Search...
Genome annotation databases
GeneID	548743.
KEGG	xtr:548743.
Organism-specific databases
Xenbase	XB-FEAT-956941. sdha.
Phylogenomic databases
HOVERGEN	Q28ED0.
Family and domain databases
InterPro	IPR003953. FAD_bind2_N. IPR013027. FAD_pyr_nucl-diS_OxRdtase. IPR003952. FRD_SDH_FAD_BS. IPR004112. Fum_Rdtase/Succ_DHase_flav_C. IPR011281. Succ_DHase_flav_su_fwd. IPR014006. Succ_Dhase_frdA_Gneg. [Graphical view]
Pfam	PF00890. FAD_binding_2. 1 hit. PF02910. Succ_DH_flav_C. 1 hit. [Graphical view]
PRINTS	PR00368. FADPNR.
TIGRFAMs	TIGR01816. sdhA_forward. 1 hit. TIGR01812. sdhA_frdA_Gneg. 1 hit.
PROSITE	PS00504. FRD_SDH_FAD_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHSA_XENTR

Accession

Primary (citable) accession number: Q28ED0
Secondary accession number(s): A4QNI5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2007
Last sequence update:	April 4, 2006
Last modified:	November 25, 2008
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project