Reviewed, UniProtKB/Swiss-Prot Q28E61 (NSUN2_XENTR)

Names and origin

Protein names

Recommended name:
    tRNA (cytosine-5-)-methyltransferase NSUN2
    EC=2.1.1.29
Alternative name(s):
    NOL1/NOP2/Sun domain family member 2

Gene names

Name:	nsun2
ORF Names:	TEgg048n02.1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	798 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing 5-methylcytosine.
Subcellular location	Nucleus › nucleolus By similarity. Cytoplasm By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	tRNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 798	798	tRNA (cytosine-5-)-methyltransferase NSUN2		PRO_0000289227
Regions
	Region	186 – 192	7	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	323	1	Nucleophile Potential
	Binding site	217	1	S-adenosyl-L-methionine By similarity
	Binding site	244	1	S-adenosyl-L-methionine By similarity
	Binding site	270	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28E61-1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 3F79B94FBDED8AA8	FASTA	798	91,020
	10 20 30 40 50 60 MGRRNRRNRQ RHQRSTEQRS PAEEEQRRKA REQAAWECGY PEIIKENKLF EHYYQELKIV 70 80 90 100 110 120 PDGEWDKFMA SLREPLPATI RITGYKSHAK EILHCLKEKY FKELQDIEVD GQKIEAPQPL 130 140 150 160 170 180 SWYPEELAWH TNLSRKIIRK SPELEKFHQF LVNETESGNI SRQEAVSMIP PVLLKVQPHH 190 200 210 220 230 240 KILDMCAAPG SKTAQIIEML HADMNVPFPE GFVIANDVDN KRCYLLVHQA KRLNSPCIMV 250 260 270 280 290 300 VNHDASSIPR LLVENNGSRE VLYYDRILCD VPCSGDGTLR KNIDVWKKWT TLNSLQLHGL 310 320 330 340 350 360 QIRIATRGVE QLAEGGRMVY STCSLNPVED EAVIASLLDK SEGSLELADV ASEIPGLKWM 370 380 390 400 410 420 PGITQWKVMT KEGHWYEKWE DIPTSRHTQI RPTMFPPKDE EKLKSMNLNR CMRILPHHQN 430 440 450 460 470 480 TGGFFVAVLI KKAPMPWNKR QPKLQRRPPV SACDASIAVA PELVKAVTEN SAGMADEPAV 490 500 510 520 530 540 DTENGETKPC TNQSDSSKTD IVCCPPPSKK MKLFGFKEDP FVFVSEDDPI FDPIQTFYAL 550 560 570 580 590 600 DPSFPKKNLL TRTQEGKKRQ LYMVSKELRN VLLHNSEKMK VINTGIKVLC RNNDGEQYGC 610 620 630 640 650 660 AYRLAQEGIY TLYPFINARI VTVSIEDIKV LLTQENPFLS KFSKETQKQA NNFDMGSIVL 670 680 690 700 710 720 KYEPDPQEPE TLQCPIVLCG WRGKTSIRSF VPKNERLHYL RMMGVEVFKE KAEVLEKKPV 730 740 750 760 770 780 EGKACDEEHI DEKMDIDGAK EESKELSGNE SGDDEDPKEE DVIDRGVLEH VALKNTSAIP 790 ASVEDQAEDA SVSKESVD

References

[1]	Sanger Xenopus tropicalis EST/cDNA project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Egg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR848434 mRNA. Translation: CAJ83692.1.
RefSeq	NP_001015962.1.
UniGene	Str.20500
3D structure databases
SMR	Q28E61. Positions 49-635.
ModBase	Search...
Protein-protein interaction databases
STRING	Q28E61.
Genome annotation databases
GeneID	548716.
KEGG	xtr:548716.
Organism-specific databases
CTD	548716.
Xenbase	XB-FEAT-962622. nsun2.
Phylogenomic databases
eggNOG	veNOG09414.
HOVERGEN	Q28E61.
Enzyme and pathway databases
BRENDA	2.1.1.29. 279072.
Family and domain databases
InterPro	IPR001678. Fmu/NOL1/Nop2p. [Graphical view]
Pfam	PF01189. Nol1_Nop2_Fmu. 1 hit. [Graphical view]
PROSITE	PS01153. NOL1_NOP2_SUN. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NSUN2_XENTR

Accession

Primary (citable) accession number: Q28E61

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 29, 2007
Last sequence update:	April 4, 2006
Last modified:	January 19, 2010
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Xenopus annotation project