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Q28CN3

- UBP33_XENTR

UniProt

Q28CN3 - UBP33_XENTR

Protein

Ubiquitin carboxyl-terminal hydrolase 33

Gene

usp33

Organism
Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 54 (01 Oct 2014)
      Sequence version 2 (29 May 2013)
      Previous versions | rss
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    Functioni

    Deubiquitinating enzyme involved in various processes such as centrosome duplication, cellular migration and beta-2 adrenergic receptor/ADRB2 recycling. Involved in regulation of centrosome duplication by mediating deubiquitination of ccp110 in S and G2/M phase, leading to stabilize ccp110 during the period which centrioles duplicate and elongate. Involved in cell migration via its interaction with intracellular domain of robo1, leading to regulate the Slit signaling. Plays a role in commissural axon guidance cross the ventral midline of the neural tube in a Slit-dependent manner, possibly by mediating the deubiquitination of robo1. Acts as a regulator of G-protein coupled receptor (GPCR) signaling by mediating the deubiquitination of beta-arrestins (arrb1 and arrb2) and beta-2 adrenergic receptor (adrb2). Deubiquitinates dio2, thereby regulating thyroid hormone regulation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains By similarity.By similarity

    Catalytic activityi

    Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei157 – 1571NucleophilePROSITE-ProRule annotation
    Active sitei623 – 6231Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. cysteine-type endopeptidase activity Source: UniProtKB
    2. ubiquitin-specific protease activity Source: UniProtKB
    3. ubiquitin thiolesterase activity Source: UniProtKB
    4. zinc ion binding Source: UniProtKB

    GO - Biological processi

    1. axon guidance Source: UniProtKB
    2. cell migration Source: UniProtKB
    3. centrosome duplication Source: UniProtKB
    4. endocytosis Source: UniProtKB-KW
    5. protein deubiquitination Source: UniProtKB
    6. protein K48-linked deubiquitination Source: UniProtKB
    7. protein K63-linked deubiquitination Source: UniProtKB
    8. regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
    9. ubiquitin-dependent protein catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Keywords - Biological processi

    Endocytosis, Ubl conjugation pathway

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiC19.037.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin carboxyl-terminal hydrolase 33 (EC:3.4.19.12)
    Alternative name(s):
    Deubiquitinating enzyme 33
    Ubiquitin thioesterase 33
    Ubiquitin-specific-processing protease 33
    Gene namesi
    Name:usp33
    ORF Names:TEgg041n23.1
    OrganismiXenopus tropicalis (Western clawed frog) (Silurana tropicalis)
    Taxonomic identifieri8364 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusSilurana
    ProteomesiUP000008143: Unplaced

    Organism-specific databases

    XenbaseiXB-GENE-1016674. usp33.

    Subcellular locationi

    Cytoplasmperinuclear region By similarity. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
    Note: Associates with centrosomes predominantly in S and G2 phases but less in G1 phase.By similarity

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. perinuclear region of cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 892892Ubiquitin carboxyl-terminal hydrolase 33PRO_0000390427Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi8364.ENSXETP00000028749.

    Structurei

    3D structure databases

    ProteinModelPortaliQ28CN3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini148 – 665518USPAdd
    BLAST
    Domaini667 – 76094DUSP 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini768 – 871104DUSP 2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The UBP-type zinc finger binds 3 zinc ions. However, it does not bind ubiquitin, probably because the conserved Arg in position 55 is replaced by a Glu residue By similarity.By similarity

    Sequence similaritiesi

    Contains 2 DUSP domains.PROSITE-ProRule annotation
    Contains 1 UBP-type zinc finger.PROSITE-ProRule annotation
    Contains 1 USP domain.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri28 – 9265UBP-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5560.
    HOGENOMiHOG000286031.
    HOVERGENiHBG054196.
    KOiK11848.
    OrthoDBiEOG7CRTP2.
    TreeFamiTF352179.

    Family and domain databases

    Gene3Di3.30.2230.10. 2 hits.
    3.30.40.10. 1 hit.
    InterProiIPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view]
    PfamiPF06337. DUSP. 2 hits.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view]
    SMARTiSM00695. DUSP. 2 hits.
    [Graphical view]
    SUPFAMiSSF143791. SSF143791. 2 hits.
    PROSITEiPS51283. DUSP. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q28CN3-1 [UniParc]FASTAAdd to Basket

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    MSSLVCDCPH LESVGEVTKE ELIKKSHGSC QDCRVRGPNL WACLENGCSY    50
    VGCGESHVDH STLHSQDTKH CLTVNLTTLR VWCYTCSKEV FLDRKLSTQP 100
    TSAKLQDSHT QESKMSSSLT LKIPAAVVSE NLDIELEEED ELKTRGLTGL 150
    KNIGNTCYMN AALQALSNCP PLTHYFLDCG GLARTDKKPA LCKSYQKLMS 200
    DIWHKNRPGF VIPTNLFQGI KSVNPTFRGY SQQDAQEFLR CLMDVLHEEL 250
    KEQIVEVEED AQTGIVEENL DEDKSQSDND FHSCDSGSSS DHAESESRKL 300
    SEELTESTML IHEDQKDVES CKTWQKEKKF SNNLNQNHFL QDFEKNIQST 350
    IEESECLKQE TVKVQIQSKA ADFTAEVNMN DLPTSQTPLL NEGATTHLSS 400
    SPPKPGAVWT GHKKVPGLCP AKKRKQKKYH SVIADIFDGT IVSSVQCLTC 450
    DRVSVTLETF QDLSLPIPGK EDLAKLHSSS HQSSLVKAGS CGEAYAPQGW 500
    IAFFLEYFKS WFWGPTVTLQ DCLAAFFARD ELKGDNMYSC EKCKKLRNGV 550
    KFCKVQKFPE ILCIHLKRFR HELMFSTKIG THVSFPLEGL DLQPFLAKDS 600
    PSQIVTYDLL SVICHHGTAS SGHYIAYCRN NLNNLWYEFD DQSVTEVSEA 650
    TVQNAEAYVL FYRKASEEAQ KERRRVSCLL NIMEPSLLQF YISRQWLNKF 700
    KTFAEPGPIS NNDFLCIHGG VPPRKASFIE DLVVMLPQNI WDYLYSRYGG 750
    GPAVNHLYVC YTCQTEMEKI EKRRKMELET FIRLNKAFQE EESPAVIYCI 800
    SMQWFREWEG FVKSKDSDPP GPIDNTKIAA AKCGHITLRQ GADSGQISEE 850
    TWLFLQSIYG GGPEITLRQN VTLAESEGGH AEEKIDVETR NI 892
    Length:892
    Mass (Da):100,733
    Last modified:May 29, 2013 - v2
    Checksum:i8605699C7AB5B8AC
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti320 – 3201S → I in CAJ82183. 1 PublicationCurated
    Sequence conflicti671 – 6711K → I in AAI71081. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR926290 mRNA. Translation: CAJ82183.1.
    AAMC01095352 Genomic DNA. No translation available.
    AAMC01095353 Genomic DNA. No translation available.
    BC171081 mRNA. Translation: AAI71081.1.
    RefSeqiNP_001016228.1. NM_001016228.2.
    UniGeneiStr.42260.

    Genome annotation databases

    GeneIDi548982.
    KEGGixtr:548982.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CR926290 mRNA. Translation: CAJ82183.1 .
    AAMC01095352 Genomic DNA. No translation available.
    AAMC01095353 Genomic DNA. No translation available.
    BC171081 mRNA. Translation: AAI71081.1 .
    RefSeqi NP_001016228.1. NM_001016228.2.
    UniGenei Str.42260.

    3D structure databases

    ProteinModelPortali Q28CN3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 8364.ENSXETP00000028749.

    Protein family/group databases

    MEROPSi C19.037.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 548982.
    KEGGi xtr:548982.

    Organism-specific databases

    CTDi 23032.
    Xenbasei XB-GENE-1016674. usp33.

    Phylogenomic databases

    eggNOGi COG5560.
    HOGENOMi HOG000286031.
    HOVERGENi HBG054196.
    KOi K11848.
    OrthoDBi EOG7CRTP2.
    TreeFami TF352179.

    Family and domain databases

    Gene3Di 3.30.2230.10. 2 hits.
    3.30.40.10. 1 hit.
    InterProi IPR006615. Pept_C19_DUSP.
    IPR018200. Pept_C19ubi-hydrolase_C_CS.
    IPR001394. Peptidase_C19_UCH.
    IPR028889. UCH/PAN2.
    IPR013083. Znf_RING/FYVE/PHD.
    IPR001607. Znf_UBP.
    [Graphical view ]
    Pfami PF06337. DUSP. 2 hits.
    PF00443. UCH. 1 hit.
    PF02148. zf-UBP. 1 hit.
    [Graphical view ]
    SMARTi SM00695. DUSP. 2 hits.
    [Graphical view ]
    SUPFAMi SSF143791. SSF143791. 2 hits.
    PROSITEi PS51283. DUSP. 2 hits.
    PS00972. USP_1. 1 hit.
    PS00973. USP_2. 1 hit.
    PS50235. USP_3. 1 hit.
    PS50271. ZF_UBP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Sanger Xenopus tropicalis EST/cDNA project
      Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Egg.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. NIH - Xenopus Gene Collection (XGC) project
      Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Neurula.

    Entry informationi

    Entry nameiUBP33_XENTR
    AccessioniPrimary (citable) accession number: Q28CN3
    Secondary accession number(s): B7ZU27, F7EIJ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 2009
    Last sequence update: May 29, 2013
    Last modified: October 1, 2014
    This is version 54 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3