ID   MESH1_XENTR             Reviewed;         179 AA.
AC   Q28C98;
DT   12-DEC-2006, integrated into UniProtKB/Swiss-Prot.
DT   04-APR-2006, sequence version 1.
DT   27-MAR-2024, entry version 81.
DE   RecName: Full=Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase MESH1;
DE            EC=3.1.7.2;
DE   AltName: Full=HD domain-containing protein 3;
DE   AltName: Full=Metazoan SpoT homolog 1;
DE            Short=MESH1;
DE   AltName: Full=Penta-phosphate guanosine-3'-pyrophosphohydrolase;
DE            Short=(ppGpp)ase;
GN   Name=hddc3; Synonyms=Mesh1; ORFNames=TGas061b07.1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Gastrula;
RG   Sanger Xenopus tropicalis EST/cDNA project;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: ppGpp hydrolyzing enzyme involved in starvation response.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=guanosine 3',5'-bis(diphosphate) + H2O = diphosphate + GDP +
CC         H(+); Xref=Rhea:RHEA:14253, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58189, ChEBI:CHEBI:77828; EC=3.1.7.2;
CC   -!- COFACTOR:
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000250};
CC   -!- SIMILARITY: Belongs to the MESH1 family. {ECO:0000305}.
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DR   EMBL; CR942384; CAJ81555.1; -; mRNA.
DR   RefSeq; NP_001039099.1; NM_001045634.1.
DR   AlphaFoldDB; Q28C98; -.
DR   SMR; Q28C98; -.
DR   STRING; 8364.ENSXETP00000025551; -.
DR   PaxDb; 8364-ENSXETP00000021510; -.
DR   Ensembl; ENSXETT00000112921; ENSXETP00000106490; ENSXETG00000009763.
DR   GeneID; 733918; -.
DR   KEGG; xtr:733918; -.
DR   AGR; Xenbase:XB-GENE-973216; -.
DR   CTD; 374659; -.
DR   Xenbase; XB-GENE-973216; hddc3.
DR   eggNOG; KOG1157; Eukaryota.
DR   HOGENOM; CLU_084517_1_0_1; -.
DR   InParanoid; Q28C98; -.
DR   OMA; PPWRERK; -.
DR   OrthoDB; 2783605at2759; -.
DR   Proteomes; UP000008143; Chromosome 8.
DR   Bgee; ENSXETG00000009763; Expressed in testis and 13 other cell types or tissues.
DR   GO; GO:0008893; F:guanosine-3',5'-bis(diphosphate) 3'-diphosphatase activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd00077; HDc; 1.
DR   Gene3D; 1.10.3210.10; Hypothetical protein af1432; 1.
DR   InterPro; IPR003607; HD/PDEase_dom.
DR   InterPro; IPR006674; HD_domain.
DR   PANTHER; PTHR46246; GUANOSINE-3',5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE MESH1; 1.
DR   PANTHER; PTHR46246:SF1; GUANOSINE-3',5'-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE MESH1; 1.
DR   Pfam; PF13328; HD_4; 1.
DR   SMART; SM00471; HDc; 1.
DR   SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR   PROSITE; PS51831; HD; 1.
PE   2: Evidence at transcript level;
KW   Hydrolase; Manganese; Metal-binding; Reference proteome.
FT   CHAIN           1..179
FT                   /note="Guanosine-3',5'-bis(diphosphate) 3'-
FT                   pyrophosphohydrolase MESH1"
FT                   /id="PRO_0000263113"
FT   DOMAIN          32..127
FT                   /note="HD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01175"
FT   ACT_SITE        65
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        66
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250"
FT   BINDING         35
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         61
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         62
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
FT   BINDING         122
FT                   /ligand="Mn(2+)"
FT                   /ligand_id="ChEBI:CHEBI:29035"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   179 AA;  20390 MW;  C0667B09D727D7E2 CRC64;
     MSSDAALILE AANFAAEKHK QQRRKDPEKT PYINHPLGVA RILSHEGGIT DTAVLQAALL
     HDTVEDTNTT FEEIELHFGQ DVRNIVEEVT DDKTLPKMER KRLQMEHAPH CSQKAKLVKL
     ADKLYNLRDL KRCTPEGWSE QRVQEYFQWA SEVVKGLRGT NSILEGKLDH MFMDQGIAV
//