Q28DZ1 (IKKA_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inhibitor of nuclear factor kappa-B kinase subunit alpha
Short name=I kappa-B kinase alpha
Short name=IKK-A
Short name=IKK-alpha
Short name=IkBKA
Short name=IkappaB kinase
EC=2.7.11.10

Alternative name(s):
Conserved helix-loop-helix ubiquitous kinase
Nuclear factor NF-kappa-B inhibitor kinase alpha
Short name=NFKBIKA

Gene names

Name:	chuk
Synonyms:	ikka
ORF Names:	TEgg047h13.1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Amphibia › Batrachia › Anura › Mesobatrachia › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	743 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines By similarity.
Catalytic activity	ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein].
Enzyme regulation	Activated when phosphorylated and inactivated when dephosphorylated By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity. Note: Shuttles between the cytoplasm and the nucleus By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW IkappaB kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 743	743	Inhibitor of nuclear factor kappa-B kinase subunit alpha		PRO_0000268829
Regions
	Domain	15 – 300	286	Protein kinase
	Domain	453 – 474	22	Leucine-zipper
	Nucleotide binding	21 – 29	9	ATP By similarity
	Region	736 – 741	6	NEMO-binding By similarity
Sites
	Active site	144	1	Proton acceptor By similarity
	Binding site	44	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q28DZ1 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 2ED9B09747636CDE	FASTA	743	85,088
	10 20 30 40 50 60 MEKPPVQHGA VCGPWDMKDR LGTGGFGNVC LYQNRETGEL KAIKSCRLEL SMKNKERWCQ 70 80 90 100 110 120 EIQIMKRLNH PNVVKACEVP PEMDFLVNDV PHLAMEYCAG GDLRKLLNKP ENCCGLKESQ 130 140 150 160 170 180 VLNLMSDIGS GIQYLHEKRI IHRDLKPENI VLQECNGKTV HKIIDLGYAK DLDQGSLCTS 190 200 210 220 230 240 FVGTLQYLAP ELFENKPYSV TVDYWSFGTM VFECIAGFRP FLHNMQPFTW HEKIKKKDHK 250 260 270 280 290 300 HIYAYEEMSG EIRFSSYLPQ PNNLCSIIVD KIETWLQLLL NWDPIQRGGG MDCGRPQCFV 310 320 330 340 350 360 LMDQILNLKI VHILNMTSAK IHSFHLQPEE SLHTLQSRIE TETGISTCNQ ELLLEMGVSL 370 380 390 400 410 420 DPRKPASQCV IDGVKGWDSY MVYLFDKSKT VYEGPFQSRS LSECLNYIVQ DSKVQLPIPQ 430 440 450 460 470 480 LRKVWAEAVH YVSGLKEDYS RLFQGQRAAM LSLLRFNTNL TKMKNTMVSA SQQLGAKLQF 490 500 510 520 530 540 FKRSIEIDLE RYSDQMAYGI SSEKMLRAWK EMEDKAVCFG KAGKIHFLDE TIMGLHTEIV 550 560 570 580 590 600 ELQKSPCARR QGDVMEHLEQ RAMELYKQLK PRSPDKAYSD STEMVKILVQ TVQGQDRVLK 610 620 630 640 650 660 ELFGHLSKLL GCKQKIIDLL PQIEMTVNNI KEADSSLMQM QAKRQREIWH LLKIACTQSS 670 680 690 700 710 720 ARSLGPASVE TPITPRTSAW PDQSSSQALF SMLTTKDKSR EILRHTIDKN SDYQKLLSSL 730 740 ILQTQTTMEQ NSLMSLDFSW LNK

References

[1]	Sanger Xenopus tropicalis EST/cDNA project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Egg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR848527 mRNA. Translation: CAJ83676.1.
RefSeq	NP_001016900.1. NM_001016900.2.
UniGene	Str.19041.
3D structure databases
ProteinModelPortal	Q28DZ1.
ModBase	Search...
Protein-protein interaction databases
STRING	Q28DZ1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	549654.
KEGG	xtr:549654.
Organism-specific databases
CTD	1147.
Xenbase	XB-GENE-5910008. chuk.
Phylogenomic databases
GeneTree	ENSGT00530000063051.
HOVERGEN	HBG018241.
Gene expression databases
Bgee	Q28DZ1.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017442. Se/Thr_kinase-like_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
KO	K04467.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IKKA_XENTR

Accession

Primary (citable) accession number: Q28DZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 12, 2006
Last sequence update:	April 4, 2006
Last modified:	November 16, 2011
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program