Q28D84 (EZH2_XENTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Histone-lysine N-methyltransferase EZH2
EC=2.1.1.43

Alternative name(s):
Enhancer of zeste homolog 2

Gene names

Name:	ezh2
ORF Names:	TGas092f05.1

Organism

Xenopus tropicalis (Western clawed frog) (Silurana tropicalis) [Reference proteome]

Taxonomic identifier

8364 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Pipoidea › Pipidae › Xenopodinae › Xenopus › Silurana

Protein attributes

Sequence length	748 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Polycomb group (PcG) protein By similarity. Catalytic subunit of the prc2/eed-ezh2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene By similarity.
Catalytic activity	S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
Subunit structure	Component of the prc2/eed-ezh2 complex By similarity.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the histone-lysine methyltransferase family. EZ subfamily. Contains 1 CXC domain. Contains 1 SET domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Chromatin regulator Methyltransferase Repressor Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	histone lysine methylation Inferred from electronic annotation. Source: GOC regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	ESC/E(Z) complex Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	chromatin binding Inferred from electronic annotation. Source: InterPro histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 748	748	Histone-lysine N-methyltransferase EZH2		PRO_0000345430
Regions
	Domain	505 – 607	103	CXC
	Domain	613 – 733	121	SET
	Compositional bias	183 – 211	29	Asp-rich
	Compositional bias	525 – 590	66	Cys-rich

Sequences

	Sequence		Length	Mass (Da)
	Q28D84 [UniParc]. Last modified April 4, 2006. Version 1. Checksum: 0D6D3FDE6C329CD1	FASTA	748	85,630
	10 20 30 40 50 60 MGQTGKKSEK GPVCWRKRVK SEYMRLRQLK RFRRADEVKS MFNTNRQKIM ERTEILNQEW 70 80 90 100 110 120 KQRRIQPVHI MTTVSSLRGT RECSVTSDLD FPKQVIPLKT LTAVASVPIM YSWSPLQQNF 130 140 150 160 170 180 MVEDETVLHN IPYMGDEVLD QDGTFIEELI KNYDGKVHGD RECGFINDEI FVELVNALAQ 190 200 210 220 230 240 YSDYEDDEDG DDNQDDERDD TAKDQDDNME DQETQPLRKF PSDKIFEAIS SMFPDKGTLE 250 260 270 280 290 300 ELKEKYKELT EQQLPGALPP ECTPNIDGPN AKSVQREQSL HSFHTLFCRR CFKYDCFLHP 310 320 330 340 350 360 FHATPNTYKR KNNEAANDGK PCGPHCYQLL EGAREFAAAL TAERIKTPPK RPSGRRRGRL 370 380 390 400 410 420 PNNTSRPSTP TVNVLEAKDT DSDREAGTET GGESNDKEEE EKKDETSSSS EANSRCQTPI 430 440 450 460 470 480 KMKPNIEPPE NVEWSGAEAS LFRVLIGTYY DNFCAIARLI GTKTCRQVYE FRVKESSIIA 490 500 510 520 530 540 PVIAEDVDTP PRKKKRKHRL WAAHCRKIQL KKDGSSNHVY NYQPCDHPRQ PCDSSCPCVI 550 560 570 580 590 600 AQNFCEKFCQ CSSECQNRFP GCRCKAQCNT KQCPCYLAVR ECDPDLCLTC GAADHWDSKN 610 620 630 640 650 660 VSCKNCSIQR GSKKHLLLAP SDVAGWGIFI KDPVQKNEFI SEYCGEIISQ DEADRRGKVY 670 680 690 700 710 720 DKYMCSFLFN LNNDFVVDAT RKGNKIRFAN HSVNPNCYAK VMMVNGDHRI GIFAKRAIQT 730 740 GEELFFDYRY SQADALKYVG IEREMEIP

References

[1]	Sanger Xenopus tropicalis EST/cDNA project Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Gastrula.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CR855647 mRNA. Translation: CAJ83863.1.
RefSeq	NP_001017293.1. NM_001017293.2.
UniGene	Str.1297.
3D structure databases
ProteinModelPortal	Q28D84.
SMR	Q28D84. Positions 40-68.
ModBase	Search...
Protein-protein interaction databases
STRING	8364.ENSXETP00000043460.
Proteomic databases
PaxDb	Q28D84.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	550047.
KEGG	xtr:550047.
Organism-specific databases
CTD	2146.
Xenbase	XB-GENE-956215. ezh2.
Phylogenomic databases
eggNOG	NOG303400.
HOVERGEN	HBG002453.
KO	K11430.
Family and domain databases
InterPro	IPR026489. CXC_dom. IPR021654. EZH2_WD-Binding. IPR001005. SANT/Myb. IPR001214. SET_dom. [Graphical view]
Pfam	PF11616. EZH2_WD-Binding. 1 hit. PF00856. SET. 1 hit. [Graphical view]
SMART	SM00717. SANT. 2 hits. SM00317. SET. 1 hit. [Graphical view]
PROSITE	PS51633. CXC. 1 hit. PS50280. SET. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

EZH2_XENTR

Accession

Primary (citable) accession number: Q28D84

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	April 4, 2006
Last modified:	April 3, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program