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Q28CN3

- UBP33_XENTR

UniProt

Q28CN3 - UBP33_XENTR

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Protein

Ubiquitin carboxyl-terminal hydrolase 33

Gene
usp33, TEgg041n23.1
Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of ccp110 in S and G2/M phase, leading to stabilize ccp110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of robo1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of robo1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (arrb1 and arrb2) and beta-2 adrenergic receptor (adrb2). Deubiquitinates dio2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei157 – 1571Nucleophile By similarity
Active sitei623 – 6231Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri28 – 9265UBP-typeAdd
BLAST

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: UniProtKB
  2. ubiquitin-specific protease activity Source: UniProtKB
  3. ubiquitin thiolesterase activity Source: UniProtKB
  4. zinc ion binding Source: UniProtKB

GO - Biological processi

  1. axon guidance Source: UniProtKB
  2. cell migration Source: UniProtKB
  3. centrosome duplication Source: UniProtKB
  4. endocytosis Source: UniProtKB-KW
  5. protein deubiquitination Source: UniProtKB
  6. protein K48-linked deubiquitination Source: UniProtKB
  7. protein K63-linked deubiquitination Source: UniProtKB
  8. regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
  9. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Endocytosis, Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Protein family/group databases

MEROPSiC19.037.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 33 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 33
Ubiquitin thioesterase 33
Ubiquitin-specific-processing protease 33
Gene namesi
Name:usp33
ORF Names:TEgg041n23.1
OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Taxonomic identifieri8364 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
ProteomesiUP000008143: Unplaced

Organism-specific databases

XenbaseiXB-GENE-1016674. usp33.

Subcellular locationi

Cytoplasmperinuclear region By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
Note: Associates with centrosomes predominantly in S and G2 phases but less in G1 phase By similarity.

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. perinuclear region of cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 892892Ubiquitin carboxyl-terminal hydrolase 33PRO_0000390427Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi8364.ENSXETP00000028749.

Structurei

3D structure databases

ProteinModelPortaliQ28CN3.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini148 – 665518USPAdd
BLAST
Domaini667 – 76094DUSP 1Add
BLAST
Domaini768 – 871104DUSP 2Add
BLAST

Domaini

The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue By similarity.

Sequence similaritiesi

Contains 2 DUSP domains.
Contains 1 USP domain.

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5560.
HOGENOMiHOG000286031.
HOVERGENiHBG054196.
KOiK11848.
OrthoDBiEOG7CRTP2.
TreeFamiTF352179.

Family and domain databases

Gene3Di3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProiIPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view]
PfamiPF06337. DUSP. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view]
SMARTiSM00695. DUSP. 2 hits.
[Graphical view]
SUPFAMiSSF143791. SSF143791. 2 hits.
PROSITEiPS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q28CN3-1 [UniParc]FASTAAdd to Basket

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MSSLVCDCPH LESVGEVTKE ELIKKSHGSC QDCRVRGPNL WACLENGCSY    50
VGCGESHVDH STLHSQDTKH CLTVNLTTLR VWCYTCSKEV FLDRKLSTQP 100
TSAKLQDSHT QESKMSSSLT LKIPAAVVSE NLDIELEEED ELKTRGLTGL 150
KNIGNTCYMN AALQALSNCP PLTHYFLDCG GLARTDKKPA LCKSYQKLMS 200
DIWHKNRPGF VIPTNLFQGI KSVNPTFRGY SQQDAQEFLR CLMDVLHEEL 250
KEQIVEVEED AQTGIVEENL DEDKSQSDND FHSCDSGSSS DHAESESRKL 300
SEELTESTML IHEDQKDVES CKTWQKEKKF SNNLNQNHFL QDFEKNIQST 350
IEESECLKQE TVKVQIQSKA ADFTAEVNMN DLPTSQTPLL NEGATTHLSS 400
SPPKPGAVWT GHKKVPGLCP AKKRKQKKYH SVIADIFDGT IVSSVQCLTC 450
DRVSVTLETF QDLSLPIPGK EDLAKLHSSS HQSSLVKAGS CGEAYAPQGW 500
IAFFLEYFKS WFWGPTVTLQ DCLAAFFARD ELKGDNMYSC EKCKKLRNGV 550
KFCKVQKFPE ILCIHLKRFR HELMFSTKIG THVSFPLEGL DLQPFLAKDS 600
PSQIVTYDLL SVICHHGTAS SGHYIAYCRN NLNNLWYEFD DQSVTEVSEA 650
TVQNAEAYVL FYRKASEEAQ KERRRVSCLL NIMEPSLLQF YISRQWLNKF 700
KTFAEPGPIS NNDFLCIHGG VPPRKASFIE DLVVMLPQNI WDYLYSRYGG 750
GPAVNHLYVC YTCQTEMEKI EKRRKMELET FIRLNKAFQE EESPAVIYCI 800
SMQWFREWEG FVKSKDSDPP GPIDNTKIAA AKCGHITLRQ GADSGQISEE 850
TWLFLQSIYG GGPEITLRQN VTLAESEGGH AEEKIDVETR NI 892
Length:892
Mass (Da):100,733
Last modified:May 29, 2013 - v2
Checksum:i8605699C7AB5B8AC
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti320 – 3201S → I in CAJ82183. 1 Publication
Sequence conflicti671 – 6711K → I in AAI71081. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR926290 mRNA. Translation: CAJ82183.1.
AAMC01095352 Genomic DNA. No translation available.
AAMC01095353 Genomic DNA. No translation available.
BC171081 mRNA. Translation: AAI71081.1.
RefSeqiNP_001016228.1. NM_001016228.2.
UniGeneiStr.42260.

Genome annotation databases

GeneIDi548982.
KEGGixtr:548982.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CR926290 mRNA. Translation: CAJ82183.1 .
AAMC01095352 Genomic DNA. No translation available.
AAMC01095353 Genomic DNA. No translation available.
BC171081 mRNA. Translation: AAI71081.1 .
RefSeqi NP_001016228.1. NM_001016228.2.
UniGenei Str.42260.

3D structure databases

ProteinModelPortali Q28CN3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 8364.ENSXETP00000028749.

Protein family/group databases

MEROPSi C19.037.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 548982.
KEGGi xtr:548982.

Organism-specific databases

CTDi 23032.
Xenbasei XB-GENE-1016674. usp33.

Phylogenomic databases

eggNOGi COG5560.
HOGENOMi HOG000286031.
HOVERGENi HBG054196.
KOi K11848.
OrthoDBi EOG7CRTP2.
TreeFami TF352179.

Family and domain databases

Gene3Di 3.30.2230.10. 2 hits.
3.30.40.10. 1 hit.
InterProi IPR006615. Pept_C19_DUSP.
IPR018200. Pept_C19ubi-hydrolase_C_CS.
IPR001394. Peptidase_C19_UCH.
IPR028889. UCH/PAN2.
IPR013083. Znf_RING/FYVE/PHD.
IPR001607. Znf_UBP.
[Graphical view ]
Pfami PF06337. DUSP. 2 hits.
PF00443. UCH. 1 hit.
PF02148. zf-UBP. 1 hit.
[Graphical view ]
SMARTi SM00695. DUSP. 2 hits.
[Graphical view ]
SUPFAMi SSF143791. SSF143791. 2 hits.
PROSITEi PS51283. DUSP. 2 hits.
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
PS50271. ZF_UBP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Sanger Xenopus tropicalis EST/cDNA project
    Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Egg.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. NIH - Xenopus Gene Collection (XGC) project
    Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Neurula.

Entry informationi

Entry nameiUBP33_XENTR
AccessioniPrimary (citable) accession number: Q28CN3
Secondary accession number(s): B7ZU27, F7EIJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2009
Last sequence update: May 29, 2013
Last modified: April 16, 2014
This is version 53 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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