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Protein

tRNA (cytosine(34)-C(5))-methyltransferase

Gene

nsun2

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5-methylcytosine (m5C) at positions 34 and 48 of intron-containing tRNA(Leu)(CAA) precursors, and at positions 48, 49 and 50 of tRNA(Gly)(GCC) precursors (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + cytosine(34) in tRNA precursor = S-adenosyl-L-homocysteine + 5-methylcytosine(34) in tRNA precursor.

Enzyme regulationi

Inhibited by magnesium ions.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei217 – 2171S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei244 – 2441S-adenosyl-L-methioninePROSITE-ProRule annotation
Binding sitei270 – 2701S-adenosyl-L-methioninePROSITE-ProRule annotation
Active sitei323 – 3231NucleophilePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytosine(34)-C(5))-methyltransferase (EC:2.1.1.203)
Alternative name(s):
NOL1/NOP2/Sun domain family member 2
Gene namesi
Name:nsun2
ORF Names:TEgg048n02.1
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
Proteomesi
  • UP000008143 Componenti: Unassembled WGS sequence

Organism-specific databases

XenbaseiXB-GENE-962623. nsun2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 798798tRNA (cytosine(34)-C(5))-methyltransferasePRO_0000289227Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ28E61.

Interactioni

Protein-protein interaction databases

STRINGi8364.ENSXETP00000059424.

Structurei

3D structure databases

ProteinModelPortaliQ28E61.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni186 – 1927S-adenosyl-L-methionine bindingPROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family. TRM4 subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2198. Eukaryota.
COG0144. LUCA.
HOGENOMiHOG000205147.
HOVERGENiHBG106711.
InParanoidiQ28E61.
KOiK15335.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR001678. MeTrfase_RsmB/NOP2.
IPR023267. RCMT.
IPR023270. RCMT_NCL1.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
PR02011. RCMTNCL1.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q28E61-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRRNRRNRQ RHQRSTEQRS PAEEEQRRKA REQAAWECGY PEIIKENKLF
60 70 80 90 100
EHYYQELKIV PDGEWDKFMA SLREPLPATI RITGYKSHAK EILHCLKEKY
110 120 130 140 150
FKELQDIEVD GQKIEAPQPL SWYPEELAWH TNLSRKIIRK SPELEKFHQF
160 170 180 190 200
LVNETESGNI SRQEAVSMIP PVLLKVQPHH KILDMCAAPG SKTAQIIEML
210 220 230 240 250
HADMNVPFPE GFVIANDVDN KRCYLLVHQA KRLNSPCIMV VNHDASSIPR
260 270 280 290 300
LLVENNGSRE VLYYDRILCD VPCSGDGTLR KNIDVWKKWT TLNSLQLHGL
310 320 330 340 350
QIRIATRGVE QLAEGGRMVY STCSLNPVED EAVIASLLDK SEGSLELADV
360 370 380 390 400
ASEIPGLKWM PGITQWKVMT KEGHWYEKWE DIPTSRHTQI RPTMFPPKDE
410 420 430 440 450
EKLKSMNLNR CMRILPHHQN TGGFFVAVLI KKAPMPWNKR QPKLQRRPPV
460 470 480 490 500
SACDASIAVA PELVKAVTEN SAGMADEPAV DTENGETKPC TNQSDSSKTD
510 520 530 540 550
IVCCPPPSKK MKLFGFKEDP FVFVSEDDPI FDPIQTFYAL DPSFPKKNLL
560 570 580 590 600
TRTQEGKKRQ LYMVSKELRN VLLHNSEKMK VINTGIKVLC RNNDGEQYGC
610 620 630 640 650
AYRLAQEGIY TLYPFINARI VTVSIEDIKV LLTQENPFLS KFSKETQKQA
660 670 680 690 700
NNFDMGSIVL KYEPDPQEPE TLQCPIVLCG WRGKTSIRSF VPKNERLHYL
710 720 730 740 750
RMMGVEVFKE KAEVLEKKPV EGKACDEEHI DEKMDIDGAK EESKELSGNE
760 770 780 790
SGDDEDPKEE DVIDRGVLEH VALKNTSAIP ASVEDQAEDA SVSKESVD
Length:798
Mass (Da):91,020
Last modified:April 4, 2006 - v1
Checksum:i3F79B94FBDED8AA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR848434 mRNA. Translation: CAJ83692.1.
RefSeqiNP_001015962.1. NM_001015962.2.
XP_012820119.1. XM_012964665.1.
XP_012820120.1. XM_012964666.1.
UniGeneiStr.20500.

Genome annotation databases

GeneIDi548716.
KEGGixtr:548716.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CR848434 mRNA. Translation: CAJ83692.1.
RefSeqiNP_001015962.1. NM_001015962.2.
XP_012820119.1. XM_012964665.1.
XP_012820120.1. XM_012964666.1.
UniGeneiStr.20500.

3D structure databases

ProteinModelPortaliQ28E61.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi8364.ENSXETP00000059424.

Proteomic databases

PaxDbiQ28E61.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi548716.
KEGGixtr:548716.

Organism-specific databases

CTDi54888.
XenbaseiXB-GENE-962623. nsun2.

Phylogenomic databases

eggNOGiKOG2198. Eukaryota.
COG0144. LUCA.
HOGENOMiHOG000205147.
HOVERGENiHBG106711.
InParanoidiQ28E61.
KOiK15335.

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR001678. MeTrfase_RsmB/NOP2.
IPR023267. RCMT.
IPR023270. RCMT_NCL1.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PfamiPF01189. Methyltr_RsmB-F. 1 hit.
[Graphical view]
PRINTSiPR02008. RCMTFAMILY.
PR02011. RCMTNCL1.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS51686. SAM_MT_RSMB_NOP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Sanger Xenopus tropicalis EST/cDNA project
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Egg.

Entry informationi

Entry nameiNSUN2_XENTR
AccessioniPrimary (citable) accession number: Q28E61
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: April 4, 2006
Last modified: May 11, 2016
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.