ID   CISY_XENTR              Reviewed;         468 AA.
AC   Q28DK1; A0JPE2;
DT   17-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT   04-APR-2006, sequence version 1.
DT   27-MAR-2024, entry version 100.
DE   RecName: Full=Citrate synthase, mitochondrial;
DE            EC=2.3.3.1;
DE   AltName: Full=Citrate (Si)-synthase;
DE   Flags: Precursor;
GN   Name=cs; ORFNames=TNeu053d23.1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Neurula;
RG   Sanger Xenopus tropicalis EST/cDNA project;
RL   Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=N6; TISSUE=Skeletal muscle;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (NOV-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + H2O + oxaloacetate = citrate + CoA + H(+);
CC         Xref=Rhea:RHEA:16845, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16452, ChEBI:CHEBI:16947, ChEBI:CHEBI:57287,
CC         ChEBI:CHEBI:57288; EC=2.3.3.1; Evidence={ECO:0000255|PROSITE-
CC         ProRule:PRU10117};
CC   -!- PATHWAY: Carbohydrate metabolism; tricarboxylic acid cycle; isocitrate
CC       from oxaloacetate: step 1/2.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250}.
CC   -!- MISCELLANEOUS: Citrate synthase is found in nearly all cells capable of
CC       oxidative metabolism.
CC   -!- SIMILARITY: Belongs to the citrate synthase family. {ECO:0000305}.
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DR   EMBL; CR855469; CAJ83375.1; -; mRNA.
DR   EMBL; BC127380; AAI27381.1; -; mRNA.
DR   RefSeq; NP_001017313.1; NM_001017313.3.
DR   AlphaFoldDB; Q28DK1; -.
DR   SMR; Q28DK1; -.
DR   STRING; 8364.ENSXETP00000014746; -.
DR   PaxDb; 8364-ENSXETP00000020990; -.
DR   DNASU; 550067; -.
DR   Ensembl; ENSXETT00000020990; ENSXETP00000020990; ENSXETG00000009506.
DR   GeneID; 550067; -.
DR   KEGG; xtr:550067; -.
DR   AGR; Xenbase:XB-GENE-943160; -.
DR   CTD; 1431; -.
DR   Xenbase; XB-GENE-943160; cs.
DR   eggNOG; KOG2617; Eukaryota.
DR   HOGENOM; CLU_022049_2_1_1; -.
DR   InParanoid; Q28DK1; -.
DR   OMA; VLEWLFK; -.
DR   OrthoDB; 3513214at2759; -.
DR   PhylomeDB; Q28DK1; -.
DR   TreeFam; TF300398; -.
DR   Reactome; R-XTR-71403; Citric acid cycle (TCA cycle).
DR   UniPathway; UPA00223; UER00717.
DR   Proteomes; UP000008143; Chromosome 2.
DR   Bgee; ENSXETG00000009506; Expressed in skeletal muscle tissue and 17 other cell types or tissues.
DR   ExpressionAtlas; Q28DK1; baseline.
DR   GO; GO:0005759; C:mitochondrial matrix; ISS:UniProtKB.
DR   GO; GO:0004108; F:citrate (Si)-synthase activity; ISS:UniProtKB.
DR   GO; GO:0005975; P:carbohydrate metabolic process; ISS:UniProtKB.
DR   GO; GO:0006101; P:citrate metabolic process; IEA:InterPro.
DR   GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-UniPathway.
DR   CDD; cd06105; ScCit1-2_like; 1.
DR   Gene3D; 1.10.580.10; Citrate Synthase, domain 1; 1.
DR   Gene3D; 1.10.230.10; Cytochrome P450-Terp, domain 2; 1.
DR   InterPro; IPR016142; Citrate_synth-like_lrg_a-sub.
DR   InterPro; IPR016143; Citrate_synth-like_sm_a-sub.
DR   InterPro; IPR002020; Citrate_synthase.
DR   InterPro; IPR019810; Citrate_synthase_AS.
DR   InterPro; IPR010109; Citrate_synthase_euk.
DR   InterPro; IPR036969; Citrate_synthase_sf.
DR   NCBIfam; TIGR01793; cit_synth_euk; 1.
DR   PANTHER; PTHR11739; CITRATE SYNTHASE; 1.
DR   PANTHER; PTHR11739:SF8; CITRATE SYNTHASE, MITOCHONDRIAL; 1.
DR   Pfam; PF00285; Citrate_synt; 1.
DR   PRINTS; PR00143; CITRTSNTHASE.
DR   SUPFAM; SSF48256; Citrate synthase; 1.
DR   PROSITE; PS00480; CITRATE_SYNTHASE; 1.
PE   2: Evidence at transcript level;
KW   Mitochondrion; Reference proteome; Transferase; Transit peptide;
KW   Tricarboxylic acid cycle.
FT   TRANSIT         1..30
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000250"
FT   CHAIN           31..468
FT                   /note="Citrate synthase, mitochondrial"
FT                   /id="PRO_0000253910"
FT   ACT_SITE        303
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        349
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
FT   ACT_SITE        404
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10117"
SQ   SEQUENCE   468 AA;  51834 MW;  228AAC80C8D20813 CRC64;
     MSLITAGRLC ARILGAKNSP CALIAARQAS SSANLKDVLS DLIPKEQTRI KNFRQQYGKN
     VIGQITVDMM YGGMRGMKGL VYETSVLDPD EGIRFRGYSI PECQKLLPKA PGGEEPLPEG
     LFWLLVTGEA PSQEQVNWIS KEWAKRAALP SHVVTMLDNF PTNLHPMSQL SAAITALNSE
     SNFARGYAEG VNKTKYWELI YEDSMDLIAK LPCVAAKIYR NLYREGSSIG AIDSNLDWSH
     NFTNMLGYTD PQFTELMRLY LTIHSDHEGG NVSAHTSHLV GSALSDPYLS FSAAMNGLAG
     PLHGLANQEV LVWLTSLQKD LGGEVSDEKL RDYIWNTLNS GRVVPGYGHA VLRKTDPRYT
     CQREFALKHL PNDPMFKLVA QLYKIVPNVL LEQGKAKNPW PNVDAHSGVL LQYYGMKEMN
     YYTVLFGVSR ALGVLAQLIW SRALGFPLER PKSMSTDGLM QLVGSKSG
//